Preparation of DNA-Based Biosensor for Electrochemically Identification of Transgenic Soybean

Abstract

Since the successful transformation of the first transgenic soybean, the variety and planting area of transgenic soybean have increased. Therefore, transgenic soybean identification has become a necessary procedure in gm research. The development of rapid, sensitive, high-throughput and newly automated detection methods has become a hot spot in the detection of transgenic products. In this paper, we modified a layer of a partially reduced graphene oxide film on the surface of an ionic liquid carbon paste electrode by a green, controlled electrochemical reduction method. We fixed the probe on the electrode surface by covalently bonding the remaining oxygen-containing groups on the electrode surface with an end-modified amino group. Then, the hybridization reaction was detected with methylene blue as an indicator after hybridization between the electrode and the target sequence, and the transgenic soybean sequence was detected by differential pulse voltammetry. We also tested PCR products of DNA extracted from actual samples.

Advances in Electrochemical Techniques for the Detection and Analysis of Genetically Modified Organisms: An Analysis Based on Bibliometrics

“…Jamaluddin et al [60] used anthraquinone-2-sulphonic acid as a marker. Deng et al [61] used a label-free sensing technology to directly reflect the differences in DNA hybridization with changes in electrode performance. Ge et al [62] recently reported a CRISPR/Cas12a-mediated dual-mode PCR, genosensor, and sequence became burst keywords over the next few years, but they did not last very long.…”

“…GM algae were used to enhance the sensing capabilities of the platform, which could be used to identify different herbicide subclasses. The authors of the second paper proposed a labeled electrochemical sensor to detect GM soybean [61]. This paper is also classified in Cluster #0.…”

Eletrossíntese orgânica e caracterização do copolímero poli(anilina-co-4-aminofenol) para aplicação em imunossensor de biomarcador de distúrbios da tireoide

“…Como os biossensores geralmente envolvem detecções de analitos em concentrações baixas ou o evento de bioreconhecimento produz um sinal baixo, os transdutores eletroquímicos são muito utilizados por serem sensíveis a pequenas mudanças na eletroatividade do eletrodo (DENG et al, 2020). Outras vantagens de biossensores eletroquímicos incluem: rápidos tempos de resposta; facilidade na aplicação e leitura dos dados; baixo custo e possibilidade de miniaturização (BLAIR e CORRIGAN, 2020).…”