Copper based metal-organic frameworks (Cu-BTC MOFs)/ polyacrylonitrile (PAN) composite films were fabricated by embedding the MOF crystals in soluble polyacrylonitrile (PAN) at different Cu-BTC MOF loadings of 5-40 mass %. The obtained film is highly porous and acts as a house for hosting the bioactive MOF. These Cu-BTC/PAN composite films were then evaluated for antimicrobial application. Data presented herein show that the PAN does not notably interfere with the different strain of bacteria,Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa where the the antimicrobial activity is linearly increase with the increase in Cu-BTC MOF loadings. The results indicated that the best time for elemination of Bacillus subtilize, E. coli , and Candida albicans was 4, 2, and 6 hours respectively.While elimination of Staphylococcus aureus in time (2, 4,and 6 hours) has the same impact. Cu-BTC sheet had the option to eliminate Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa in both beginning inoculums of 1% and 10% with improved impact on account of 1 %inoculum. Cu-BTC was able to eliminate microorganisms when staying in a mixed co-culture which reduced the cell from 100,000 to 1 CFU/ml.