Preparation of antibacterial chlorhexidine/vermiculite and release study

Samlíková, Magda; Holešová, Sylva; Hundáková, Marianna; Pazdziora, Erich; Jankovič, Ľuboš; Valášková, Marta

doi:10.1016/j.minpro.2016.12.002

Cited by 17 publications

(11 citation statements)

References 27 publications

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“…After treatment of ZnO/V with CH by mechanical stirring (Figure 2a and Figure 3a), new reflections appeared for sample ZnO/V_M_CH. The interlayer distance values d = 2.96 nm, 2.158 nm, 1.649 nm, and 1.069 nm correspond to these reflections, which confirms the expansion of interlayer space of vermiculite due to intercalation of CH [12,26]. Moreover, the XRD pattern confirms the reflections of non-intercalated CH on the surface of the V substrate.…”

Section: Resultssupporting

confidence: 56%

Effects of Ultrasound on Zinc Oxide/Vermiculite/Chlorhexidine Nanocomposite Preparation and Their Antibacterial Activity

et al. 2019

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Microbial infection and biofilm formation are both problems associated with medical implants and devices. In recent years, hybrid organic-inorganic nanocomposites based on clay minerals have attracted significant attention due to their application potential in the field of antimicrobial materials. Organic drug/metal oxide hybrids exhibit improved antimicrobial activity, and intercalating the above materials into the interlayer of clay endows a long-term and controlled-release behavior. Since antimicrobial activity is strongly related to the structure of the material, ultrasonic treatment appears to be a suitable method for the synthesis of these materials as it can well control particle size distribution and morphology. This study aims to prepare novel, structurally stable, and highly antimicrobial nanocomposites based on zinc oxide/vermiculite/chlorhexidine. The influence of ultrasonic treatment at different time intervals and under different intercalation conditions (ultrasonic action in a breaker or in a Roset’s vessel) on the structure, morphology, and particle size of prepared hybrid nanocomposite materials was evaluated by the following methods: scanning electron microscopy, X-ray diffraction, energy dispersive X-ray fluorescence spectroscopy, carbon phase analysis, Fourier transforms infrared spectroscopy, specific surface area measurement, particle size analysis, and Zeta potential analysis. Particle size analyses confirmed that the ultrasonic method contributes to the reduction of particle size, and to their homogenization/arrangement. Further, X-ray diffraction analysis confirmed that ultrasound intercalation in a beaker helps to more efficiently intercalate chlorhexidine dihydrochloride (CH) into the vermiculite interlayer space, while a Roset’s vessel contributed to the attachment of the CH molecules to the vermiculite surface. The antibacterial activity of hybrid nanocomposite materials was investigated on Gram negative (Escherichia coli, Pseudomonas aeruginosa) and Gram positive (Staphylococcus aureus, Enterococcus faecalis) bacterial strains by finding the minimum inhibitory concentration. All hybrid nanocomposite materials prepared by ultrasound methods showed high antimicrobial activity after 30 min, with a long-lasting effect and without being affected by the concentration of the antibacterial components zinc oxide (ZnO) and CH. The benefits of the samples prepared by ultrasonic methods are the rapid onset of an antimicrobial effect and its long-term duration.

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Section: Resultssupporting

confidence: 56%

Effects of Ultrasound on Zinc Oxide/Vermiculite/Chlorhexidine Nanocomposite Preparation and Their Antibacterial Activity

et al. 2019

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“…After NaVER intercalation with CA (Figure 1b), the interlayer space expanded to d = 2.933 nm, d = 2.140 nm, d = 1.586 nm, and d = 1.067 nm. These new series of reflections confirmed intercalation of CA into the VER interlayer [11].…”

Section: Resultsmentioning

confidence: 77%

“…Interlayer distances shifted to d = 2.981 nm, d = 2.210 nm, and d = 1.134 nm. These changes may signify the release of a small amount of CA from the VER interlayer and reorganization of CA molecules in the VER interlayer [11,12,13].…”

Section: Resultsmentioning

confidence: 99%

Study of the Structure and Antimicrobial Activity of Ca-Deficient Ceramics on Chlorhexidine Nanoclay Substrate

et al. 2019

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Novel biomedical composites, based on organically modified vermiculite and montmorillonite with deposited Ca-deficient hydroxyapatite (CDH), were prepared. The monoionic sodium forms of vermiculite and montmorillonite were intercalated with chlorhexidine diacetate (CA). The surfaces of organoclays were used for the precipitation of Ca-deficient hydroxyapatite. The composites with Ca-deficient hydroxyapatite showed very good antibacterial effects, similar to the antimicrobial activity of pure organoclay samples. Better antibacterial activity was shown in the organically modified montmorillonite sample with Ca-deficient hydroxyapatite compared with the vermiculite composite, but, in the case of Staphylococcus aureus, both composites showed the same minimum inhibitory concentration (MIC) value. The antimicrobial effect of composites against bacteria and fungi increased with the time of exposure. The structural characterization of all the prepared materials, performed using X-ray diffraction and FT infrared spectroscopy analysis, detected no changes in the original clay or CDH during the intercalation or precipitation process, therefore we expect the strength of the compounds to be in the original power.

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“…Their natural abundance and low toxicity has contributed to their widespread application and has made clay minerals popular materials for drug delivery research. An increasing body of research (Rodrigues et al, 2013;Viseras et al, 2010) is being published on the subject of using clay minerals to deliver a range of antibacterial molecules such as chlorhexidine (Samlíková et al, 2017), ciprofloxacin (Hamilton et al, 2014;Rivera et al, 2016), clindamycin (Porubcan et al, 1978), gentamicin (Rapacz-kmita et al, 2017), vancomycin (Pan et al, 2017), and ofloxacin (Wang et al, 2014), amongst others. The interaction between clay-minerals and drug-molecules is also reported to yield a controlled-release of the adsorbed drug molecules (Park et al, 2008;Rivera et al, 2016), which is attractive for many pharmaceutical applications including patients.…”

Section: Introductionmentioning

confidence: 99%

Formulation and antibacterial properties of clay mineral-tetracycline and -doxycycline composites

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Roberts

Hutcheon

et al. 2019

Applied Clay Science

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Preparation of antibacterial chlorhexidine/vermiculite and release study

Cited by 17 publications

References 27 publications

Effects of Ultrasound on Zinc Oxide/Vermiculite/Chlorhexidine Nanocomposite Preparation and Their Antibacterial Activity

Effects of Ultrasound on Zinc Oxide/Vermiculite/Chlorhexidine Nanocomposite Preparation and Their Antibacterial Activity

Study of the Structure and Antimicrobial Activity of Ca-Deficient Ceramics on Chlorhexidine Nanoclay Substrate

Formulation and antibacterial properties of clay mineral-tetracycline and -doxycycline composites

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