Preparation of Biocompatible Materials and Their Evaluation

Nakabayashi, Nobuo

doi:10.1002/masy.200651385

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…In any case, the strong antifouling properties of MPC-based materials are well known and have the potential to improve the biocompatibility of new materials. 18 There are, however, limitations associated with the use of antifouling coatings and films. 19 Long-term stability to oxidative degradation and chain cleavage, reliance on defect-free coatings and susceptibility to penetration of low molecular weight proteins and peptides are all issues that need to be addressed.…”

Section: Introductionmentioning

confidence: 99%

Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties

et al. 2013

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The antibacterial (bioactive) and antifouling (biopassive) properties of stable, uniform, high surface coverage films of poly(hydroxyethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) ( p(HEMAco-MPC)) with embedded, non-leaching silver nanoparticles (AgNPs) are reported. Based on the experimental findings, a mechanism of action of AgNPs in antibacterial activity in combination with antifouling characteristics is discussed. Long-term antifouling studies of E. coli determine little to no adhesion on p(HEMA-co-MPC)/Ag films at 2.5 × 10 6 CFU mL −1 for 7 d, measured using live/dead staining assays. Agar diffusion tests indicate that there is no leaching of Ag from the films and SEM and EDX analyses of the films before and after incubation with E. coli show no attachment of E. coli and no visible change in film morphology or AgNP dispersal. Antibacterial studies are investigated using E. coli K-12 as a model bacterial strain and are tested in static (CFUs) and dynamic contact assays. Antibacterial efficacy of the films containing extremely low AgNP concentration (3.8 ng cm −2 ) is shown with growth suppression of E. coli in culture medium for 4 h at 1.35 × 10 5 CFU mL −1 and killing greater than 99% of E. coli in only 1 h of exposure to concentrations up to 1 × 10 5 CFU mL −1 . These hybrid films may propose an exciting direction to long-term antibacterial and antifouling films in clinical applications. † Electronic supplementary information (ESI) available: Additional SEM and cLSM images of the films. See

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Section: Introductionmentioning

confidence: 99%

Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties

et al. 2013

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“…One of the most common monomers utilized in biological and biomedical applications is 2‐methacryloyloxyethylphosphorylcholine (MPC), which has biomimetic PC groups in its structure and can polymerize with other hydrophobic monomers 15, 16. It has been extensively shown previously that surface modifications with MPC polymers are effective in improving hemocompatibility by suppressing protein adsorption, platelet adhesion, and platelet activation on blood‐contacting materials such as dialysis membranes, vascular prosthesis, stents, and VADs 21–31. In the report on VAD surface modification, MPC was adsorbed as opposed to covalently attached, leading to erosion of the coating during chronic use 27…”

Section: Introductionmentioning

confidence: 99%

“…15,16 It has been extensively shown previously that surface modifications with MPC polymers are effective in improving hemocompatibility by suppressing protein adsorption, platelet adhesion, and platelet activation on blood-contacting materials such as dialysis membranes, vascular prosthesis, stents, and VADs. [21][22][23][24][25][26][27][28][29][30][31] In the report on VAD surface modification, MPC was adsorbed as opposed to covalently attached, leading to erosion of the coating during chronic use. 27 The objective of this study was to develop a surface modification strategy that could reduce platelet deposition onto the TiAl 6 V 4 surface that we are currently utilizing in the construction of a pediatric VAD where blood biocompatibility concerns are of particular importance.…”

Section: Introductionmentioning

confidence: 99%

Covalent surface modification of a titanium alloy with a phosphorylcholine‐containing copolymer for reduced thrombogenicity in cardiovascular devices

Johnson

Woolley

et al. 2008

J Biomedical Materials Res

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Our objective was to develop a surface modification strategy for a titanium alloy (TiAl6V4) to provide thromboresistance for surfaces in rigorous blood-contacting cardiovascular applications, such as that found in ventricular assist devices. We hypothesized that this could be accomplished by the covalent attachment of a phospholipid polymer, poly(2-methacryloyloxyethylphosphorylcholine (MPC)-co-methacryl acid) (PMA). TiAl6V4 was H2O plasma treated by radio frequency glow discharge, silanated with 3-aminopropyltriethoxysilane (APS), and ammonia plasma treated to increase surface reactivity. The TiAl6V4 surface was then modified with PMA via a condensation reaction between the amino groups on the TiAl6V4 surface and the carboxyl groups on PMA. The surface composition was verified by X-ray photoelectron spectroscopy, confirming successful modification of the TiAl6V4 surfaces with APS and PMA as evidenced by increased Si and P. Plasma treatments with H2O and ammonia were effective at further increasing the surface reactivity of TiAl6V4 as evidenced by increased surface PMA. The adsorption of ovine fibrinogen onto PMA-modified surfaces was reduced relative to unmodified surfaces, and in vitro ovine blood contact through a rocking test revealed marked reductions in platelet deposition and bulk phase platelet activation relative to unmodified TiAl6V4 and polystyrene controls. The results indicate that the PMA-modification scheme for TiAl6V4 surfaces offers a potential pathway to improve the thromboresistance of the blood-contacting surfaces of cardiovascular devices.

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Preparation of Biocompatible Materials and Their Evaluation

Cited by 2 publications

References 21 publications

Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties

Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties

Covalent surface modification of a titanium alloy with a phosphorylcholine‐containing copolymer for reduced thrombogenicity in cardiovascular devices

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