Preparation of autologous bone marrow grafts for cryopreservation using the AS104 cell separator

We assessed autologous bone marrow (BM) harvest and hematologic recovery after high-dose chemotherapy (HDCT) in patients who failed to achieve peripheral blood stem cell (PBSC) mobilization. One hundred and ninety-three patients with germ cell tumor, malignant lymphoma, sarcoma or medulloblastoma were scheduled for HDCT. In 123 patients, PBSC were mobilized by disease-specific chemotherapy plus granulocyte colony-stimulating factor (G-CSF). In 110/ 123 patients (89%) with circulating CD34+ cell counts 2 > or = 10/microl, sufficient hematopoietic autografts were collected (group A). In 13/123 patients (11%) with peripheral CD34 + cell counts < 10/microl, PBSC harvesting was not performed (group B). These latter patients were classified as "poor mobilizers" and underwent second-line BM harvest at a median of 46 (range 10-99) days after mobilization failure. Seventy patients with first-line BM harvest (group C) acted as historical controls. Ten patients from group B proceeded to HDCT and nine were evaluable for hematopoietic reconstitution. Recovery to neutrophils >0.5 x 10(9)/l was comparable with group C patients: 16 (range 9-34) days vs 13 (range 8-98) days. However, platelet (PLT) reconstitution >20 x 10(9)/l was significantly slower, with a median of 35 (range 13-50) days as compared with 19 (range 9-148) days (P = 0.0106) for control patients. Supportive care requirements, febrile days and length of hospital stay were not significantly different between the two groups of patients. We conclude that patients who fail to mobilize PBSC should be evaluated for second-line BM harvest. This approach may preserve the therapeutic option of HDCT for these patients.

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Successful autologous bone marrow rescue in patients who failed peripheral blood stem cell mobilization

Rick¹,

Beyer

Kingreen³

et al. 2000

Annals of Hematology

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Impact of harvest product volume in erythrocyte depletion of allogeneic or autologous bone marrow using COBE spectra

Soydan

Ayyildiz

Dalva

et al. 2007

Transfusion and Apheresis Science

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Bone Marrow Processing Using the Fenwal CS-3000 Plus Blood Cell Separator: Results of 99 Procedures

González‐Campos¹,

Carmona-González²,

Rodriguez-Fernandez³

et al. 2000

Journal of Hematotherapy & Stem Cell Research

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BMT is used as an established therapy for patients with malignant and nonmalignant diseases. Many techniques for ex vivo treatment have been developed, but these techniques must be preceded by BM processing. We report our experience in processing 99 BM using the Fenwal CS-3000 Plus cell separator using the 1-special program. Ninety-nine procedures were performed in BM harvested from 73 patients and 26 healthy donors. The number of nucleated cells (NC), mononuclear cells (MNC), RBC, platelets, colony-forming units-granulocyte-macrophage (CFU-GM), CD34+ cells, relative purity of MNC and PMN, and volume were determined in the unprocessed BM and in the final product. BM processing resulted in NC, MNC, CFU-GM, and CD34+ cell recoveries of 31%, 82.2%, 117.6%, and 97.8%, respectively. RBC, PMN, platelets, and volume removal, respectively, were 96%, 92%, 37.2%, and 85.1%. In pediatric patients, the volume reduction was significantly lower than in adult patients (79.6% versus 88.8%). No other significant differences were found between pediatric and adult results. We conclude that BM processing with the Fenwal CS-3000 Plus cell separator provides a product that can undergo further ex vivo treatments or cryopreservation.

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Preparation of autologous bone marrow grafts for cryopreservation using the AS104 cell separator

Cited by 3 publications

References 16 publications

Successful autologous bone marrow rescue in patients who failed peripheral blood stem cell mobilization

Successful autologous bone marrow rescue in patients who failed peripheral blood stem cell mobilization

Impact of harvest product volume in erythrocyte depletion of allogeneic or autologous bone marrow using COBE spectra

Bone Marrow Processing Using the Fenwal CS-3000 Plus Blood Cell Separator: Results of 99 Procedures

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