Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

Supporting Materials That Improve the Stability of Enzyme Membranes

“…For 100 days after preparing the membrane, the response magnitude remained almost unchanged; it gradually decreased after 120 days, 41 and reached 50% of the initial value after 300 days (data not shown here). Thus, the enzyme membrane improved the stability.…”

“…Fig. 3 Force modulation microscopy image of the cellulose membrane; 41 force modulation microscopy (FMM) phase image (500 nm × 500 nm) of the membrane. FMM was performed by vibrating the cantilever at 10 -300 kHz on atomic force microscopy.…”

“…A cellulose-containing ionic liquid was dropped on the dried enzyme layer; the ionic liquid was removed by immersing the whole plate in water. 41 After drying, a cellulose-covered enzyme layer was obtained.…”

Application of Enzyme Biosensors in Analysis of Food and Beverages

“…For the L-phenylalanine, the lower detection limit was 5 μM, and a linear response range was up to 1 mM with a response time of 40 s. Response to other amino acids, such as L-leucine and L-methionine was almost of the same magnitude as the one for Lphenylalanine. These results indicate that the electrode could be used for the L -amino acid detection such as Lphenylalanine, L-leucine, and L-methionine (Yabuki et al 2001). Basu et al developed a monosodium glutamate (MSG) biosensor made by co-immobilizing L-glutamate oxidase and L-glutamate dehydrogenase (LGLDH).…”

How to Lengthen the Long-Term Stability of Enzyme Membranes: Trends and Strategies

“…Another method to prevent enzyme loss to the substrates is by covering it with a density membrane (Figure 3). Nafion [77], chitosan [78], ionic polymers [79], polyion complex membranes [80][81][82][83], and cellulose [10,84,85] have been used to cover the enzyme layer. These methods are effective, but the resulting membranes can become thicker, i.e., penetration of substrates can worsen.…”