A Rapid and Simple Method for Fatty Acid Profiling and Determination of ω-3 Index in Red Blood Cells

Akinyemi, Olufunmilola; Bruckner, Géza; Johnson, J D; Lennie, Terry A.; Hildebrand, David F.

doi:10.2174/1874288201711010017

Cited by 4 publications

(4 citation statements)

References 43 publications

(57 reference statements)

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“…As a caveat, Wang and Quinn report that tocopherol preferentially partitions into PC-rich lipid domains rather than phosphoethanolamine, irrespective of the unsaturation . This headgroup dependence cannot go unnoticed when discussing vitamin E as a biological antioxidant because it has been shown that PUFA chains are more likely to be attached to a phosphoethanolamine headgroup in red blood cells. − Here, we did not investigate different lipid headgroups; rather, by maintaining a constant headgroup, our data clearly illustrate a reduction in αToc volume, indicating a favorable interaction that directly correlates to chain unsaturation.…”

Section: Discussionmentioning

confidence: 74%

Partial Volumes of Phosphatidylcholines and Vitamin E: α-Tocopherol Prefers Disordered Membranes

et al. 2022

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Despite its discovery over 95 years ago, the biological and nutritional roles of vitamin E remain subjects of much controversy. Though it is known to possess antioxidant properties, recent assertions have implied that vitamin E may not be limited to this function in living systems. Through densitometry measurements and small-angle X-ray scattering we observe favorable interactions between α-tocopherol and unsaturated phospholipids, with more favorable interactions correlating to an increase in lipid chain unsaturation. Our data provide evidence that vitamin E may preferentially associate with oxygen sensitive lipidsan association that is considered innate for a viable membrane antioxidant.

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Section: Discussionmentioning

confidence: 74%

Partial Volumes of Phosphatidylcholines and Vitamin E: α-Tocopherol Prefers Disordered Membranes

et al. 2022

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“…Association between microbiome abundance at the species (top) or genus (bottom) level and nutrient intake concentration averaged over the 3 days prior to the biological collection. Three days has previously been shown to be adequate for dietary evaluation in relation to the microbiome 24 .…”

Section: Discussionmentioning

confidence: 99%

“…Total serum lipids were determined colorimetrically on the Ace Alera (Alpha Wasserman, Caldwell, NJ) using a kit from WAKO Diagnostics (Mountain View, CA); within-and between-assay CVs were 2.8 and 4.5%, respectively). Short and long-chain fatty acid composition of serum 23 , red blood cells 24 , and fecal samples were determined by a send-out laboratory at Duke University (Durham, NC) using quadrupole mass spectrometry (Waters Xevo TQ-S, Milford, MA) based on the work of Han, et al 25 , and it was implemented in the Duke Proteomics and Metabolomics Core Facility (DPMCF).…”

mentioning

confidence: 99%

Impact of diet on human nutrition, immune response, gut microbiome, and cognition in an isolated and confined mission environment

Douglas

DeKerlegand

Dlouhy

et al. 2022

Sci Rep

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Long-duration spaceflight impacts human physiology, including well documented immune system dysregulation. The space food system has the potential to serve as a countermeasure to maladaptive physiological changes during spaceflight. However, the relationship between dietary requirements, the food system, and spaceflight adaptation requires further investigation to adequately define countermeasures and prioritize resources on future spaceflight missions. We evaluated the impact of an enhanced spaceflight diet, with increased quantity and variety of fruits, vegetables, fish, and other foods rich in flavonoids and omega-3 fatty acids, compared to a standard spaceflight diet on multiple health and performance outcomes in 16 subjects over four 45-day closed chamber missions in the NASA Human Exploration Research Analog (HERA). Subjects consuming the enhanced spaceflight diet had lower cholesterol levels, lower stress (i.e. cortisol levels), better cognitive speed, accuracy, and attention, and a more stable microbiome and metatranscriptome than subjects consuming the standard diet. Although no substantial changes were observed in the immune response, there were also no immune challenges, such as illness or infection, so the full benefits of the diet may not have been apparent in these analog missions. These results indicate that a spaceflight diet rich in fruits, vegetables, and omega-3 fatty acids produces significant health and performance benefits even over short durations. Further investigation is required to fully develop dietary countermeasures to physiological decrements observed during spaceflight. These results will have implications for food resource prioritization on spaceflight missions.

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“…1 Scalp skin surface (Koch et al, 1982); 2 Sole skin epidermis (Ansari et al, 1970);3 Normal (N), uninvolved (PU), and involved (PI) psoriatic forearm skin (Chapkin et al, 1986); 4 Erythrocyte membrane (Akinyemi et al, 2017); 5 Plasma lipids (Abdelmagid et al, 2015).…”

Section: Lipids Of Healthy Skinmentioning

confidence: 99%

The Enigma of Bioactivity and Toxicity of Botanical Oils for Skin Care

2020

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Botanical oils have a long history of traditional use and are routinely applied to skin care. The focus of this review is to contrast the functionality of skin oils versus the differential biological and toxicological effects of major plant oils, and to correlate them to their compositional changes. In total, over 70 vegetable oils were clustered according to their lipid composition to promote awareness of health practitioners and botanical product manufacturers for the safety and efficacy of oil-based interventions based on their fatty acid profiles. Since multiple skin disorders result in depletion or disturbance of skin lipids, a tailored mixture of multiple botanical oils to simultaneously maintain natural skin-barrier function, promote repair and regeneration of wounded tissues, and achieve corrective modulation of immune disorders may be required. As bioactive constituents of botanical oils enter the human body by oral or topical application and often accumulate in measurable blood concentrations, there is also a critical need for monitoring their hazardous effects to reduce the possible over-added toxicity and promote maximal normal tissue sparing. The review also provides a useful tool to improve efficacy and functionality of fatty acid profiles in cosmetic applications.

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A Rapid and Simple Method for Fatty Acid Profiling and Determination of ω-3 Index in Red Blood Cells

Cited by 4 publications

References 43 publications

Partial Volumes of Phosphatidylcholines and Vitamin E: α-Tocopherol Prefers Disordered Membranes

Partial Volumes of Phosphatidylcholines and Vitamin E: α-Tocopherol Prefers Disordered Membranes

Impact of diet on human nutrition, immune response, gut microbiome, and cognition in an isolated and confined mission environment

The Enigma of Bioactivity and Toxicity of Botanical Oils for Skin Care

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