Effects of Methotrexate on Rabbit Oviducts and on Cell Cultures of Bovine Oviduct Epithelium

Tepper, R.; Nahum, Ravit; Rahamim, Esra; Kapolovitz, Yuri; Segal, Yehoshua; Karib, Noam; Beyth, Yoram

doi:10.1159/000294850

Cited by 3 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extrauterine pregnancies would not be such an unusual finding if we were to perform regular necropsies on animals. Different species have successfully been used as animal models in ectopic pregnancy studies, mainly for the evaluation of new treatments and surgical techniques (Nelson et al 1986, Tepper et al 1992, Popp et al 1993) that have later been used in human beings. However, it is necessary to clarify several aspects such as the aetiology of this condition and the implicated factors.…”

Section: Expectant Managementmentioning

confidence: 99%

Ectopic pregnancy in animals and humans

Corpa¹

2006

Reproduction

View full text Add to dashboard Cite

Ectopic pregnancy denotes a pregnancy occurring elsewhere than in the cavity of the uterus. This pathology has been recognised for years and it causes numerous maternal deaths during the first trimester of pregnancy. While this condition is wellknown in humans, it is rarely diagnosed in animals. However, the causes and mechanisms leading to an ectopic implantation of the ovum are not always clearly defined in humans or animals. Two types of ectopic pregnancy are mainly recognized: (1) tubal pregnancy occurs when an oocyte is fertilized and then remains in the oviduct and (2) abdominal pregnancy occurs when the gestation develops in the peritoneal cavity. The latter may be subdivided into two subtypes: the primary form, when a fertilized oocyte enters the peritoneal cavity and becomes attached to the mesentery or abdominal viscera, and the secondary form, which follows the rupture of an oviduct or the uterus after the fetus has been implanted, and the fetus is expelled into the peritoneal cavity. Cornual, ovarian and cervical ectopic locations are less frequent. Several differences exist in ectopic pregnancies between human beings and animal species. While abdominal pregnancy has been described in both human and animal species, tubal ectopic pregnancies would appear to be restricted to primates. Other than anecdotal cases, this pathological condition does not occur in laboratory, domestic or farm animals. Several factors are described as being the cause of these differences.

show abstract

Section: Expectant Managementmentioning

confidence: 99%

Ectopic pregnancy in animals and humans

Corpa¹

2006

Reproduction

View full text Add to dashboard Cite

show abstract

“…A model for tubal pregnancy has been established by the arrest of embryos in the Fallopian tubes using appropriately positioned ligatures. Such artificially-retained embryos may progress to the blastocyst stage in the Fallopian tube, but development thereafter is severely compromised and degeneration soon follows (Jacobson et al, 1987;Kooi et al 1992;Lecuru et al, 1992;Popp et al, 1993;Tepper et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Methotrexate and fallopian tubes in ectopic pregnancy

Glišić¹,

Radunović²,

Atanacković³

2006

Acta vet. (Beogr.)

View full text Add to dashboard Cite

The condition of ectopic pregnancy is presented from different points of view, bringing out physiological explanations for its occurrence in primates and the striking absence in other mammals. Ectopic pregnancies have been described in several animal species such as cats, sheep, cows, horses gerenuks, pigs, hamsters, rats, rabbits, marmosets, baboons, squirrel monkeys, owl monkeys and rhesus macaque. Methotrexate (MTX) has anticancer antibacterial, antifungal, antiparasitic, immunosuppressive, antimitotic embryotoxic and teratogenic effects. Methotrexate has species-specific effects. The purpose of this study was to investigate the medical treatment outcome of ectopic pregnancy by local administration of methotrexate and the adverse effects of increasing levels of MTX on the epithelium of the Fallopian tube as viewed by light microscopy. We treated patients with local and systemic regimen of methotrexate. Local applications were performed by direct injection of 0,5 mg/kg or 1 mg/kg methotrexate into the amniotic sac under sonographic control. Fallopian tubes were removed in patients with methotrexate treatment failure. The histological features we observed in the Fallopian tube were similar in all groups. In particular, histological findings showed necrosis and inflammatory infiltrate that was not quantitatively parallel to the increase in MTX dose. Fallopian tubes in human pregnancy could be resistant to the influence of MTX

show abstract

Medical therapy of ectopic gestation: contemporary paradigms

1996

View full text Add to dashboard Cite

There has been an increase in the incidence of ectopic pregnancy to epidemic proportions in many developed countries in the past two decades. In 1989, ectopic pregnancy accounted for 1.6% of all pregnancies in the United States of America. This trend may be explained by an increase in the incidence of established risk factors as well as a dramatic improvement in diagnostic tools and advances in reproductive technology. Technologic advances such as highly sensitive radioimmunoassays for the β-subunit of human chorionic gonadotropin (βhCG), the development of high-resolution ultrasound and a heightened physician awareness have revolutionized the clinical management of ectopic pregnancy, leading to earlier and more consistent diagnoses and consequently reduced maternal mortality rates. 1,3–13 Unfortunately, ectopic pregnancy is still associated with a significant morbidity and mortality and contributes substantially to health-care costs. For example ectopic pregnancy complications accounted for 13% of all pregnancy-related deaths and were the leading cause of maternal mortality in African- American women in 1989. Financially, an estimated 1.1 billion dollars was spent in the United States of America alone for the management of ectopic pregnancy in 1990.

show abstract

Effects of Methotrexate on Rabbit Oviducts and on Cell Cultures of Bovine Oviduct Epithelium

Cited by 3 publications

References 7 publications

Ectopic pregnancy in animals and humans

Ectopic pregnancy in animals and humans

Methotrexate and fallopian tubes in ectopic pregnancy

Medical therapy of ectopic gestation: contemporary paradigms

Contact Info

Product

Resources

About