Preparation of a dextran sulfate functionalized monolithic silica column for hydrophilic interaction/cation exchange chromatography

Yang, Peiling; Zhou, Qilai; Li, Jia

doi:10.1039/c3ay40180d

Cited by 7 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Small size monolithic silica capillary columns can be prepared and modified in a meter long tube that could provide a high efficiency HILIC column generating 300 000 theoretical plate with a 200 cm column . Monolithic silica capillary columns of good performance can be prepared using information available in the literature and successfully modified while maintaining the column efficiency, as shown in several cited references, for the preparation of reversed-phase, HILIC, − affinity, and mixed-mode stationary phases. Successful modification of rod columns was also reported for reversed-phase and chiral stationary phases. ,, Chemical stability and high-temperature stability of the cladding material should be taken into account for rod columns.…”

Section: Monolithic Silica Columnsmentioning

confidence: 99%

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Tanaka¹,

McCalley

2015

Anal. Chem.

151

View full text Add to dashboard Cite

Section: Monolithic Silica Columnsmentioning

confidence: 99%

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Tanaka¹,

McCalley

2015

Anal. Chem.

151

View full text Add to dashboard Cite

“…The optimal H was 10 μm at u = 0.6 mm/s, which was better than the corresponding APTES column. The column showed large retention (k = 20-22) for amino acids in 90% acetonitrile (ACN) (89). Preparation and column efficiency of HILIC monolithic silica columns are summarized in Table 2. 3.2.4.…”

Section: Hydrophilic Interaction Liquid Chromatographymentioning

confidence: 99%

Recent Progress in Monolithic Silica Columns for High-Speed and High-Selectivity Separations

Ikegami

Tanaka²

2016

Annual Rev. Anal. Chem.

View full text Add to dashboard Cite

Monolithic silica columns have greater (through-pore size)/(skeleton size) ratios than particulate columns and fixed support structures in a column for chemical modification, resulting in high-efficiency columns and stationary phases. This review looks at how the size range of monolithic silica columns has been expanded, how high-efficiency monolithic silica columns have been realized, and how various methods of silica surface functionalization, leading to selective stationary phases, have been developed on monolithic silica supports, and provides information on the current status of these columns. Also discussed are the practical aspects of monolithic silica columns, including how their versatility can be improved by the preparation of small-sized structural features (sub-micron) and columns (1 mm ID or smaller) and by optimizing reaction conditions for in situ chemical modification with various restrictions, with an emphasis on recent research results for both topics.

show abstract

“…In recent years, monolithic columns have become one of the focuses in separation science due to their high permeability, fast mass transfer, absence of end frits, and so on . With different chemical modification, monolithic columns can be applied in various separation modes . So far, mixed‐mode chromatography is more flexible than single‐mode chromatography since it combines the advantages of two or more kinds of separation modes together and reduces the overconsumption of materials and production of wastes .…”

Section: Introductionmentioning

confidence: 99%

Hydrothermal preparation of hybrid carbon/silica monolithic capillary column for liquid chromatography

et al. 2014

Self Cite

View full text Add to dashboard Cite

A simple, easy and economical approach for the preparation of a hybrid carbon/silica monolithic capillary column was described for the first time by using silica monolith as framework in combination with hydrothermal carbonization at 180°C. During the preparation process, formamide was introduced to the reaction solutions to reduce the dissolution rate of monolithic silica skeleton and its optimal concentration was 1.5 M. Fourier transform infrared spectrometry, scanning electron microscopy, energy dispersive X-ray spectrometry, and inverse size exclusion chromatography were carried out to characterize the as-prepared column. The results demonstrated that carbon spheres ranging from 150 to 1000 nm were successfully attached to the surface of silica skeleton. The prepared hybrid carbon/silica column had a permeability of 4.4 × 10(-14) m(2). Chromatographic performance of the column was evaluated by separation of various compounds including alkylbenzenes, nucleosides and bases, and aromatic acids. The column exhibited an efficiency of 75,000 plates/m for butylbenzene at the optimal linear velocity of 0.23 mm/s. The successful separation of these compounds and the study on mechanism indicated that the column can be applied in mixed-mode chromatography.

show abstract

Preparation of a dextran sulfate functionalized monolithic silica column for hydrophilic interaction/cation exchange chromatography

Cited by 7 publications

References 30 publications

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

Recent Progress in Monolithic Silica Columns for High-Speed and High-Selectivity Separations

Hydrothermal preparation of hybrid carbon/silica monolithic capillary column for liquid chromatography

Contact Info

Product

Resources

About