Preparation of cell envelopes of large numbers of individual bacterial strains with the use of an automatic cell disruptor

Alphen, Loek van; Romijn, C.; Brandt, Heike; Geelen, L.; Zanen, H. C.

doi:10.1016/0003-2697(87)90542-2

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Cited by 4 publications

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“…Isolation of cell envelopes and analysis of the OMP composition by SDS-PAGE was done essentially as described by Lugtenberg et al (17). The process of cell disruption was automated as described by Van Alphen et al (30). The OMP patterns of the isolates were compared with those of the OMP subtype reference strains of Hib used in the Van Alphen system (29) and the 23 OMP subtype reference strains used in the Barenkamp system (3).…”

Section: Methodsmentioning

confidence: 99%

Molecular epidemiology of Haemophilus influenzae type b in the Gambia

et al. 1992

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Section: Methodsmentioning

confidence: 99%

Molecular epidemiology of Haemophilus influenzae type b in the Gambia

et al. 1992

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Cross-infection by non-encapsulated Haemophilus influenzae

Gough¹,

Kraak²,

Anderson³

et al. 1990

The Lancet

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Changes in Outer Membrane Proteins of Nontypable Haemophilus influenzae in Patients with Chronic Obstructive Pulmonary Disease

Groeneveld¹,

Alphen²,

Eijk³

et al. 1988

Journal of Infectious Diseases

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Five individual colonies of Haemophilus influenzae were isolated from each of one to three cultures of sputum collected from 18 patients with chronic obstructive pulmonary disease (COPD). The isolates were studied to investigate whether the major outer membrane proteins (MOMPs) changed during persistence. The relationship between isolates was analyzed by fingerprinting their chromosomal DNA. The fingerprints of eight strains (isolated from eight patients) with various MOMP compositions were different, whereas fingerprints of isolates with identical MOMP compositions were indistinguishable. In 12 patients, two or more strains with different MOMP compositions were found; one strain was isolated from the sputum samples of each of the six remaining patients. In seven of the 12 patients, strains with different MOMPs but with indistinguishable fingerprints were found. The differences were found in proteins b,c (five patients) and d (five patients). In patients with COPD, the MOMPs of H. influenzae are subject to changes that may enable this bacterium to escape immunological defense mechanisms.

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Preparation of cell envelopes of large numbers of individual bacterial strains with the use of an automatic cell disruptor

Cited by 4 publications

References 6 publications

Molecular epidemiology of Haemophilus influenzae type b in the Gambia

Molecular epidemiology of Haemophilus influenzae type b in the Gambia

Cross-infection by non-encapsulated Haemophilus influenzae

Changes in Outer Membrane Proteins of Nontypable Haemophilus influenzae in Patients with Chronic Obstructive Pulmonary Disease

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