Preparation of enteric-coated microspheres of Mycoplasma hyopneumoniae vaccine with cellulose acetate phthalate: I. Formation condition and micromeritic properties

Lin, Shan‐Yang; Tzan, Y. L.; Lee, C. J.; Weng, Chung-Nan

doi:10.3109/02652049109069558

Cited by 27 publications

(4 citation statements)

References 14 publications

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“…1±1.9, and from 97.8±1.6 to 73.1±2.1, for F3 and F6 respectively (table 5). The intact and smooth microspheres will be formed at a low surfactant concentration, but as the concentration increased, the microspheres become more brittle and much drug will be lost during washing [19]. The addition of Cbp940 had a non-significant effect on the drug entrapment.…”

Section: Entrapment Efficiencymentioning

confidence: 99%

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Tioconazole Loaded Mucoadhesive Microspheres for the Treatment of Vaginal Candidiasis

Sabry

2018

Int J Pharm Pharm Sci

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Objective: The principal objective of this research work was to formulate tioconazole into mucoadhesive microspheres which were inserted into the vagina for sustaining the anti-fungal activity and protecting the liver and the kidney from the harmful drug side effects.Methods: Microspheres were prepared by the emulsion solvent evaporation method, using different ratios of the drug with either ethyl cellulose N14 or hydroxypropylmethylcellulose K100M (HPMC K100M) as mucoadhesive polymers. The formulated microspheres were evaluated for the particle size, entrapment efficiency, yield percentage, mucoadhesion strength, swelling percentage, pH, in vitro release of the drug and finally in vitro antifungal activity.Results: The optimized formulae were F3 and F6, which showed drug entrapment of 88.4±2.5 % and 97.8±1.6 %, yield percentage of 85.0±2.3% and 95.2±1.2%, mucoadhesion strength of 31.5±1.6 and 38.1±1.6 *103 dyne/cm2, swelling percentages of 143.1±1.3 and 154.2±0.8, pH of 4.7±0.1 and 4.6±0.5, and particle size of 400±3.5 µm and435±7. 8 µm respectively. F3 and F6 released about 72.8%±3.2 and 58.4%±2.7 of the drug after 8 h respectively. F3 and F6 showed a significant anti-fungal activity.Conclusion: Ethyl cellulose and hydroxyl propyl methylcellulose mucoadhesive microspheres are considered a good way to increase the duration of the anti-fungal activity of tioconazole with minimum side effects.

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Section: Entrapment Efficiencymentioning

confidence: 99%

“…9 and 10 show that an increase in the surfactant concentration to 1.5% increased the cumulative release to 78.1±and 87.6±0.5, for F3 and F6 respectively. The increased surfactant concentration gave porous microspheres which resulted in higher release percentages [19,32].…”

Section: In Vitro Release Studymentioning

confidence: 99%

Tioconazole Loaded Mucoadhesive Microspheres for the Treatment of Vaginal Candidiasis

Sabry

2018

Int J Pharm Pharm Sci

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“…2,3 The pharmaceutical industry used various approaches for the development of colon-targeted drug delivery systems, one of them is: embedding in biodegradable matrices in which the matrices of polysaccharides are assumed to remain intact in the physiological environment of stomach and small intestine but once they reach in the colon, they are acted upon by the bacterial polysaccharidases and results in the degradation of the matrices. 4 In this sense, the use of diverse polysaccharides as pectin and their salts, also amylosa, 4 dextrin, guar, 5 hydroxylpropylmethylcellulose ftalato, hydroxylmethylcellulose, 6 quitosano, 7 shellac, 8 carboxylmethylcellulose, 9 acetate of cellulose ftalato, 10 inulina and cyclodextrins, among others, it has been investigated exploring their potential to be used in the liberation of drugs in the colon [11][12][13] and also the design of functional ingredients that should be present in the colon in enough quantity in order to exercise their beneficial effect, as the case of probiotic microorganisms. 14 Pectins belong to a group of substances generally termed dietary fibers, which are the essential part of the plant and have numerous beneficial effects on human health.…”

Section: Introductionmentioning

confidence: 99%

Thermal, Mechanical and Microstructures Properties of Cellulose Derivatives Films: A Comparative Study

et al. 2010

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The proposal in this study was to evaluate the physical properties of different biopolymers films. The materials used were: pectin, carboxyl methylcellulose, methylcellulose, hydroxyl propylcellulose, hydroxypropylmethylcellulose, and corn waxy starch; from these polysaccharides aqueous dispersions were prepared to 3% (w/v) for obtained films. In these biopolymer films, the thermal diffusivities (α) was evaluated by the Open Photoacoustic Cell method; also, their mechanical properties as tensile strength, elongation, and Young's modulus were measured, their crystallinity percentage was evaluated by X-ray diffraction and microstructure through atomic force microscopy in contact mode. From the polysaccharide films, it was observed that most of them were flexible and transparent. In the case of the films, mechanical properties were found that the highest value of tensile strength and Young's modulus corresponded to carboxyl methylcellulose with 69.17 and 1,912.20 MPa values, respectively. Also, Open Photoacoustic Cell method and X-ray diffraction measurements showed that there exist a correlation between the thermal diffusivity values and the crystallinity measured in the biopolymer films. It was also observed that α values of cellulose derived was affected by the substitution group in the molecule, reaching the highest α value, the films of carboxyl methylcellulose. Regarding the microstructural of the films, starch showed the highest roughness value (88.6 nm) whereas hydroxypropyl-methylcellulose resulted with the lowest roughness value (7.67 nm).

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“…The PRIT-5 M. hyopneumoniae strain isolated by the Animal Technology Institute, Taiwan, ROC, was cultured in Friis medium [9] and treated as in our previous investigation [17]. The culture was harvested when the medium's O.D.…”

Section: Preparation Of Microspheresmentioning

confidence: 99%

Protective Effects of Oral Microencapsulated Mycoplasma hyopneumoniae Vaccine Prepared by Co-Spray Drying Method.

Lin

Weng

Liao

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. The efficacy of Mycoplasma hyopneumoniae oral vaccine was investigated in microsphere dosage form. A co-spray drying process was used to apply an encapsulating material, Eudragit L30 D-55, to microspheres containing Mycoplasma hyopneumoniae antigens. The microspheres were generally effective (>93%) with protein release at pH 7.4, but almost none were released at pH 1.2, for 3 hr in an in vitro dissolution test. An SPF-swine model was used to evaluate the effectiveness of the microspheres as an oral vaccine, and the related immune responses. The serum's systemic IgG against M. hyopneumoniae was evoked by ELISA analysis, after a 2nd immunization of all pigs. The vaccinated groups' mean lesion score was significantly lower after the Mycoplasma hyopneumoniae challenge than that of the nonvaccinated/challenged groups (P<0.05). This study strongly suggests that the oral microspheres vaccine prepared by a co-spray drying method can provide effective protection against M. hyopneumoniae infection in pigs. KEY WORDS: co-spray drying, microsphere, Mycoplasma hyopneumoniae, oral vaccine.J. Vet. Med. Sci. 65(1): 69-74, 2003 Mycoplasma hyopneumoniae is widely recognized as a potent pathogen for mycoplasmal pneumonia in swine [2], colonizing the respiratory epithelia and compromising integrity by inducing an inflammatory response [20]. The pathogen also causes significant economic losses by reducing body weight and prolonging periods between feeds [22]. Although some drugs and antibiotics are effective in vitro against M. hyopneumoniae, reducing the disease's clinical signs [12], they have not yet been proven effective in eliminating the pathogen in vivo. Vaccination is an essential strategy in controlling mycoplasmal pneumonia. M. hyopneumoniae infects the ciliated epithelial cells of the respiratory tract [6], and a mucosal immune response may therefore be important in the prevention and control of M. hyopneumoniae-induced pneumonia [23]. The concept of a common mucosal immune system has been supported by several oral immunization reports [8,13]. Numerous experimental systems have proven that oral immunization can induce antibody secretion into the mucus on these surfaces [8,16,18], and is almost completely restricted to the secretory form of IgA. Oral immunization causes this IgA antibody to be secreted from the GALT to distant mucosal tissue, including the salivary, mammary, respiratory, intestine and genital mucosal tissue, protecting these areas from pathogen invasion [5,14].Microspheres have recently received widespread interest as vehicles for the controlled release of drug or bioactive agents; resisting degradation in gastric acid, they can release drugs in the intestines [1]. Oral antigen delivery by pHdependent microspheres can improve local and systemic immune responses [4,16], serving as a potent mucosal immunogen. It is essential to develop a new, simple and effective vaccine process. Microsphere oral vaccines have been previously developed in our laboratory via a novel cospray-drying process [16]. C...

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Preparation of enteric-coated microspheres of Mycoplasma hyopneumoniae vaccine with cellulose acetate phthalate: I. Formation condition and micromeritic properties

Cited by 27 publications

References 14 publications

Tioconazole Loaded Mucoadhesive Microspheres for the Treatment of Vaginal Candidiasis

Tioconazole Loaded Mucoadhesive Microspheres for the Treatment of Vaginal Candidiasis

Thermal, Mechanical and Microstructures Properties of Cellulose Derivatives Films: A Comparative Study

Protective Effects of Oral Microencapsulated Mycoplasma hyopneumoniae Vaccine Prepared by Co-Spray Drying Method.

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