Preparation for aflatoxin B1-cationized bovine serum albumin based on Mannich-type reaction

Zhou, Youxiang; Wu, Jiang‐Lun; Yu, Wei; Xu, Ying; Wang, Ping; Xie, Bijun; Chen, Fusheng

doi:10.1016/j.jim.2007.08.009

Cited by 32 publications

(34 citation statements)

References 30 publications

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“…Goat anti-mouse IgG-HRP conjugates were from Sino-American Biotechnology Company (Beijing, China). AFB 1 O-BSA (n AFB1O : n BSA ¼ 4.88:1) and antiserum against AFB 1 were prepared according to the method described by Chu and Ueno (1977) and Zhou et al (2007). All other chemicals used in the present study were either analytical pure or with higher quality.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of latex agglutination inhibition reaction test for rapid detection of aflatoxin B1

Wang

Zhou³

et al. 2011

Food Control

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Section: Methodsmentioning

confidence: 99%

“…The procedure of indirect ELISA was the same as described previously by Zhou et al (2007). Briefly, 100 mL of coating antigen (2 mg/mL) AFB 1 O-BSA in bicarbonate buffer (pH 9.6) was added to each plate hole and incubated at 4 C overnight with relative humidity.…”

Section: Afb 1 Determination Of Samples By Indirect Competitive Elisamentioning

confidence: 99%

Evaluation of latex agglutination inhibition reaction test for rapid detection of aflatoxin B1

Wang

Zhou³

et al. 2011

Food Control

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“…[30]. The mycotoxin (~ 1 µmol) was dissolved in 1 mL of 0.1 M MES buffer (pH 4.8, containing 100 µL of DMFA) and added dropwise to cBSA solution (0.025 µmol).…”

Section: Synthesis Of Afb1-cbsa and Afm1-cbsamentioning

confidence: 99%

Liposomes loaded with quantum dots for ultrasensitive on-site determination of aflatoxin M1 in milk products

et al. 2013

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A quantitative fluorescence labeled immunosorbent assay (FLISA) and qualitative onsite column tests were developed for the determination of aflatoxin M1 (AfM1) in milk products. The use of quantum dots (QD) loaded liposomes as a label significantly increased the assay sensitivity by encapsulating multiple QD in a single liposome and, therefore, amplifying the analytical signal.Two different techniques were compared to obtain aflatoxin-protein conjugates, used for further coupling with the liposomes. The influence of non-specific interactions of the obtained liposomes-labeled conjugates with the surface of microtiter plates and column cartridges was evaluated and discussed. The limit of detection for FLISA was 0.014 µg kg -1 .For qualitative on-site tests the cut-off was set at 0.05 µg kg -1 taking into account the EU maximum level for aflatoxin M1 in raw milk, heat-treated milk and milk for the manufacture of milk based product. The direct addition of labeled conjugate in the milk samples resulted in an additional decrease of analysis time.An intra-laboratory validation was performed with sterilized milk and cream samples artificially spiked with AfM1 at concentrations less, equal and more than the cut-off level. It was shown that milk products could be analyzed without any sample preparation, just diluted with the buffer. The rates for false positive and false negative results were below 5% (2.6% and 3.3%, respectively).

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“…The emulsion solutions were injected intraperitoneally into BALB/c mice (No. 1-3) [23]. The subsequent three immunizations were given by intraperitoneal injections with the same quantity of immunogen emulsified with the incomplete Freund's adjuvant at 2-week intervals.…”

Section: Immunizationmentioning

confidence: 99%

“…The subsequent steps were the same as in "Determination of Polyclonal Antibody Level in Mice Serum by Indirect ELISA". Fifty percent inhibitory concentration (IC 50 ) was defined as the sensitivity of antibody [23].…”

Section: Determination Of the Sensitivity Of Monoclonal Antibodymentioning

confidence: 99%

Production of a Monoclonal Antibody by Simultaneous Immunization of Staphylococcal Enterotoxin A and B

Liang

Zhang

Liu

et al. 2011

Appl Biochem Biotechnol

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In this paper, a method of simultaneous immunizing BALB/c mice with staphylococcal enterotoxin (SE) A and B (SEA and SEB) to prepare a monoclonal antibody (3F2) for detecting both of SEA and SEB was developed. The results showed that antibody 3F2 had high titers against both SEA and SEB by enzyme-linked immunosorbent assay (ELISA). The sensitivities of 3F2 to SEA and SEB detected by ELISA were 133.2 and 82.5 ng/mL, respectively, and the detection limits for the two enterotoxins were about 1 ng/mL. The antibody 3F2 had high specificities and affinities to both SEA and SEB, and had no cross-reaction with SEC(1), bovine serum albumin, and ovalbumin. SEs-free skimmed milk samples were spiked with different concentrations of SEA, SEB, or both of them, respectively. Average recoveries of SEA and SEB from the spiked samples were all nearly between 82% and 104%. The result suggested that one cell fusion with simultaneous immunization by multiple antigen to prepare monoclonal antibody against them was possible, simple, and economic. The monoclonal antibody could be used in simultaneous detecting multifarious SEs.

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Preparation for aflatoxin B1-cationized bovine serum albumin based on Mannich-type reaction

Cited by 32 publications

References 30 publications

Evaluation of latex agglutination inhibition reaction test for rapid detection of aflatoxin B1

Evaluation of latex agglutination inhibition reaction test for rapid detection of aflatoxin B1

Liposomes loaded with quantum dots for ultrasensitive on-site determination of aflatoxin M1 in milk products

Production of a Monoclonal Antibody by Simultaneous Immunization of Staphylococcal Enterotoxin A and B

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