Preparation of flat gold terraces for protein chip developments

Élie-Caille, Céline; Rauch, Jean-Yves; Rouleau, Alain; Boireau, Wilfrid

doi:10.1049/mnl.2009.0008

Cited by 6 publications

(5 citation statements)

References 12 publications

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“…Cytochrome b5 is reduced with DTT to form monomers and to immobilize the protein onto the Au nanoparticle substrate. Reduced cytochrome b5 is able to form gold-thiol bonds, leading to direct self-assembly onto the gold surface by chemisorption [43].…”

Section: Cytochrome B5mentioning

confidence: 99%

Homogeneous large-scale crystalline nanoparticle-covered substrate with high SERS performance

et al. 2015

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This article details the surface-enhanced Raman scattering (SERS) performance of plasmonic substrates fabricated by a physical metal evaporation technique that uses no precursor or intermediate coating. We outline a cost-effective nanofabrication protocol that uses common laboratory equipment to produce homogeneously covered crystalline nanoparticle substrates. Our fabrication yields a homogeneous SERS response over the whole surface. The platform is tested with methylene blue diluted at various concentrations to estimate the sensitivity, homogeneity, and reproducibility of the process. The capacity of the substrates is also confirmed with spectroscopic investigations of human microsomal cytochrome b5.

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Section: Cytochrome B5mentioning

confidence: 99%

Homogeneous large-scale crystalline nanoparticle-covered substrate with high SERS performance

et al. 2015

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“…Previous studies have focused on fabricating ultraflat gold contacts for applications ranging from plasmonics to protein chip development . Although gold is a popular metal contact of choice owing to its chemical inertness and biocompatibility, it is still subject to numerous topological imperfections.…”

mentioning

confidence: 99%

“…Previous studies have focused on fabricating ultraflat gold contacts for applications ranging from plasmonics 8 to protein chip development. 9 Although gold is a popular metal contact of choice owing to its chemical inertness and biocompatibility, it is still subject to numerous topological imperfections. Earlier reports have investigated the influence of the substrate material, 10 temperature, 11 deposition rate, 12 and chamber pressure during evaporation 11,13 on the grain size, in-grain root-mean-square roughness (rms), and surface defects.…”

mentioning

confidence: 99%

Nanoscale Origin of Defects at Metal/Molecule Engineered Interfaces

et al. 2013

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The control and repair of defects at metal/molecule interfaces is central to the realization of molecular electronic circuits with reproducible performance. The fundamental mechanism governing defect (pore) evolution on mica-supported metal surfaces, its propagation in self-assembled molecular layers, and its implications for molecular junction devices are discussed. Pore eradication by replacing mica with halide platforms coupled with elevated substrate temperature during metal deposition yields exceptionally ultraflat metal landscapes. In situ scanning tunneling microscopy further substantiates molecular locking at defect sites and upon defect healing; the emergence of a closely packed 2-D molecular architecture is demonstrated with nanometer-scale spatial resolution in liquids.

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“…Therefore, the kinetic and thermodynamic profiles can be different from the ones observed on flat gold surfaces . However, the roughness of the surface of the gold layer constituting the SPR chip makes it comparable to a continuous monolayer of gold grains exposing the top part of GNPs of about 30 nm in diameter . In this way, SPR allows easy screening of the trends in reactivity and therefore on the best conditions to employ for the grafting of thiolated molecules on the gold surface.…”

Section: Introductionmentioning

confidence: 99%

“…45 However, the roughness of the surface of the gold layer constituting the SPR chip makes it comparable to a continuous monolayer of gold grains exposing the top part of GNPs of about 30 nm in diameter. 46 In this way, SPR allows easy screening of the trends in reactivity and therefore on the best conditions to employ for the grafting of thiolated molecules on the gold surface. This faster method can save time and materials compared to tests carried out on GNP batches.…”

Section: ■ Introductionmentioning

confidence: 99%

Inferring the Interfacial Reactivity of Gold Nanoparticles by Surface Plasmon Resonance Measurements

Romain,

Roman,

Saviot

et al. 2023

Langmuir

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Gold nanoparticles (GNPs) require a functionalization step in most cases to be suitable for applications. Optimizing this step in order to maintain both the stability and the plasmonic properties of the GNPs is a demanding process. Indeed, multiple analyses are required to get sufficient information on the grafting rate and the stability of the obtained suspension, leading to material and time waste. In this study, we propose to investigate ligand reactivity on a gold surface with surface plasmon resonance (SPR) measurements as a way to simulate the reactivity in GNP suspensions. We consider two thiolated ligands in this work: thioglycolic acid (TA) and 6-mercaptohexanoic acid (MHA). These thiols are grafted using different conditions on GNPs (monitored by optical absorption) and on a gold surface (monitored by SPR) and the grafting efficiency and stability are compared. The same conclusions are reached in both cases regarding the best protocol to implement, namely, the thiol molecules should be introduced in a water solution at a low concentration. This demonstrates the suitability of SPR to predict the reactivity on a GNP surface.

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Preparation of flat gold terraces for protein chip developments

Cited by 6 publications

References 12 publications

Homogeneous large-scale crystalline nanoparticle-covered substrate with high SERS performance

Homogeneous large-scale crystalline nanoparticle-covered substrate with high SERS performance

Nanoscale Origin of Defects at Metal/Molecule Engineered Interfaces

Inferring the Interfacial Reactivity of Gold Nanoparticles by Surface Plasmon Resonance Measurements

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