Preparation of boronate affinity monolithic column with high specific surface using highly structurally symmetrical copolymerizing reagents

Abstract

Boronate affinity monolithic column is an important chromatographic medium for the selective isolation of the cis-diol containing compounds. However, it is difficult to control the micro-structure of the monolithic capillary during its preparation process. In this article, using melamine and tris (2,3-epoxypropyl) isocyanurate (TEPIC), both are highly symmetrical in molecular structure, as the copolymerizing reagents, a boronate affinity monolithic capillary with high specific surface was prepared by in-situ polymerization according to the "teamed boronate affinity" principle. The pH for binding with cis-diol containing compounds of this monolithic capillary was decreased to neutral range due to the teamed bonorate affinity. The specific surface area was dramatically increased due to the use of the highly symmetrical copolymerizing reagents, which was 80.3 m2/g and apparently higher than those for other boronate affinity monolithic capillaries reported in references.

A high boronate avidity monolithic capillary for the selective enrichment of trace glycoproteins

“…This is an apparent limitation, since it leads to not only inconvenience of pH adjustment but also the risk of degradation of labile compounds. In recently years, our group has dedicated to reduce the binding pH of boronic acid-functionalized materials through using boronic acid with special structure [21,29,38,41,45,54] or the teamed boronate affinity strategy [20,28,37,56]. Usually the lowest binding pH is provided to indicate the lowest pH condition of the sample environment a boronic acid-functionalized material can be applicable.…”