Preparation of fatty acid methyl esters by direct transesterification of lipids with aluminium chloride—methanol

Segura, Ramón

doi:10.1016/s0021-9673(01)84658-6

Cited by 21 publications

(9 citation statements)

References 45 publications

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“…The plasma fatty acid composition was determined by gas-liquid chromatography, on a capillary column, after methylation of the corresponding compounds (21). In the different tests (laboratory exercise test, aerobic and anaerobic field test), blood samples were obtained to evaluate metabolic responses with levels of lactate (Vario photometer, Diaglobal GmbH, Berlin, Gemany), uric acid and glucose (Reflotron, Roche diagnostics GmbH, Manhein, Germany).…”

Section: Methodsmentioning

confidence: 99%

The effect of supplementation wit n-3 fatty acids on the physical performance in subjects with spinal cord injury

Javiere

Vidal

Segura

et al. 2006

J. Physiol. Biochem.

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A global physical evaluation was performed in 21 males with spinal cord injury (SCI), at the beginning and at three and six months of omega-3 fatty acid (FA) supplementation. A significant increase in the proportion of eicosapentaenoic acid and docosahexanoic acid in plasma was observed in response to the supplementation (p<0.05). After six months of FA supplementation, strength endurance time increased from 127.7+/-19.0 s to 215.2+/-45.6 s in the right arm, and from 139+/-27.6 s to 237.7+/-48.7 s, in the left arm. The time to perform 20 repetitions of 70% maximum workload showed a reduction of 41% between the first and the third test. The time taken to cover a 90 meter long track, with a 6% slope, was reduced from 66.9+/-8.0 s to 59.3+/-6.7 s, at the end of the study (p<0.05). In conclusion, omega-3 FA supplementation could contribute to improve the functional capabilities in SCI subjects.

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Section: Methodsmentioning

confidence: 99%

The effect of supplementation wit n-3 fatty acids on the physical performance in subjects with spinal cord injury

Javiere

Vidal

Segura

et al. 2006

J. Physiol. Biochem.

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“…Boron trifluoride in methanol (12-14%) is the most often used for transesterification of RBC lipids [26]. Several other reagents used for trans-esterification of human and plant samples include acetyl chloride [17,26,36,37] and aluminum chloride [26,38].…”

Section: Methylation Proceduresmentioning

confidence: 99%

A Rapid and Simple Method for Fatty Acid Profiling and Determination of ω-3 Index in Red Blood Cells

Akinyemi¹,

Bruckner²,

Johnson³

et al. 2017

TONUTRJ

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Fatty acid profiling has become a very useful and effective tool in the diagnosis, prevention and treatment of several diseases with cardiovascular disease being particularly important. In order to arrive at accurate conclusions that would help promote the health of individuals plagued by such diseases, not only excellent laboratory methods are required, but also very important monitoring responses to treatment. Improvements in methods of fatty acid profiling in biological systems regarding safety of extraction, precision and time for analysis are valuable. The ω-3 index (a measure of the amount of eicosapentaenoic acid, EPA, and docosahexaenoic acid, DHA, in Red Blood Cell membranes expressed as the percent of total fatty acids) is of growing interest because it has been reported to provide prognostic information regarding the risk for heart diseases. Sodium methoxide has been widely used for the determination of ω -3 fatty acids in food samples. This study demonstrates that sodium methoxide can be used effectively in RBC fatty acid profiling and determination of the ω-3 index. Briefly, the fatty acid profiles and ω-3 index of red blood cell samples were analyzed and results compared using three different methods: a two-step extraction and methylation method described by Hara and Radin, a single step extraction and methylation method described by Harris et al. and the sodium methoxide method.Our results revealed that there were no statistically significant differences (p<0.05) between the three methods for the representative fatty acids, [16:0 (p = 0.10), 18:0 (p=0.40), 18:1(ω9) (p = 0.29), 18:2(ω6) (p = 0.95), 18:3(ω3) (p = 0.50), 20:5(ω3) (p=0.56), 22:6(ω3) (p = 0.06)] and ω-3 index (p = 0.11) except for 20:4(ω6), (P = 0.02). In conclusion, we show that sodium methoxide can be used effectively in a one-step extraction and methylation procedure for high throughput analysis of fatty acids in red blood cell membranes. It is rapid (10 minute extraction and methylation), simple, safer than and as accurate as other commonly reported methods.

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“…The rest of the sample was centrifuged at 1815 rpm for 20 min to separate the blood plasma which was then used to determine the content and the proportion of the different fatty acids. These were determined by analyzing the methyl-esters of the corresponding compounds, obtained by a process of direct trans-sterification 10 and separated by means of gas-liquid chromatography, using a chromatograph (model HP 6890 serie GC, Hewlett-Packard, Wilminston, USA) equipped with a capillary column and a flame ionization detector.…”

Section: Analysis Of Blood Samplesmentioning

confidence: 99%

Continual supplementation with n-3 fatty acids does not modify plasma lipid profile in spinal cord injury patients

et al. 2005

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Preparation of fatty acid methyl esters by direct transesterification of lipids with aluminium chloride—methanol

Cited by 21 publications

References 45 publications

The effect of supplementation wit n-3 fatty acids on the physical performance in subjects with spinal cord injury

The effect of supplementation wit n-3 fatty acids on the physical performance in subjects with spinal cord injury

A Rapid and Simple Method for Fatty Acid Profiling and Determination of ω-3 Index in Red Blood Cells

Continual supplementation with n-3 fatty acids does not modify plasma lipid profile in spinal cord injury patients

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