Preparation of a poly(ethyleneimine) embedded phenyl stationary phase for mixed-mode liquid chromatography

Ren, Xiujun; Hu, Chengxia; Gao, Die; Fu, Qifeng; Zhang, Kailian; Zu, Fengjiao; Zeng, Jing; Wang, Lujun; Xia, Zhining

doi:10.1016/j.aca.2018.09.049

Cited by 33 publications

(6 citation statements)

References 48 publications

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“…The activated silica and MPTMS‐SiO 2 were prepared according to the reported work and , respectively. The RPS stationary phase was synthesized as follows: 86 mg (1 mmol) MAA, 574 mg (1 mmol) EGMRA, and 2 mg AIBN were dissolved in 5 g of chloroform and subjected to ultrasonication for 5–10 min.…”

Section: Methodsmentioning

confidence: 99%

Alkaloid purification using rosin‐based polymer‐bonded silica stationary phase in HPLC

Sun

Wang

et al. 2019

J of Separation Science

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Alkaloids are important natural products that exhibit a wide spectrum of pharmacological activities. To efficiently separate and purify them, a rosin‐based polymer‐bonded silica stationary phase in high‐performance liquid chromatography was synthesized via the surface radical polymerization of ethylene glycol maleic rosinate acrylate and methacrylic acid onto functionalized silica. The stationary phases, columns, optimization of chromatographic conditions for alkaloids, and thermodynamic behavior of the analytes on the column were fully studied. Under the optimized conditions, the prepared column efficiently purified natural camptothecine, caffeine, and evodiamine with the corresponding purities of 92, 96, and 97%. With this work, we have developed an efficient approach to isolate alkaloids and promoted the research on rosin‐based materials in biomedicine and analytical chemistry.

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Section: Methodsmentioning

confidence: 99%

Alkaloid purification using rosin‐based polymer‐bonded silica stationary phase in HPLC

Sun

Wang

et al. 2019

J of Separation Science

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“…Electrostatic interactions were observed with imidazolium cations and carboxylate anions. Similar ideas were applied to the preparation of an IL modified with C18 and acryl sulfonate (R = C18 or acryal sulfonate) on silica support (Ren et al, 2018). Most IL-based stationary phases are prepared by modifying the IL with various functional groups and then bonding the modified IL to the silica surface.…”

Section: Rplc/hilic Mixed Modementioning

confidence: 99%

“…Interlinked triphenylbenzene and triazene imparted both hydrophobic and hydrophilic properties to the stationary phase. to produce a PEI-embedded mixed-mode stationary phase (Ren et al, 2018;Wan et al, 2020). This modification provided phenyl moiety to the PEI polymer.…”

Section: Rplc/hilic Mixed Modementioning

confidence: 99%

“…The properties of this stationary phase were tunable by controlling the ratio of the three monomers and could influence the retention and selectivity in both RPLC and HILIC modes. PEI bonded to the silica support has been modified with glycidylphenylether and N ‐acetyl‐ l ‐phenylalanine to produce a PEI‐embedded mixed‐mode stationary phase (Ren et al, 2018; Wan et al, 2020). This modification provided phenyl moiety to the PEI polymer.…”

Section: Polar Stationary Phases For Hilicmentioning

confidence: 99%

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A survey of polar stationary phases for hydrophilic interaction chromatography and recent progress in understanding retention and selectivity

Guo

2022

Biomedical Chromatography

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Various polar stationary phases are available for hydrophilic interaction chromatography (HILIC) and help drive continuous applications in biomedical, environmental, and pharmaceutical areas in the past decade. Although the stationary phases for HILIC have been reviewed previously, it is an appropriate time to take another look at the progresses made during the past 5 years. The current review provides an overview of the polar stationary phases commercially available for HILIC applications in an effort to assist scientists in selecting suitable columns. New types of stationary phases that were published in literature in the past 5 years are summarized and discussed. The trend in stationary phase research and development is also highlighted. Of particular interest is the experimental evidence for direct interactions of polar analytes with the ligands of the stationary phases under HILIC conditions. In addition, two different approaches have been developed to delineate the relative significance of the partitioning and adsorption mechanisms in HILIC, representing an important advancement in our understanding of the retention mechanisms in HILIC.

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“…As the heart of high-performance liquid chromatography (HPLC) systems, stationary phases have drawn extensive attention. To meet the growing demand in different fields, various stationary phases used for HPLC have been developed. − Recently, stationary phases with mixed-mode mechanisms have emerged as the hot research topic, owing to the advantages of much higher sample loading capability, high selectivity, and good separation efficiency over the classical single-mode stationary phases. − Up to now, many kinds of mixed-mode stationary phases, including ion exchange chromatography (IEC)/reversed-phase liquid chromatography (RPLC), − hydrophilic interaction liquid chromatography (HILIC)/RPLC, ,, HILIC/IEC − and RPLC/HILIC/IEC, , were reported and applied in a number of separation fields. However, the search for multifarious stationary phases is still a growing demand in order to resolve the tasks of ever-evolving actual applications.…”

Section: Introductionmentioning

confidence: 99%

Core–Shell Metal–Organic Frameworks as the Mixed-Mode Stationary Phase for Hydrophilic Interaction/Reversed-Phase Chromatography

Liu

et al. 2019

ACS Appl. Mater. Interfaces

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Stationary phases with mixed-mode mechanisms have emerged as a hot research topic. In the present research, monodisperse core−shell UiO-67@SiO 2 materials were prepared and further served as the packed column for mixed-mode hydrophilic interaction liquid chromatography/reversed-phase liquid chromatography. The developed UiO-67@SiO 2 materials were characterized via thermogravimetric analysis, scanning electron microscopy, X-ray Powder diffraction, and Fourier transform infrared techniques. The developed UiO-67@SiO 2 column shows flexible selectivity for separation of both hydrophobic (anilines, alkylbenzenes, and polycyclic aromatic hydrocarbons) and hydrophilic (thioureas) compounds. Furthermore, the UiO-67@SiO 2 column was also utilized to characterize potential pollutants in lake water samples. In summary, the UiO-67@SiO 2 column provided flexible selectivity and wide-range retention behaviors for both hydrophilic and hydrophobic analytes.

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Preparation of a poly(ethyleneimine) embedded phenyl stationary phase for mixed-mode liquid chromatography

Cited by 33 publications

References 48 publications

Alkaloid purification using rosin‐based polymer‐bonded silica stationary phase in HPLC

Alkaloid purification using rosin‐based polymer‐bonded silica stationary phase in HPLC

A survey of polar stationary phases for hydrophilic interaction chromatography and recent progress in understanding retention and selectivity

Core–Shell Metal–Organic Frameworks as the Mixed-Mode Stationary Phase for Hydrophilic Interaction/Reversed-Phase Chromatography

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