Application of molecularly imprinted solid‐phase extraction coupled with liquid chromatography method for detection of penicillin G in pasteurised milk samples

Abstract

In the present study, a polymeric powder, synthesised using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker, was used as a highly selective sorbent in a molecularly imprinted solid-phase extraction cartridge to extract penicillin G from milk samples. Detection was performed using high performance liquid chromatography. The proposed method was used to determine penicillin G in the milk matrix in the range of 3-1000 ng/mL (limit of detection 2 ng/mL) with relative recoveries of 81-90% (relative standard deviation 2.14-4.35%). Finally, the calibrated method was used to quantify penicillin G residue in commercial pasteurised milk samples.

Recent Advances in Molecularly Imprinted Polymers for Antibiotic Analysis

“…The applications of MISPE and MIP sensors for the analytical determination of antibiotic residues are comprehensively reviewed according to the different kinds of SAs, QNs, BALs, MALs, TCs, AGs and others. Some typical examples of MISPE in antibiotic analysis are listed in Table 1 [10,11,23,40,42,45,49,51,56,59,[78][79][80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95], and MIP-based electrochemical sensors and optical sensors for antibiotic analysis are shown in Table 2 [68][69][70]72,[96][97][98][99][100][101][102][103][104][105][106][107] and Table 3 [74,76,…”

“…Pourtaghi et al [80] prepared MIPs using penicillin G (PEN-G) as a template, MAA as a functional monomer and EGDMA as a cross-linker, as an adsorbent for SPE, combined with HPLC-UV, to detect PEN-G in milk samples. The results showed that the LOD was 2 ng/mL and the relative recoveries were 81-90%.…”

“…It has been successfully applied to commercial sterilized milk samples for the detection of PEN-G residues. Others also reported the determination of BALs using MISPE combined with LC-MS/MS [80][81][82][83].…”

Antibiotic Residues of Penicillin and Oxytetracycline in Yoghurt: One Health Needs Paradigm Shift, Legislation and Policy Formulation in Pakistan

“…The first antibiotic to be utilized in veterinary medication was penicillin G, which was at first made accessible in 1947 (Samad, 2022). Misuse of this antibiotics may led to the resistance and residues in dairy products (Pourtaghi et al, 2022). Poor quality milk containing the ABs poses threat to the one health and public health.…”