Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme

Zhang, Baoliang; Li, Peitao; Zhang, Hepeng; Wang, Hai; Li, Xiangjie; Tian, Lei; Ali, Nisar; Ali, Zafar; Zhang, Qiuyu

doi:10.1016/j.cej.2016.01.104

Cited by 175 publications

(97 citation statements)

References 42 publications

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“…Besides, using different metal ions as inorganic part, several protein-inorganic hybrid nanoflowers were also synthesized. For example, BSA/ZnO [51], BSA/Zn3(PO4) [52], BSA/Mn3(PO4)2 [53], lipase/ Zn3(PO4)2 [54], lipase/Ca3(PO4)2 [55], phenylphosphinic acid/Al(NO3)3.9H2O [56], BSA/AgNO3 [57], protein/Mn3(PO4)2 [58].…”

Section: Organic-inorganic Hybrid Nanoflowersmentioning

confidence: 98%

A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

Altınkaynak

Tavlasoglu

Özdemir

et al. 2016

Enzyme and Microbial Technology

204

108

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Section: Organic-inorganic Hybrid Nanoflowersmentioning

confidence: 98%

A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

Altınkaynak

Tavlasoglu

Özdemir

et al. 2016

Enzyme and Microbial Technology

204

108

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“…Nanoflowers composed of laccase and copper phosphate were extremely stable and highly active compared to free laccase and were successfully applied to detect phenol in aqueous solution . Some lipases which are widely utilized in biosynthetic industries were also efficiently entrapped within nanoflowers . Furthermore, several groups have utilized hybrid nanoflowers for different applications such as detection of hydrogen peroxide, glucose and protein digestion .…”

Section: Introductionmentioning

confidence: 99%

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

2018

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Herein we report an expeditious and facile sonochemical synthesis method that takes just a few minutes to produce organic‐inorganic hybrid nanoflowers and their application as a nanobiocatalyst. Surfactant treated Burkholderia cepacia lipase was used as the organic component and manganese sulfate as the inorganic component to synthesize the nanoflowers in less than 10 minutes. Careful investigation of the process parameters was performed to obtain the optimized value for each attribute viz. sonication time (7.5 min), lipase concentration (0.1 mg/mL), MnSO4 concentration (150 mM), sonication frequency (33 kHz) and pH (8.0). An additional post‐synthesis treatment of nanoflowers with N‐hydroxysuccinimide (NHS) increased their reusability to over nine cycles with an overall six‐fold increase in the activity. In addition, thermal stability was demonstrated through a twenty‐fold increase in the half‐life of immobilized lipase at 80 °C than in free form. The hybrid nanoflowers displayed a marked improvement in the kinetic resolution of racemic intermediates of metoprolol, a selective β‐1 blocker drug [C (%)=46.7, eep=96.5, E=156] and cloranolol, a non‐selective β‐blocker drug [C (%)=49.5, eep=96.0, E=157] compared to free lipase which signifies its importance as a nanobiocatalyst.

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“…Fe 3 O 4 nanoparticles are a commonly used Fenton catalytic material . Because the nanoparticles contain both Fe 2+ and Fe 3+ , they have superior catalytic performances unlike other Fenton reagents with only one valence state . However, the currently used Fe 3 O 4 Fenton nano‐catalysts often have a small surface area and poor dispersibility.…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic Fe₃O₄ nanoparticles prepared by ferrocene as high‐efficiency heterogeneous Fenton catalyst for the degradation of methyl orange

Chen

Liu

Zhang

et al. 2019

Applied Organom Chemis

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Hydrophilic Fe3O4 nanoparticles were prepared with ferrocene as an iron source via the thermal decomposition method and their catalytic response towards methyl orange was investigated. The effects of the pH, temperature, H2O2 dosage, catalyst dosage and initial dye concentration on the degradation of methyl orange were researched in detail. Furthermore, the stability of the catalyst was evaluated by measuring the degradation rate in eight successive cycles. The study demonstrates that methyl orange can be completely degraded i.e., a 99% degratation rate was obtained within 3 min. This excellent catalytic activity is attributed to the small size and good dispersibility of the nanoparticles, which stimulate the rapid and massive generation of reactive oxygen species in the heterogeneous Fenton reaction. In addition, the magnetic separation of the catalyst offers great prospects for fast and economical decontamination of dye polluted water.

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Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme

Cited by 175 publications

References 42 publications

A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

Hydrophilic Fe₃O₄ nanoparticles prepared by ferrocene as high‐efficiency heterogeneous Fenton catalyst for the degradation of methyl orange

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Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme

Cited by 175 publications

References 42 publications

A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

Hydrophilic Fe3O4 nanoparticles prepared by ferrocene as high‐efficiency heterogeneous Fenton catalyst for the degradation of methyl orange

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Hydrophilic Fe₃O₄ nanoparticles prepared by ferrocene as high‐efficiency heterogeneous Fenton catalyst for the degradation of methyl orange