Preparation, evaluation, and application of dummy molecularly imprinted polymer for analysis of hesperidin in lime juice

Abstract

Lime juice as the most commonly used natural source production can be characterized using determination of flavonoids contents such as hesperidin. So, development of analyzing methods for checking the quality and healthiness of lime juices is necessary. In this study, we aimed to set up a selective solid phase extraction method using dummy molecularly imprinting approach for extraction and separation of hesperidin in lime juice to check the quality of commercial lime juice products of Mashhad city market. The imprinted polymers were synthesized by hesperitin as dummy template due to the hesperitin solubility in the wide range of porogenic solvents. The specificity extent of synthesized polymers toward hesperidin was tested and optimum one was used as adsorbent in solid‐phase extraction cartridge. The dummy molecularly imprinted polymer with high adsorption capacity for hesperidin (dissociation constant 0.12 μM) was successfully used for extraction and clean‐up of hesperidin in the lime juice matrix prior to analysis by high‐performance liquid chromatography. The analysis of hesperidin was done in the range of 0.312–50 μg/mL with detection limit of 0.05 μg/mL. This technique was successfully set up to remove the interfering compounds for analysis of hesperidin in commercial lime juice products.

A new molecularly imprinted polymer electrochemical sensor based on CuCo2O4/N‐doped CNTs/P‐doped GO nanocomposite for detection of 25‐hydroxyvitamin D3 in serum samples

“…Based on the Langmuir model, the binding isotherm (Figure 6D), which was not a straight line, demonstrated the saturation presence in the high concentration range. As shown in the Scatchard plot (Figure 6E), the presence of curvature demonstrated the creation of two kinds of high‐ and low‐affinity interaction sites due to complete and incomplete interactions between 25(OH)D 3 and PPy, 71 respectively. According to Equation (3), K A values were obtained 2 × 10 8 M –1 and 1 × 10 7 M –1 for high‐ and low‐affinity binding sites, respectively.…”

Preparation and Application of Molecularly Imprinted Polymers for Flavonoids: Review and Perspective

“…However, the reaction system of precipitation polymerization is simpler in several aspects: there is no need for surfactant and other solvents, there is less chance of losing the functional monomer, and it is much easier to control the polymer particle size [ 34 , 35 ]. Recently, the synthesis of MIPs using precipitation polymerization has been widely reported [ 36 , 37 ]. In a typical synthesis, the imprinting system is dissolved in the corresponding solvent, and the polymers insoluble in the reaction system are obtained after polymerization reaction, and then the polymers are collected by centrifugation or filtration, etc.…”

Application of molecularly imprinted solid‐phase extraction coupled with liquid chromatography method for detection of penicillin G in pasteurised milk samples

“…2013; Mohammadinejad et al . 2021):where ‘Bound’ and ‘Free’ are defined as the amounts of Pen G bound and unbound to the polymer, respectively (at equilibrium conditions). B max is the maximum number of binding sites and K D is the dissociation constant.…”