Preparation of a novel molecularly imprinted polymer by the sol–gel process for solid phase extraction of vitamin D3

Abstract

A novel molecularly imprinted polymer (MIP) as an artificial receptor for vitamin D3 is prepared and used. A sol-gel process is used to prepare the hybrid MIP. For organic part (gel) of MIP, methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA) and benzoylperoxide are used as functional monomer, cross-liker and initializer, respectively. As inorganic part (sol) of the MIP, tetraethyl orthosilicat (TEOS) is used. Main parameters affecting synthesis of organicinorganic hybrid MIP are investigated and optimized. The imprinting efficiency of MIP is evaluated by comparing the adsorbed amount of vitamin D3 by MIP with that by the related non-imprinted polymer (NIP). Betamethasone, dexamethasone, 17-beta-stradiol and 25-hydroxy vitamin D3 were chosen to study the selectivity of the MIP. This study revealed that the MIP possesses a remarkable affinity for vitamin D3. The limit of detection has been gained 1 ng/ml. The linear range and relative standard deviation (RSD %) were 1-10000 ng/mL and 4 (n = 3), respectively. The hybrid MIP provides a fast, selective and convenience separation of vitamin D3 from aqueous samples.

Towards Clean and Safe Water: A Review on the Emerging Role of Imprinted Polymer-Based Electrochemical Sensors

“…Three reaction steps are generally involved during a given sol-gel procedure: (i) interaction between metal cations and water molecules, (ii) hydrolysis of silicone monomers, and (iii) polycondensation of the silica into a porous 3D network. Sol-gel chemistry provides a relatively simple way for the design of electrochemical sensing layers [ 50 , 51 , 52 ]. A schematic representation of sol-gel methods is depicted in Figure 5 .…”

An Overview of Molecularly Imprinted Polymers Embedded with Quantum Dots and Their Implementation as an Alternative Approach for Extraction and Detection of Crocin

“…Furthermore, in MIPs made by sol-gel technology, extracting the template molecule from the polymer is more efficient. [91][92][93][94][95] The Stöber method and the reverse phase microemulsion method are the two main methods for creating MIP-QDs sensors using the sol-gel approach. The synthesis of tetraethyl orthosilicate (TEOS) and [3(methacryloxy) propyl] trimethoxy silane is one of the applications of silica-based QDs.…”

The Importance of Developing Electrochemical Sensors Based on Molecularly Imprinted Polymers for a Rapid Detection of Antioxidants

“…Some of the applications of MIS in analytical and bioanalytical fields are solid-phase extraction of enrofloxacin from fish and chips samples [ 174 ], methyl-3-quinoxaline-2-carboxylic acid and quinoxaline-2-carboxylic acid from pork muscle [ 175 ], florfenicol from meat samples [ 176 ], polar organophosphorus pesticides from almond oil [ 177 ], iprodione fungicide from wine samples [ 167 ], methylxanthines from natural water and human urine [ 172 ], patulin from apple juice samples [ 178 ], vitamin D3 from aqueous samples [ 179 ], phenobarbital in human plasma [ 165 ], solid-phase microextraction of fentanyl [ 180 ] and bilirubin [ 181 ] from urine and plasma samples, etc.…”