Molecular epidemiology of Haemophilus influenzae type b in the Gambia

Bijlmer, H. A.; Alphen, L. Van; Broek, L Geelen-van den; Greenwood, Brian; Valkenburg, H. A.; Dankert, J.

doi:10.1128/jcm.30.2.386-390.1992

Cited by 8 publications

(1 citation statement)

References 28 publications

(30 reference statements)

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“…This technique may therefore be useful in clarifying the strain(s) endemic in certain regions. Epidemiological studies of Hib infections commonly use phenotyping (serotyping, biotyping, and analysis of the major outer membrane protein patterns) (3,15) or ribotyping methods (14,18,19). Recently, epidemiological studies of Hib strains isolated from vaccinated patients have been performed.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiological Study ofHaemophilus influenzaeSerotype b Strains Obtained from Children with Meningitis in Japan

et al. 1999

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We report an epidemiological study of 30 Haemophilus influenzae serotype b (Hib) strains derived from the cerebrospinal fluid of children with meningitis. The Hib strains were biotyped, tested for β-lactamase production, and genotyped by long PCR-ribotyping, random amplified polymorphic DNA (RAPD) analysis, and genomic DNA restriction fragment length polymorphism (RFLP) analysis by pulsed-field gel electrophoresis (PFGE). The phenotypic study characterized 22 of the strains (73%) as biotype I. A genotypic study using long PCR-ribotyping withHaeIII restriction digestion showed no polymorphisms among these 30 Hib strains, but RAPD analysis with two sets of primers demonstrated two distinctive subtypes: one typical of the strains of biotype group II and the second characteristic of the strains of biotype groups I and IV. Each RAPD group was subtyped into several genotypic groups by PFGE-RFLP with SmaI digestion. The genotyping of clinically isolated Hib strains may help to elucidate transmission routes in community infections, endemicity, and the reasons for vaccine failure.

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Section: Discussionmentioning

confidence: 99%

Molecular Epidemiological Study ofHaemophilus influenzaeSerotype b Strains Obtained from Children with Meningitis in Japan

et al. 1999

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Molecular Typing in Bacterial Infections Haemophilus spp.

Cerquetti

2012

Molecular Typing in Bacterial Infections

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Haemophilus influenzae: Then and now

Jordens

Slack

1995

Eur. J. Clin. Microbiol. Infect. Dis.

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Haemophilus influenzae has long been recognised as a major cause of serious infection and mortality in children less than 5 years old. Prior to the introduction of Haemophilus influenzae type b (Hib) immunisation, the incidence of a child suffering an invasive Haemophilus infection was 20-50/100,000 in industrialised countries and up to ten times higher in developing regions. The introduction of a Hib vaccine programme results in a rapid and dramatic decline in the incidence of Hib infection in the susceptible childhood population. For example, within two years of the introduction of routine Hib vaccination of infants in the UK, the risk of serious Hib infection had fallen from 1:600 to 1:30,000 by 5 years of age. Many other European countries have introduced, or are in the process of introducing, a routine Hib immunisation programme. Because the epidemiology of Haemophilus influenzae infection is changing so dramatically, it is opportune to review Haemophilus influenzae as it was perceived in the pre-vaccine era (the past) and during vaccine implementation (the present), and how its role may change in the post-vaccination era (the future). This review will summarise the historical landmarks that have led to our present-day understanding of Haemophilus influenzae pathogenicity, the concerns about antibiotic resistance, the features of the host immune response to Haemophilus influenzae, and the introduction of the Hib vaccine. Furthermore, the possible importance of this organism in the future will be discussed.

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Molecular epidemiology of Haemophilus influenzae type b in the Gambia

Cited by 8 publications

References 28 publications

Molecular Epidemiological Study ofHaemophilus influenzaeSerotype b Strains Obtained from Children with Meningitis in Japan

Molecular Epidemiological Study ofHaemophilus influenzaeSerotype b Strains Obtained from Children with Meningitis in Japan

Molecular Typing in Bacterial Infections Haemophilus spp.

Haemophilus influenzae: Then and now

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