Preparation of I131-tyrosines and I131-thyroxine

Abdel‐Wahab, M. F.; Megahed, Y. M.

doi:10.1016/0020-708x(66)90061-5

The International Journal of Applied Radiation and Isotopes

1966

DOI: 10.1016/0020-708x(66)90061-5

|View full text |Cite

Preparation of I131-tyrosines and I131-thyroxine

M. F. Abdel‐Wahab¹,

Y. M. Megahed²

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1966

1972

Publication Types

Select...

Article6

Relationship

Self Cite0

Independent6

Authors

Journals

Cited by 10 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Either 131 I as KI or 99m Tc as HTC0 4 were used for labelling, the activities varied from 0.5 to 5 mc. Iodine or technetium were liberated either by the KI + KI0 3 method [9] or by the H 2 0 2 method [10] and the labelling was performed as described in these papers. The products obtained were separated by destination or by elution from a Sephadex G-25 column with tertiary amylalcohol saturated with ammonia [11].…”

mentioning

confidence: 99%

Incorporation of ¹³¹I and ^99mTc in 3, 5, 3'-Triiodothyronine

Abdel‐Wahab¹,

Megahed²

1966

Radiochimica Acta

View full text Add to dashboard Cite

The triiodothyronine thyroid suppression test requires the use of labelled 3,5,3'-trnodothyronine (T 3 ). The uptake of T 3 either by red blood corpuscles [1] or by resin [2] provides a measure for the thyroid function in hyperthyroidism and in other thyroid disorders [3,4], Recently the use of Sephadex G-25 for the separation of 131 I labelled T 3 has been described [5,6,7,8].In connection with recent work in which technetium was used instead of iodine to label amino acids, we have worked out the following methods for labelling T 3 with "Tc. Cold 3,5,3'-trnodothyronine (1-5 mg) was dissolved in 5 Ν NICCHI (0.1-0.5 ml). Either 131 I as KI or 99m Tc as HTC0 4 were used for labelling, the activities varied from 0.5 to 5 mc. Iodine or technetium were liberated either by the KI + KI0 3 method [9] or by the H 2 0 2 method [10] and the labelling was performed as described in these papers. The products obtained were separated by destination or by elution from a Sephadex G-25 column with tertiary amylalcohol saturated with ammonia [11]. The separation of the Tc-labelled fractions by the latter method is shown in Fig. 1. (Iodine-labelled products showed a similar behaviour.) By means of 700 650 600 550 500 s 450 1 400 E 350 υ 300 250 200 150 100 50 ; / J 1 τ, l·' T 3 J I 1 1 -νΊ I ! ,Ί s 20 40 60 Θ0 100 120 140 160 180 200 -Millilitres of solvent Fig. 1. A representative curve for labelling Τ, with Mm Tc, collecting by Shandon fraction collector from a Sephadex-G-25 column. Oxidation with KI/KIO, and elution with tertamyl-alcohol saturated with ammonia. Counting with a wellscintillation counter, flow rate 2 ml/min.paperchromatography we could prove that the labelled T 3 was free from radioactive contaminations. Both methods proved to be satisfactory as is seen from the results in Table 1. However, the better yield was obtained when the separation of labelled triiodothyronine with Sephadex G-25 was used. Table 1. Distribution of radioactivity over the reaction products 181J 131T 99 mT. ± 99m l J l 0 Labelled HA K I+ KIO a Preparation & T dex distillas hadex distillar tion tion 3,5,3 triiodothyronine or 23-46% Tc-diodothyronine 3 I-tyro-sineorTc-51-56% tyrosine 3,5 diiodotyrosine or 3,5 Tc-0 iodotyrosine L-thyroxine 0-25% 58% 20% 22% 57% 34% 9% 28%11% 61%Our results have been confirmed by U.V. spectrophotometry of the separated amino acids and hormones. The confirmation was based on measuring the absorption of each product, comparing the maximum and minimum extinction and matching these results with standards and starting material [12].

show abstract

mentioning

confidence: 99%

Incorporation of ¹³¹I and ^99mTc in 3, 5, 3'-Triiodothyronine

Abdel‐Wahab¹,

Megahed²

1966

Radiochimica Acta

View full text Add to dashboard Cite

show abstract

131I-labelling of tyrosine by iodine monochloride

Hallaba¹,

Elasrag²,

Zeid

1970

The International Journal of Applied Radiation and Isotopes

View full text Add to dashboard Cite

128I and 132I labeled Rose Bengal and thyroxine

Wheeler

Trabal

Lopez-Alonso

1970

The International Journal of Applied Radiation and Isotopes

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Preparation of I131-tyrosines and I131-thyroxine

Cited by 10 publications

References 5 publications

Incorporation of ¹³¹I and ^99mTc in 3, 5, 3'-Triiodothyronine

Incorporation of ¹³¹I and ^99mTc in 3, 5, 3'-Triiodothyronine

131I-labelling of tyrosine by iodine monochloride

128I and 132I labeled Rose Bengal and thyroxine

Contact Info

Product

Resources

About

Preparation of I131-tyrosines and I131-thyroxine

Cited by 10 publications

References 5 publications

Incorporation of 131I and 99mTc in 3, 5, 3'-Triiodothyronine

Incorporation of 131I and 99mTc in 3, 5, 3'-Triiodothyronine

131I-labelling of tyrosine by iodine monochloride

128I and 132I labeled Rose Bengal and thyroxine

Contact Info

Product

Resources

About

Incorporation of ¹³¹I and ^99mTc in 3, 5, 3'-Triiodothyronine

Incorporation of ¹³¹I and ^99mTc in 3, 5, 3'-Triiodothyronine