Molecular Epidemiological Study of<i>Haemophilus influenzae</i>Serotype b Strains Obtained from Children with Meningitis in Japan

Mitsuda, Toshihiro; Kuroki, Haruo; Ishikawa, Naoki; Imagawa, Tomoyuki; Ito, Schuichi; Miyamae, Takako; Mori, Masaaki; Uehara, Suzuko; Yokota, Shumpei

doi:10.1128/jcm.37.8.2548-2552.1999

Cited by 15 publications

(2 citation statements)

References 19 publications

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“…Three of these patterns were composed of a single band of 884, 1,265, 650 bp and were designated RAPD patterns A, B, and C, respectively. One was composed of a clear band of 420 bp and three weak bands of 1,265, 918, and 444 bp, and it was designated RAPD pattern D. The RAPD pattern A was produced with DNAs extracted from the type strain of E. rhusiopathiae and from the reference strains of serovars 1a, 1b, 2, 4, 5,6,8,9,11,12,15,16,17,19, and 21 and type N, believed to be E. rhusiopathiae. The RAPD pattern B was produced with DNAs extracted from the type strain of E. tonsillarum and from the reference strains of serovars 3, 7, 10, 14, 20, 22, and 23, considered to be E. tonsillarum.…”

Section: Resultsmentioning

confidence: 99%

Randomly Amplified Polymorphic DNA Analysis of Erysipelothrix spp

et al. 2000

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The usefulness of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was studied. Eighty-one strains and 18 random primers were tested. Among the tested primers, the primers NK51 (GGTGGTGGTATC) and NK6 (CCCGCGCCCC) produced noticeable results. The primer NK51 revealed four species-specific RAPD patterns. Of the 66 strains of E. rhusiopathiae, 64 had the same unique band of 884 bp. Of the 12 strains of E. tonsillarum, 11 produced a 1,265-bp band. In addition, two strains, previously thought to be E. rhusiopathiae, produced the 1,265-bp band, suggesting that they had been misclassified. One strain of E. tonsillarumproduced the 884-bp band, suggesting that it too was E. rhusiopathiae. The E. rhusiopathiae strain of serovar 13 produced a 650-bp band, and the strain of serovar 18 produced a clear 420-bp band as well as three weak bands of 1,265, 918, and 444 bp. The primer NK6 revealed 14 RAPD patterns that were not serovar specific. However, different patterns were produced among strains of the same serovar showing that the RAPD method is able to identify the genetic variations of strains of this genus and can rapidly and easily differentiate strains of the same serovar. Based on these results, we concluded that the RAPD method with primers NK51 and NK6 is a rapid and reliable method to identify the species of this genus; we also concluded that this method might be a useful tool for the epidemiological analysis of the Erysipelothrix species.

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Section: Resultsmentioning

confidence: 99%

Randomly Amplified Polymorphic DNA Analysis of Erysipelothrix spp

et al. 2000

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“…A Japanese study concerning the capsular types of Hi isolates from pediatric patients with respiratory tract infections showed that all encapsulated strains (types b and f) displayed biotype I [8] . Of the 30 Hib isolates from the cerebrospinal fluid of Japanese children with meningitis, 22 (73.3%) were characterized as biotype I [9] . Thus, this biochemical phenotypic assay is useful to assess the prevalent type of Hi based on the data combined with the capsular genotypes.…”

Section: Discussionmentioning

confidence: 99%