Preparation of highly purified cytotrophoblast from human placenta with subsequent modulation to form syncytiotrophoblast in monolayer cultures

Cotte, Carlos; Easty, G. C.; Neville, A. Munro; Monaghan, Paul

doi:10.1007/bf02619191

Cited by 56 publications

(19 citation statements)

References 16 publications

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“…When this is compared with controls, normal tubal epithelial cell growth was affected in some cells as indicated by shortening and swelling of the microvilli. Studies of highly proliferative cells cultured with MTX have shown an increase in cell nuclear size, multinucleated cells and growth inhibition [ 13,14],…”

Section: Discussionmentioning

confidence: 99%

Effects of Methotrexate on Rabbit Oviducts and on Cell Cultures of Bovine Oviduct Epithelium

Tepper

Nahum²,

Rahamim³

et al. 1992

Gynecol Obstet Invest

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The influence of a local injection of 0.05 ml/kg weight of 8 mg/ml methotrexate (MTX) was evaluated in 6 rabbit oviducts and compared with saline injections to the contralateral oviducts. Cell cultures of bovine oviducts were incubated in vitro for 7 days and then incubated for 24 h with 100-nmol MTX. Forty-eight and 72 h following the exposure to MTX, the treated and control cultures were harvested. Specimens and cultures were examined by light and scanning electron microscopy. In the in vivo cohort, no differences were observed between MTX and control groups. However, in MTX-treated cell culture, ciliated cells demonstrated partially adherent cilia in about 30% of the cells. The effect of MTX observed only in vitro treated cells may be due to the rapid proliferation of epithelial cells in culture which does not represent the physiological role of the oviduct in vivo.

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Section: Discussionmentioning

confidence: 99%

Effects of Methotrexate on Rabbit Oviducts and on Cell Cultures of Bovine Oviduct Epithelium

Tepper

Nahum²,

Rahamim³

et al. 1992

Gynecol Obstet Invest

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“…Direct comparisons between different experiments become possible and such preparations could afford a means of extending current studies of placental function to the cellular level. 1978;Cotte et al 1980). 1Y78;Stromberg et al 1978: Niazi et al 1981 although cells have been maintained in static culture for 6-22 months (Paul ef al.…”

Section: Discussionmentioning

confidence: 99%

Long‐term culture of human trophoblast cells

Morgan

Toothill

Landon

1985

BJOG

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A technique is presented for the preparation of cultures of replicating human trophoblast cells from term placentas. The adherent cells obtained were very slow growing (doubling time 12.5 days) a s measured b y the rate of increase in cell protein, 1'4Cl-leucine uptake and cell number. Cells from individual placentas have been maintained in continuous culture for up t o 1 year (10-12 passages) and have been successfully recultured after storage in liquid nitrogen. Cultured cells showed positive immunofluorescent staining for human placental lactogen, human chorionic gonadotrophin, transferrin and type IV collagen. The adenine nucleotide content indicated that energetically the cells were in balance even after prolonged culture.

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“…This is retained for a longer time (Cotte et al, 1980;Lueck and Aladjem, 1980;Loke, 1983;Nelson et al, 1986;Kliman et al, 1990;Aboagye-Mathiesen et al, 1996;Rong-Hao et al, 1996). This is retained for a longer time (Cotte et al, 1980;Lueck and Aladjem, 1980;Loke, 1983;Nelson et al, 1986;Kliman et al, 1990;Aboagye-Mathiesen et al, 1996;Rong-Hao et al, 1996).…”

Section: In Vitro Assaymentioning

confidence: 99%