Preparation of Frozen Sections for Analysis

Bratthauer, Gary L.

doi:10.1007/978-1-59745-324-0_10

Cited by 7 publications

(4 citation statements)

References 5 publications

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“…Frozen sections were prepared using a Cryostar NX50 Cryostat according to the reported procedure. 36 CLSM imaging was performed on an Olympus FV1000 confocal scanning system with a 60× and 20× oil-immersion objective lens for cells and frozen sections, respectively. Red channel: 700 ± 50 nm, λ ex = 633 nm.…”

Section: Methodsmentioning

confidence: 99%

Deformylation reaction-based probe forin vivoimaging of HOCl

et al. 2018

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Section: Methodsmentioning

confidence: 99%

Deformylation reaction-based probe forin vivoimaging of HOCl

et al. 2018

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“…They are then placed on dry ice or floated in liquid nitrogen on an aluminum foil weighing boat to accomplish rapid freezing. An alternative approach is to freeze cryo-preserved lung tissue in isopentane (2-methylbutane) that has been precooled in liquid nitrogen (Erickson et al 2011; Bratthauer 2010). Frozen specimens are wrapped in clean aluminum foil and placed inside sealable plastic bags for storage at −80°C until sectioning (Mercer et al 2008).…”

Section: Features Measurements and Practical Aspects Of Ards Research In Micementioning

confidence: 99%

Mouse Models of Acute Respiratory Distress Syndrome

Aeffner¹,

2015

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Acute respiratory distress syndrome (ARDS) is a severe pulmonary reaction requiring hospitalization, which is incited by many causes, including bacterial and viral pneumonia as well as near drowning, aspiration of gastric contents, pancreatitis, intravenous drug use, and abdominal trauma. In humans, ARDS is very well defined by a list of clinical parameters. However, until recently no consensus was available regarding the criteria of ARDS that should be evident in an experimental animal model. This lack was rectified by a 2011 workshop report by the American Thoracic Society, which defined the main features proposed to delineate the presence of ARDS in laboratory animals. These should include histological changes in parenchymal tissue, altered integrity of the alveolar capillary barrier, inflammation, and abnormal pulmonary function. Murine ARDS models typically are defined by such features as pulmonary edema and leukocyte infiltration in cytological preparations of bronchoalveolar lavage fluid and/or lung sections. Common pathophysiological indicators of ARDS in mice include impaired pulmonary gas exchange and histological evidence of inflammatory infiltrates into the lung. Thus, morphological endpoints remain a vital component of data sets assembled from animal ARDS models.

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“…Comparing with simple freeze agents, the freezing by substitution method has the advantage of reducing cell membranes disruption and accelerating the decrease of tissue temperature (2,18) . Isopentane is the most commonly used substance in freezing by substitution.…”

Section: Cryoprotection and Freezingmentioning

confidence: 99%

Influence of external factors on the preservation of human nervous tissue for histological studies: review article

Santos¹,

Del‐Bel²,

Pittella³

et al. 2014

Jornal Brasileiro De Patologia E Medicina Laboratorial

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Neuropathological studies are crucial for the new knowledge on pathophysiology and treatment of neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease. The postmortem brain tissue processing method directly impacts on both the appropriate integrity and the biomolecules detection by different histological and molecular biology techniques. In this review we will discuss topics on the influence of some external factors on the preservation of the brain tissue for histological studies (histochemistry and immunohistochemistry), such as factors either prior or after the death, and the chosen method for the preservation of nervous tissue. By means of a specific example, we propose a strict record of various conditions involved in the method of preservation of nervous tissue, and its correlation with variables that measure the quality of the histological sample as markers of preservation of biological material for further studies.

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Preparation of Frozen Sections for Analysis

Cited by 7 publications

References 5 publications

Deformylation reaction-based probe forin vivoimaging of HOCl

Deformylation reaction-based probe forin vivoimaging of HOCl

Mouse Models of Acute Respiratory Distress Syndrome

Influence of external factors on the preservation of human nervous tissue for histological studies: review article

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