Preparation Fe3O4@chitosan magnetic particles for covalent immobilization of lipase from Thermomyces lanuginosus

Wang, Xiangyu; Jiang, Xiangping; Li, Yue; Zeng, Sha; Zhang, Ye-Wang

doi:10.1016/j.ijbiomac.2015.01.020

Cited by 115 publications

(39 citation statements)

References 42 publications

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“…The properties of the carrier play an important role and normally the carrier should have large surface area [13][14][15]. Graphene with a lamellar structure has many industrial applications due to its unique physical and electronic properties [16].…”

Section: Introductionmentioning

confidence: 99%

Rapid Immobilization of Cellulase onto Graphene Oxide with a Hydrophobic Spacer

Gao

Wang

et al. 2018

Catalysts

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A rapid immobilization method for cellulase was developed. Functional graphene oxide was synthesized and grafted with hydrophobic spacer P-β-sulfuric acid ester ethyl sulfone aniline (SESA) though etherification and diazotization. The functionalized graphene oxide was characterized by Fourier-transform infrared spectroscopy and was used as the carrier for the immobilization of cellulase via covalent binding. The immobilization of cellulase was finished in a very short time (10 min) and very high immobilization yield and efficiency of above 90% were achieved after optimization. When compared with the free cellulase, thermal and operational stabilities of the immobilized cellulase were improved significantly. At 50 °C, the half-life of the immobilized cellulase (533 min) was six-fold higher than that of the free cellulase (89 min). Additionally, the affinity between immobilized cellulase (Km = 2.19 g L −1 ) and substrate was more favorable than that of free cellulase (Km = 3.84 g L −1 ), suggesting the immobilized cellulase has higher catalytic efficiency. The possible immobilization mechanism was proposed. The results strongly indicate that the immobilization is highly efficient and has great potential for the immobilization of other enzymes.

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Section: Introductionmentioning

confidence: 99%

Rapid Immobilization of Cellulase onto Graphene Oxide with a Hydrophobic Spacer

Gao

Wang

et al. 2018

Catalysts

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“…CS has been proved to have many other intrinsic properties, such as low toxicity (lethal dose 50% in mice is 16 g/kg body weight) [2], biocompatibility and biodegradability (Cordova et al, 2008). It is mucoadhesive polymer and can increase the residence time at the site of absorption and has favorable controlled drug-release abilities [3]. It has been demonstrated that CS (when protonated) affects cell permeability and enhances paracellular permeability of drugs across the mucosal epithelia by opening the intercellular tight junctions.…”

Section: Introductionmentioning

confidence: 99%

Formulation Optimization and Characterization of Ganciclovir Loaded Dry Chitosan Nanoparticles

Patel

Gajra

et al. 2017

Asian J Pharm Clin Res

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Objective: The objective of this work was to formulate, optimize, and characterize ganciclovir (GCV) loaded dry chitosan nanoparticles (CSNPs). Methods:The GCV loaded CSNPs was prepared by ionic gelation method. Box-Behnken design was employed to optimize the influence of independent process and formulation variables like drug to polymer ratio, concentration of sodium tripolyphosphate, and stirring time (min) on the dependent variables such as particle size (PS) and drug encapsulation efficiency (% EE). The optimum conditions were determined by regression analysis of the output data. Results:The independent variables had interactive effects and they affected both the responses. The optimum formulation had PS within the range of 100-120 nm and % EE between 85% and 86%. The prepared GCV loaded CSNPs were dried by fluidized bed drying method. Fourier transform infrared spectra showed there was no physicochemical interaction between GCV and CS. Powder X-ray diffraction study showed less intense crystalline peaks indicated that GCV may exist in the formulation as amorphous nanodispersion or molecular dispersion form. Differential scanning calorimetry study was performed which indicated that the drug was molecularly dispersed inside the matrix of CS. Higuchi model was the best to fit the in vitro release data for the GCV loaded CSNPs. Conclusion:From the results, it can be concluded that the GCV loaded dry CSNPs were formulated, optimized, and characterized using desired pharmacotechnical properties.

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“…Therefore, there is still a high scope of finding new matrices or immobilization methods for GGT enzyme immobilization. Chitosan has been used for enzyme immobilization in different forms and for various enzymes [9,15,[18][19][20][30][31][32]. It has also been used as micro or nano-carriers for bioactive molecules [12,13,16,17].…”

Section: Immobilization Of Blggt On Chitosan Microspheresmentioning

confidence: 99%

“…In addition to facilitating enzyme recycling, it aids in downstream processing of the products [8]. Although, on the downside, immobilization of a biocatalyst onto a solid surface or matrix may limit its flexibility and substrate accessibility [9,10]. However, this is overshadowed by a massive improvement in enzyme stability and storability [11].…”

Section: Introductionmentioning

confidence: 99%