Preparation of carbon dot‐based ratiometric fluorescent probes for cellular imaging from Curcuma longa

Abstract

This work derived biocompatible and stable probes based on fluorescent nanoparticles (FNPs) from a natural source, Curcuma longa. The multi-color fluorescence emissions from carbonized Curcuma longa (C-FNPs) obtained through defined dehydration conditions are soluble in water and have a small particle size (~17 nm). The surface passivation with polyethylene glycol (PEG) capped with amine groups in FNPs (P-FNPs) generated a probe with a higher quantum yield and longer fluorescence lifetime than obtained with C-FNPs. The X-ray photoelectron spectroscopy and X-ray diffraction spectra confirmed the associated chemical moieties of C-FNPs and P-FNPs. Furthermore, the prepared material showed non-toxic effects with almost 100% cell viability, even at high concentrations. In conclusion, fluorescence sensors from natural sources may be useful for numerous biomedical research applications.

Dual doped biocompatible multicolor luminescent carbon dots for bio labeling, UV‐active marker and fluorescent polymer composite

“…Magnesium doping to tamarind extract associated with nitrogen-doping is for the first time reported here which also produce very high quantum yield. [9,10] The unique features of C-dots make them potential applicants in the field of bio-labeling, [11][12][13][14][15] drug delivery, [16] luminescent membranes, [17] fluorescent sensors, [18][19][20][21][22][23][24] light emitting diodes (LEDs), [25] catalytic activity, [26] storage applications, [27] and renewable energy harvesting. More importantly, the chemical signatures of the carbon dots unveiled in this work can support their easy solubilization into water; even in sub-ambient temperature.…”

“…Carbon nanomaterials, including fullerenes, [1] carbon nanotubes, [2,3] graphene [4] have gained remarkable attention owing to their unique properties and potential applications, including electrode materials, catalysis, adsorption, and gas storage among others. [9,10] The unique features of C-dots make them potential applicants in the field of bio-labeling, [11][12][13][14][15] drug delivery, [16] luminescent membranes, [17] fluorescent sensors, [18][19][20][21][22][23][24] light emitting diodes (LEDs), [25] catalytic activity, [26] storage applications, [27] and renewable energy harvesting. [5][6][7][8] Typically displaying size and excitation dependent luminescence properties, Cdots have received great attention in the present scenario as nascent nano-dots, especially in the field where size, cost of the material and biocompatibility are critical.…”

Dual‐Responsive Carbon Dot for pH/Redox‐Triggered Fluorescence Imaging with Controllable Photothermal Ablation Therapy of Cancer

“…The complete breakdown of the crosslinked sites in response to GSH and acid treatment (pH 6.8) resulted in small‐sized particles from FNP nanoparticles, as shown in Figure b. The small black dots in TEM images indicate lattice planes of graphitic (sp 2 ) with planar distances of 0.33–0.34 nm, thereby confirming the successful application of the carbonization technique on the polymer . Figure c shows that the initial decay time of FNP ( τ 0 =8.68589 ns) decreased ( τ 1 =1.34631 ns) after IR825 loading.…”

“…The small black dots in TEM images indicate lattice planes of graphitic (sp 2 )w ith planar distances of 0.33-0.34 nm, thereby confirmingt he successful application of the carbonization technique on the polymer. [23,24] Figure 2c shows that the initial decay time of FNP (t 0 = 8.68589 ns) decreased (t 1 = 1.34631 ns) after IR825 loading. On the other hand, the treatment of the samples with GSH (10 mm)and acidic (pH 6.8) solutionresulted in the recovery of the decay time to t 2 = 8.24862 ns.…”

Precursor Concentration Effect on Optical Properties of Carbon Dots from Cassava’s Peels

“…The absorbance peaks of C-dots is strongly influenced by the concentration of precursors during synthesis. Absorption spectrum at concentrations of 0.25%, 0.5%, 1.0% and 2.0% respectively contained at wavelength peaks of 304.67 nm, 307.97 nm, 327.72 nm and 344.15 nm which showed a transition state n-π * in the structure of aromatic bonds C = O originating from the surface of C-dots (Asha Jhonsi & Kathiravan, 2017; Han et al, 2017;Li et al, 2018;Mazrad, Kang, In, & Park, 2018). In this case, the more carbon dots are formed with increasing precursor concentrations.…”