Multilayer Cucurbit[6]uril‐Based Magnetic Nanoparticles Prepared by Host–Guest Interaction: Remarkable Adsorbent for Low Density Lipoprotein Removal from Plasma

Abstract

Efficient removal of low density lipoprotein (LDL) is a key challenge due to its high level in plasma as a primary risk factor in the pathogenesis of atherosclerotic cardiovascular disease. In this work, a facile synthesis strategy based on host-guest interactions was developed to prepare multilayer cucurbit[6]uril-based magnetic nanoparticles, MNPs-(HA-DAH /HA-CB[6] ). The compound was employed as a blood purification material for the removal of LDL from plasma because it had good blood compatibility and could be easily separated with an external magnet. The efficient removal of LDL was attributed to the electrostatic interactions between the positive charged apoB-100 domain of LDL and the negative charged adsorbent. Moreover, the prepared material exhibited high recyclability and could release LDL in physiological saline for recyclable use. MNPs-(HA-DAH /HA-CB[6] ) offered promising perspectives and broad applications in extracorporeal treatment for the removal of LDL.

Design of Two-Dimensional Layered Double Hydroxide Nanosheets Embedded with Fe3O4 for Highly Selective Enrichment and Isotope Labeling of Phosphopeptides

“…It is generally known that excellent magnetic response characteristics of magnetic nanoparticles are essential for successful separation and enrichment . Vibrating sample magnetometer (VSM) analysis was performed to study the magnetic characteristics of Fe 3 O 4 and Fe 3 O 4 -LDH composites (Figure S5B).…”

“…Hemolysis ratio (HR) was investigated to evaluate the blood compatibility properties of Fe 3 O 4 -LDH 2 . HR can be examined as eq : where D t is the absorbance of the sample, D nc and D pc are absorbances of the negative (normal saline) and positive controls (water). HR of Fe 3 O 4 -LDH 2 (2.16%) was lower than the clinic limit (5%), illustrating that Fe 3 O 4 -LDH 2 met the requirement of biomaterials.…”

Aptamer‐Functionalized Magnetic Conjugated Organic Framework for Selective Extraction of Traces of Hydroxylated Polychlorinated Biphenyls in Human Serum

“…Fe 3 O 4 @SiO 2 (1.0 g) was dispersed in ethanol (50 mL) and APTES (1.0 mL) was added. Subsequently, the suspension was stirred at 25 °C for 1 h to obtain Fe 3 O 4 @SiO 2 @NH 2 …”

“…Subsequently,t he suspension was stirred at 25 8Cf or 1hto obtain Fe 3 O 4 @SiO 2 @NH 2 . [15,42] TMC (1.325 g) was dissolved in EA (30 mL), and Fe 3 O 4 @SiO 2 @NH 2 (1.0 g) was added to the solution with stirring at 0 8Cf or 1h.A mixture of TMC (1.06 g) and PPD (0.32 g) in EA (30 mL) was added to the resulting solution with stirring at 0 8Cf or 1h.F inally, Fe 3 O 4 @COF-COOH was obtained after reaction at 25 8Cf or 24 h, washed with H 2 Oa nd ethanol, and dried in av acuum oven overnight at 60 8C. [38] Fe 3 O 4 @COF-COOH (1.0 g) was dispersed in PBS (50 mL, pH 7.4) containing EDC (0.8 g) and NHS (1.2 g).…”

Biomembrane‐mimetic hemoperfusion adsorbent for efficient removal of low‐density lipoprotein from hyperlipemia blood

“…To date, based on the positively charged apolipoprotein B-100 (apoB-100), various anionic adsorbents have been exploited in the development of LDL hemoperfusion technique, such as dextran sulfate cellulose, 7 polyacrylate modified polyacrylamide beads, 8 polysaccharides modified resins, [9][10][11][12][13][14] sulfonated carbon-based materials 15 and porous polymer beads small negatively charged groups (sulfonate, phosphate and carboxyl groups). [16][17][18][19] However, the traditional LDL adsorbents with good adsorption capacities always demonstrate inferior blood compatibility which restricts their efficiency for clinical use.…”