Preparation of Ampicillin Surface Molecularly Imprinted Polymers for Its Selective Recognition of Ampicillin in Eggs Samples

Abstract

Surface-imprinted polymers (MIPs) microspheres with the ability to specifically recognize water-soluble molecules were prepared using self-made monodisperse porous poly(chloromethylstyrene-co-divinylbenzene) beads as the solid-phase matrix and ampicillin (AMP) as the template molecule. MIPs were synthesized using different template molecule: monomer: crosslinker ratios and the optimum preparation ratio were obtained by measuring adsorption. The maximum equilibrium amount of adsorption by the MIPs reached 115.62 mg/g. Scatchard analysis indicated that the MIPs contained two types of recognition sites: specific and nonspecific. Based on the adsorption kinetics, adsorption equilibrium was reached after 30 minutes. Penicillin G, amoxicillin, and sulbactam acid were used as competitive molecules to research the selective adsorption capacity of the MIPs. The imprinted material was found to have good selectivity with selectivity coefficients for penicillin G, amoxicillin, and sulbactam acid of 5.74, 6.83, and 7.25, respectively. The MIPs were used as solid-phase extraction filler, resulting in successful enrichment and separation of ampicillin residue from egg samples. Standard addition recovery experiments revealed that recovery was good with recoveries from the spiked samples ranging from 91.5 to 94.9% and relative standard deviations from 3.6 to 4.2%. The solid-phase extraction MIPs microcolumn was reused 10 times, where it maintained a recovery rate of over 80%. This work presents a sensitive, fast, and convenient method for the determination of trace ampicillin in food samples.

Molecularly imprinted polymers—A closer look at the control polymer used in determining the imprinting effect: A mini review

“…38 This difference in pore size has also been linked to the high specific surface area and binding amount observed for MIPs. 38,39 Furthermore, studies on surface morphology using SEM have shown remarkable difference between MIPs and NIPs, whereby MIPs have demonstrated a highly porous surface (Figure 3), which has been attributed to formation of selective cavities within the polymer matrix.…”

“…In terms of pore size distribution, there are reported differences between MIPs and NIPs at both the macropore and mesopore region, where MIPs display a higher population than NIPs, which has been attributed to the template effect (Figure 2). 38 This difference in pore size has also been linked to the high specific surface area and binding amount observed for MIPs 38,39 …”

Recent Advances in Molecularly Imprinted Polymers for Antibiotic Analysis

“…The applications of MISPE and MIP sensors for the analytical determination of antibiotic residues are comprehensively reviewed according to the different kinds of SAs, QNs, BALs, MALs, TCs, AGs and others. Some typical examples of MISPE in antibiotic analysis are listed in Table 1 [10,11,23,40,42,45,49,51,56,59,[78][79][80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95], and MIP-based electrochemical sensors and optical sensors for antibiotic analysis are shown in Table 2 [68][69][70]72,[96][97][98][99][100][101][102][103][104][105][106][107] and Table 3 [74,76,…”

Development of a MIP-Based QCM Sensor for Selective Detection of Penicillins in Aqueous Media

“…Amoxicillin (Amo) is a common aminopenicillin. Usually, one can obtain it semi-synthetically from natural penicillin [8]. To date, it is the most prescribed antibiotic for children [9,10].…”