Preparation of carbon paste electrodes including poly(styrene) attached glycine–Pt(IV) for amperometric detection of glucose

Dönmez, Soner; Arslan, Fatma; Sarı, Nurşen; Yetim, Nurdan Kurnaz; Arslan, Halit

doi:10.1016/j.bios.2013.10.059

Cited by 27 publications

(16 citation statements)

References 25 publications

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“…7. 5,63,73 Additionally, the current response of the biosensor has unchanged aer 50 laps continuous cyclic voltammetry in 0.1 M PBS (pH 6.5) and 0.5 mM glucose solution, showing its high reproducibility. Furthermore, due to their high specic surface area, OMC composite materials have a great adsorption capacity for active materials and (Fig.…”

Section: Gox + Nife X /Omc + Naon + Gce Detection Of Glucosementioning

confidence: 95%

Amperometric hydrogen peroxide and glucose biosensor based on NiFe₂/ordered mesoporous carbon nanocomposites

Xiang

Yin

et al. 2015

Analyst

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Nanocomposites of NiFex embedded in ordered mesoporous carbon (OMC) (x = 0, 1, 2) were prepared by a wet impregnation and hydrogen reduction process and were used to construct electrochemical biosensors for the amperometric detection of hydrogen peroxide (H2O2) or glucose. The NiFe2/OMC nanocomposites were demonstrated to have a large surface area, suitable mesoporous channels, many edge-plane-like defective sites, and a good distribution of alloyed nanoparticles. The NiFe2/OMC and Nafion modified glass carbon electrode (GCE) exhibited excellent electrocatalytic activities toward the reduction of H2O2 as well. By utilizing it as a bioplatform, GOx (glucose oxidase) cross-linked with Nafion was immobilized on the surface of the electrode for the construction of an amperometric glucose biosensor. Our results indicated that the amperometric hydrogen peroxide biosensor (NiFe2/OMC + Nafion + GCE) showed good analytical performances in term of a high sensitivity of 4.29 μA mM(-1) cm(-2), wide linearity from 6.2 to 42,710 μM and a low detection limit of 0.24 μM at a signal-to-noise ratio of 3 (S/N = 3). This biosensor exhibited excellent selectivity, high stability and negligible interference for the detection of H2O2. In addition, the immobilized enzyme on NiFe2/OMC + Nafion + GCE, retaining its bioactivity, exhibited a reversible two-proton and two-electron transfer reaction, a fast heterogeneous electron transfer rate and an effective Michaelis-Menten constant (K) (3.18 mM). The GOx + NiFe2/OMC + Nafion + GCE could be used to detect glucose based on the oxidation of glucose catalyzed by GOx and exhibited a wide detection range of 48.6-12,500 μM with a high sensitivity of 6.9 μA mM(-1) cm(-2) and a low detection limit of 2.7 μM (S/N = 3). The enzymic biosensor maintained a high selectivity and stability features, and shows great promise for application in the detection of glucose.

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Section: Gox + Nife X /Omc + Naon + Gce Detection Of Glucosementioning

confidence: 95%

Amperometric hydrogen peroxide and glucose biosensor based on NiFe₂/ordered mesoporous carbon nanocomposites

Xiang

Yin

et al. 2015

Analyst

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“…The unmodified CPE was prepared in the same way without adding FMPS‐Gly. The electrode was polished on weighing paper to obtain producible working surface .…”

Section: Methodsmentioning

confidence: 99%

Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere‐attached l‐glycine

Dönmez

Arslan

Sarı

et al. 2017

Biotech and App Biochem

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In the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified with (4-formyl-3-methoxyphenoxymethyl)polystyrene (FMPS) with l-glycine. Polymeric microspheres having Schiff bases were prepared from FMPS using the glycine condensation method. Glucose oxidase enzyme was immobilized onto modified carbon paste electrode by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H O (+0.5 V vs. Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50 °C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0 × 10 to 1.0 × 10 M, R = 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3% relative standard deviation. It also had good storage stability.

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“…After Norman and et al [8] pioneered the development of nanomaterial-based polystyrene in 1993, today many researchers have developed immobilization properties of polystyrene nanospheres [9][10][11]. One of the immobilized species is covalent immobilization.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of acetylcholinesterase on Pt(ii) and Pt(iv) attached nanoparticles for the determination of pesticides

et al. 2015

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Pt(ii) and Pt(iv)-tagged nanoparticles have been synthesized according to the template method for the identification of pesticides. Their morphologies have been investigated using scanning electron microscopy and characterized by means of spectral measurements. Then, acetylcholinesterase (AChE) was immobilized onto the nanoparticles. The AChE immobilized Pt(ii) and Pt(iv)-tagged nanomaterials show high reusability and storage capacity. The catalytic activity of AChE followed Michaelis-Menten kinetics. Assays for enzyme activity measurements demonstrate that the nanospheres tagged with Pt(ii) have a much better performance than those with Pt(iv). Furthermore, whether or not there was any interaction between the immobilized enzyme and 1-naphthyl-N-methylcarbamate, which is a carbamate insecticide, was examined.

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Preparation of carbon paste electrodes including poly(styrene) attached glycine–Pt(IV) for amperometric detection of glucose

Cited by 27 publications

References 25 publications

Amperometric hydrogen peroxide and glucose biosensor based on NiFe₂/ordered mesoporous carbon nanocomposites

Amperometric hydrogen peroxide and glucose biosensor based on NiFe₂/ordered mesoporous carbon nanocomposites

Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere‐attached l‐glycine

Immobilization of acetylcholinesterase on Pt(ii) and Pt(iv) attached nanoparticles for the determination of pesticides

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Preparation of carbon paste electrodes including poly(styrene) attached glycine–Pt(IV) for amperometric detection of glucose

Cited by 27 publications

References 25 publications

Amperometric hydrogen peroxide and glucose biosensor based on NiFe2/ordered mesoporous carbon nanocomposites

Amperometric hydrogen peroxide and glucose biosensor based on NiFe2/ordered mesoporous carbon nanocomposites

Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere‐attached l‐glycine

Immobilization of acetylcholinesterase on Pt(ii) and Pt(iv) attached nanoparticles for the determination of pesticides

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Amperometric hydrogen peroxide and glucose biosensor based on NiFe₂/ordered mesoporous carbon nanocomposites

Amperometric hydrogen peroxide and glucose biosensor based on NiFe₂/ordered mesoporous carbon nanocomposites