Preparation of an ion-selective electrode sensitive to flufenamic acid anion and its application to the study of protein binding of flufenamate in aqueous solution.

Kiryu, Setsuo; Oda, Y.; Sasaki, Masanori

doi:10.1248/cpb.31.1089

Cited by 9 publications

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“…Similar methods were used to determine the parameters for the binding of bile acids to bovine serum albumin using an ISE for bile acids (14). An ISE for flufenamic acid has also been reported and its response studied in the presence of bovine albumin, but no binding parameters were determined (15). The ISE for dodecyl sulfate and octyl or decyl sulfate were used to study the binding of these ions to bovine albumin (16) and /3-lactoglobulin (17), respectively.…”

Section: Introductionmentioning

confidence: 99%

Binding studies using ion-selective electrodes. Examination of the picrate-albumin interaction as a model system

Christopoulos¹,

Diamandis²

1990

Anal. Chem.

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We are studying the binding of ligands to macromolecules by using ligand ion selective electrodes as transducers. The picrate-bovine albumin interaction is examined in detail as a model system. A picrate ion selective electrode is used to monitor the free picrate concentration directly in the presence of albumin and bound ligand. The binding parameters are estimated and the effect of protein concentration, ionic strength, pH, and temperature is studied. The experimental data are interpreted with a specially designed computer program that performs nonlinear least-squares fitting of the generalized Scatchard model with an infinite number of classes of binding sites directly to the raw potentiometric data. The binding parameters (binding constant and maximum number of ligands that can be bound), the nonspecific binding as well as their standard deviations are estimated by this program. The principles described can be used for the potentiometric study of any ligand-binder interaction.

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Section: Introductionmentioning

confidence: 99%

Binding studies using ion-selective electrodes. Examination of the picrate-albumin interaction as a model system

Christopoulos¹,

Diamandis²

1990

Anal. Chem.

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Section: Introductionmentioning

confidence: 99%

“…1, 2008 To the best of our knowledge, there are a single report on the use of ion-selective potentiometric sensor for the determination of flufenamic acid. 25 However, this electrode has not been applied for the determination of FFA in pharmaceuticals and biological fluids, thus, precluding the assessment of its usefulness in real analysis.Previous work from this laboratory dealt with the development of mercury(I)-carboxylate electrodes and their application to solution equilibria, [26][27][28][29] food analysis, 30 and pharmaceutical analysis 31,32 involving carboxylate bearing compounds.The aim of the present contribution was to develop a simple and low-cost potentiometric flufenamate ISE immobilized in a graphite matrix (Pt Hg Hg 2 (FF) 2 Graphite, where FF stands for flufenamate ion) and to evaluate the applicability of this sensor to the determination of FFA in dosage forms and human serum samples. …”

mentioning

confidence: 99%

Development of a potentiometric flufenamate ISE and its application to pharmaceutical and clinical analyses

Santini¹,

Pezza²,

Oliveira³

et al. 2008

J. Braz. Chem. Soc.

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As características, o desempenho e a aplicação de um eletrodo do tipo Pt Hg Hg 2 (FF) 2 Grafite, FF=íon flufenamato, são descritas. O eletrodo responde a FF com sensibilidade de (-58,6 1,2) mV década -1 no intervalo de 1,0 10 -6 -1,0 10 -2 mol L -1 , a pH 6,0 -9,0 e com um limite de detecção de 7,1 10 -7 mol L -1 . O eletrodo é de custo relativamente baixo e facilmente construído, apresenta um rápido tempo de resposta (10-25 s) e pode ser usado por um período de 5 meses sem qualquer variação considerável nas suas características de desempenho. O eletrodo proposto mostrou boa seletividade para o íon flufenamato na presença de várias substâncias, bem como na presença de alguns carboxilatos e ânions inorgânicos. O eletrodo foi aplicado com sucesso na determinação de ácido flufenâmico em medicamentos e amostras de soro humano.The characteristics, performance, and application of an electrode, namely, Pt Hg Hg 2 (FF) 2 Graphite, where FF stands for flufenamate ion, are described. This electrode responds to FF with sensitivity of (-58.6 1.2) mV decade -1 over the range 1.0 10 -6 -1.0 10 -2 mol L -1 at pH 6.0 -9.0 and a detection limit of 7.1 10 -7 mol L -1. The electrode is easily constructed at a relatively low cost with fast response time (within 10-25 s) and can be used for a period of 5 months without any considerable change in its performance characteristics. The proposed electrode displayed good selectivity for flufenamate in the presence of several substances as well as in the presence of some carboxylate and inorganic anions. The electrode was successfully applied to the determination of flufenamic acid in pharmaceuticals and human serum samples.Keywords: flufenamate-sensitive electrode, potentiometry, dosage forms, blood serum IntroductionFlufenamic acid (FFA), [N-( , , -trifluoro-m-tolyl) anthranilic acid] is an analgesic, anti-inflammatory and antipyretic drug. It is extensively used in the treatment of arthritis and other illnesses related to muscular-skeletal problems.1 However the use of this drug has been implicated in some cases of nephrotoxicity.2 Due to the importance of the assay of FFA for pharmaceutical formulations and biological fluids, several analytical methods have been developed for the quantitative determination of this drug in both pharmaceutical and biological samples.These methods include spectrophotometry, 21,22 However, many of these methods are often time-consuming, technically demanding and require the use of costly, highly specialized instruments. Thus, there is an important demand for simple, low-cost, sensitive and rapid alternative methods for the determination of FFA in pharmaceuticals and biological fluids.Potentiometric methods with ion-selective electrodes (ISE's) have proved to be effective for the analysis of pharmaceutical formulations and biological samples, because these sensors offer the advantages of simple design, construction, and manipulation, reasonable selectivity, fast response time, applicability to colored and turbid solutions and possible interfacing with au...

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“…τον όρο (D-1) V c -V s με Υ και τον όρο (cV s /F) με Α, τότε η σχέαη (Β 11) γίνεται•. y=:AC s(6)(7)(8)(9)(10)(11)(12) …”

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Εφαρμογες Των Εκλεκτικων Ηλεκτροδιων Σε Μελετες Αλληλεπιδρασης Διαφορων Ιοντων Με Την Αλβουμινη

Χριστόπουλος¹,

Christopoulos²

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Preparation of an ion-selective electrode sensitive to flufenamic acid anion and its application to the study of protein binding of flufenamate in aqueous solution.

Cited by 9 publications

References 0 publications

Binding studies using ion-selective electrodes. Examination of the picrate-albumin interaction as a model system

Binding studies using ion-selective electrodes. Examination of the picrate-albumin interaction as a model system

Development of a potentiometric flufenamate ISE and its application to pharmaceutical and clinical analyses

Εφαρμογες Των Εκλεκτικων Ηλεκτροδιων Σε Μελετες Αλληλεπιδρασης Διαφορων Ιοντων Με Την Αλβουμινη

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