Preparation of a Monoclonal Antibody to Nϵ-(Hexanonyl)lysine: Application to the Evaluation of Protective Effects of Flavonoid Supplementation against Exercise-Induced Oxidative Stress in Rat Skeletal Muscle

Kato, Yoji; Miyake, Yoichi; Yamamoto, Kanefumi; Shimomura, Yoshiharu; Ochi, Hirotomo; Mori, Yoko; Osawa, Toshihiko

doi:10.1006/bbrc.2000.3150

Cited by 65 publications

(47 citation statements)

References 25 publications

(32 reference statements)

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“…Monoclonal antibodies and ELISA kits have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It has been also reported that HEL is formed in rat muscle during exercise and that its formation is inhibited by antioxidants such as flavonoids [169].…”

Section: Early Stage Of Lipid Peroxidation Productsmentioning

confidence: 96%

Lipid Peroxidation and Antioxidants in Arterial Hypertension

Sousa¹,

Afonso²,

Albino‐Teixeira³

et al. 2012

Lipid Peroxidation

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Section: Early Stage Of Lipid Peroxidation Productsmentioning

confidence: 96%

Lipid Peroxidation and Antioxidants in Arterial Hypertension

Sousa¹,

Afonso²,

Albino‐Teixeira³

et al. 2012

Lipid Peroxidation

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“…Oxidative damage of lipids caused by reactive oxygen species (ROS) plays an important role in inflammatory diseases. The HEL is one of the biomarkers for oxidative stress [21]. The HEL moiety is generated from the reaction of lipid hydroperoxidase with protein and can be detected by the ELISA kit.…”

Section: Determination Of Salivary 8-hydroxydeoxyguanosine and N-(hementioning

confidence: 99%

Ability of Orally Administered IFN-.ALPHA.4 to Inhibit Naturally Occurring Gingival Inflammation in Dogs

Ito

Isogai

Yoshioka

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. It has been reported that type I interferons (IFN-) play an important role in innate immune responses against viral and bacterial infections. In this study, we used and examined naturally occurred canine periodontal disease to show the therapeutic efficacy of low dose oral administration (LDOA) of canine IFN- subtype 4 (CaIFN-4). We administered purified recombinant CaIFN-4 expressed in a baculovirus system to dogs with or without gingival inflammation. We found that LDOA of CaIFN-4 reduce periodontopathic bactrerial counts. LDOA induced improvement of naturally occurring gingival inflammation, and reduction of the stress marker responses was also observed after LDOA. These results suggest that LDOA of CaIFN-4 has effectiveness for improvement of naturally occurring gingival inflammation in dogs.

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“…It was thought that LP and FLP have a suppressive effect against the oxidative damage of lipid, which is caused by exercise, and that the effect of FLP has a higher tendency than that of LP. HEL is reported to be a biomarker of oxidative stress for the early stage of lipid peroxidation because it is formed by the reaction between the hydroperoxide caused from linoleic acid and lysine (Kato et al, 2000). Therefore, HEL is thought to have a high sensitivity for measuring the oxidative damage of lipids in comparison with TBARS, which are end products of lipid peroxide.…”

Section: Resultsmentioning

confidence: 99%

“…8-Hydroxyhesperetin, 6-hydroxynaringenin and 8-hydroxynaringenin were prepared by the method of . Anti-N e -(hexanonyl)lysine (HEL) monoclonal antibody prepared as described previously (Kato et al, 2000) of Applied Microbiology at Tokyo University (Tokyo). Other reagents were purchased from Wako Chemical, Ltd., Osaka, Japan.…”

Section: Methodsmentioning

confidence: 99%

“…The homogenate was centrifuged at 4500ϫg for 15 min, and the supernatant obtained was used for the following biochemical measurement. The noncompetitive ELISA was performed for measurement of HEL of oxidative stress marker as described previously (Kato et al, 2000). Briefly, 50 l of sample solution (10 g protein/ml) was pipetted into the wells and kept at 4˚C overnight.…”

Section: Measurement Of Hel Content By Enzyme-linked Immunosorbent Asmentioning

confidence: 99%

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Radical-Scavenging Activity in vitro of Lemon Peel Fermented with Aspergillus saitoi and Its Suppressive Effect against Exercise-Induced Oxidative Damage in Rat Liver

Miyake

Minato

Fukumoto³

et al. 2004

FSTR

Self Cite

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We prepared fermented lemon peel (FLP) with high antioxidative activity by fermentation of the lemon peel (LP) with Aspergillus saitoi. The antioxidative activity of LP and FLP was examined by the radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) in vitro, and the suppressive effect against exercise-induced oxidative damage in rat liver. FLP exhibited higher radical-scavenging activity against DPPH than LP. The administration of LP and FLP prior to exercise significantly suppressed the increases in thiobarbituric acid-reactive substance caused by lipid peroxidation during exhaustive exercise, with FLP having a tendency of higher activity than LP. Moreover, FLP showed a significant suppressive effect of N e -(hexanonyl)lysine content, a primary oxidative stress marker, which increases due to exhaustive exercise, whereas LP did not. The fermentation of LP with A. saitoi was thought to produce a high antioxidative activity. The high antioxidative activity of FLP was assumed to be related to the production of antioxidative flavonoids, as a hydroxyflavanone and aglycones, by the fermentation of lemon peel with Aspergillus saitoi.

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Preparation of a Monoclonal Antibody to Nϵ-(Hexanonyl)lysine: Application to the Evaluation of Protective Effects of Flavonoid Supplementation against Exercise-Induced Oxidative Stress in Rat Skeletal Muscle

Cited by 65 publications

References 25 publications

Lipid Peroxidation and Antioxidants in Arterial Hypertension

Lipid Peroxidation and Antioxidants in Arterial Hypertension

Ability of Orally Administered IFN-.ALPHA.4 to Inhibit Naturally Occurring Gingival Inflammation in Dogs

Radical-Scavenging Activity in vitro of Lemon Peel Fermented with Aspergillus saitoi and Its Suppressive Effect against Exercise-Induced Oxidative Damage in Rat Liver

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