Preparation of hollow nickel silicate nanospheres for separation of His-tagged proteins

Abstract

Hollow nickel silicate nanospheres (NiSiO3 NSs) with hierarchical shells were hydrothermally synthesized by using silica spheres as a template. The NiSiO3 NSs have an average diameter of 250 nm with a shell thickness of 50 nm, and the hierarchical shell consists of a large number of sheets. By taking advantage of the high affinity of Ni(2+) toward histidine-tagged (His-tagged) proteins, hollow NiSiO3 NSs can be used to enrich and separate His-tagged proteins directly from a mixture of lysed cells. Results indicated that the hollow NiSiO3 NSs presented negligible nonspecific protein adsorption and a high protein binding ability with a high binding capacity of 13.2 mmol g(-1). Their specificity and affinity toward His-tagged proteins remained after recycling 5 times. The hollow NiSiO3 NSs are especially suitable for rapid purification of His-tagged proteins.

Facile synthesis of sea urchin-like magnetic copper silicate hollow spheres for efficient removal of hemoglobin in human blood

“…Similar to that of the Fe 3 O 4 /CuSilicate, and the magnetic nanoparticles were all entrapped by the growing the magnesium, nickel silicate layer, which were proved by the XRD pattern of the as-prepared products between the SiO 2 @Fe 3 O 4 with MgCl 2 , NiCl 2 in alkaline solution ( Figure S4). [21,[27][28][29][30] In addition, the BET of the as-prepared magnetic hierarchical magnesium/nickel silicate hollow nanospheres are 443 m 2 g -1 , 172 m 2 g -1 respectively. Nitrogen adsorption-desorption isotherm of the magnetic magnesium/ nickel silicate hollow spheres are shown in Figure S4(c, f).…”

“…Due to their outstanding structural features, the hollow metal silicates have attracted great attention and have found wide application in fields such as drug delivery, catalyst support, adsorption and protein separation. [18][19][20][21][22][23] Wang etc have prepared MgSiO 3 hollow spheres which exhibited a significantly high storage capacity of drug (2140 mg DOX g −1 ) and held a sustained release property, showing more potential for cancer therapy. [20] Wu etc have also synthesized the hollow nickel silicate spheres with hierarchical shells using similar strategy, which display a strong affinity on His-tagged proteins.…”

“…[20] Wu etc have also synthesized the hollow nickel silicate spheres with hierarchical shells using similar strategy, which display a strong affinity on His-tagged proteins. [21] More recently, Jin etc al have developed a simple route to fabricate hollow rare earth silicates with mesoporous shells which was demonstrated to be good carriers for Au nanocatalysts. [22] These systems, however, still suffer from drawback such as not easy separation.…”

NiS Hollow Spheres for High‐Performance Supercapacitors and Non‐Enzymatic Glucose Sensors

“…[1][2][3][4][5][6] In particular, hollow nanostructures have been extensively studied because of their improved performance and have been successfully applied in gas sensors, photocatalysis, waste water treatment, and energy storage. [7][8][9][10][11][12] To date, there are generally two routes for the preparation of hollow structures, namely using hard templates such as carbon, [13,14] silica, [15,16] and metal oxides, [17,18] and using soft templates such as micelles or vesicles, [19,20] microemulsion droplets, [21,22] and even gas bubbles. [23,24] The hard template approaches commonly suffer from tedious operational procedures, involving template preparation, shell deposition, and removal of templates by acid/base etching reactions or high temperature calcinations, which sometimes cause waste of materials and energy.…”

Highly selective magnetic affinity purification of histidine-tagged proteins by Ni2+ carrying monodisperse composite microspheres

“…[14][15][16][17][18][19][20][21][22] Furthermore, different nanomaterials based on Ni/NiO core/shell nanoparticles, Fe 3 O 4 /Au-ANTA-Co 2+ nanoparticles, nickel silicate nanospheres, and Fe 3 O 4 @NiSiO 3 nanostructures are reported as MOAC based affinity adsorbents for His-tagged protein purication. 6,7,21,23 Recently, Li et al reported a new separation material (SiO 2 @IDA/MAPS nanoparticles) adopting epitope imprinting enhanced IMAC (EI-IMAC) to supply the affinity interaction, which showed good selectivity towards the Histagged recombinant proteins from crude cell lysis. 24 Moreover, magnetic mesoporous silica microspheres exhibit attractive physical and chemical properties as a sorbent, particularly due to their large surface area and associated advantages in diffusion rates, low toxicity, chemically modiable surface and ease of separation under external magnetic elds.…”