Preparation of heparin-immobilized PVA and its adsorption for low-density lipoprotein from hyperlipemia plasma

Abstract

In this study, heparin was covalently coupled by glutaraldehyde to Poly(vinyl alcohol) [PVA] in solid-liquid two-phase reaction system by two-step synthesis method to prepare a LDL-selective adsorbent. The parameters (the material ratio, reaction time and dosage of catalyzer) were investigated to evaluate their effect upon the immobilized amount of heparin onto the surface of PVA, IR was used to verify the covalent immobilization result and the heparin-modified PVA was also undergone the evaluation of its adsorption capability for low-density lipoprotein from hyperlipemia plasma, and its hemocompatibility was preliminarily evaluated by platelet adhesion test. Results showed: (1) under optimized reaction conditions the highest immobilization amount of heparin onto PVA surface within the experiments of this study has been obtained; (2) the optimized reaction conditions were: (i) at the refluxing temperature 78 degrees C; (ii) the material ratio of "PVA(g): 50% glutaraldehyde (ml)" was about "1:3"; (iii) the reaction time was about 5 h; and (iv) the amount of catalyzer (concentrated HCL) was about 1% of the 50% glutaraldehyde; (3) within the experiments of this study the highest immobilization amount would be up to 25 microg heparin on the surface of per g PVA granules; (4) the heparin-modified PVA granules showed significant adsorption for LDL under faintly alkaline environment (pH=7.2-9.5) ; (5) The result of platelet adhesion test showed no platelet adhered to its surface. Therefore, immobilization of heparin onto the surface of a support is one approach to prepare a kind of LDL adsorbent for blood purification.

Covalent Heparin Modification of a Polysulfone Flat Sheet Membrane for Selective Removal of Low‐Density Lipoproteins: A Simple and Versatile Method

“…After the immobilization of heparin, the sulfate and nitrogen contents on the PSf-Hep surface decreased from 2.25 to 1.87% and from 8.24 to 3.11%, respectively, compared to those on the PSfPlasma surface, due to the relatively lower sulfate and nitrogen content of heparin molecules covering the PSf surface layer. [25,28] These results confirm the fact that the PSf membrane can be easily heparinized through the plasma activation process.…”

“…New absorption peaks of PSf-Hep at 1 648 and 1 542 cm À1 arise from the carbonyl group (C¼O) vibration of amides of heparin molecules, while the peak at 1 738 cm À1 arises from the C¼O vibration of carboxylic acids, compared with the PSf and PSfPlasma. [25,28] Therefore, heparin had apparently been successfully immobilized onto the PSf surface. However, the characteristic absorption range of the amine groups on the PSf-Plasma overlapped with one of hydroxyl groups, thus a further chemical structure analysis is required for confirmation.…”

Multilayer Cucurbit[6]uril‐Based Magnetic Nanoparticles Prepared by Host–Guest Interaction: Remarkable Adsorbent for Low Density Lipoprotein Removal from Plasma

“…In addition, ac omparison of MNPs-(HA-DAH 5 / HA-CB[6] 5 )w ith some reported adsorbentsf or LDL removal ( Table S3 in the SupportingI nformation) revealed that the prepared material provided comparable adsorption capacity and rapidness.I ti si nteresting to note that the present adsorbent exhibiteds uperior recyclability than previously reported materials. [6][7][8][9][31][32][33][34][35][36][37]…”

Targeting regulation of the tumour microenvironment induces apoptosis of breast cancer cells by an affinity hemoperfusion adsorbent

“…Heparin is a highly sulphated linear polysaccharide belonging to the family of glycosaminoglycans and has been widely used as an anticoagulant agent. In the field of adsorbent-based hemoperfusion, heparin is always introduced to functionalize polymers in order to improve the blood compatibility of adsorbent, or used as a good ligand for the removal of certain toxins, including endotoxin and low-density lipoprotein (LDL) [13]. However, the role of PVA-H in targeting regulation of TME using hemoperfusion column is not reported.…”