Preparation, characterization, and preliminary biocompatibility evaluation of particulate spin-coated mesoporous silica films

Wiltschka, Oliver; Böcking, Dominique; Miller, Larissa; Brenner, Rolf E.; Sahlgren, Cecilia; Lindén, Mika

doi:10.1016/j.micromeso.2014.01.006

Cited by 19 publications

(19 citation statements)

References 37 publications

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“…Previous studies have reported that a cellular uptake of hydrophobic drugs is favored by intracellular release of drugs from loaded particles taken up from the lm surface. 20 The possibility of particle-mediated transport of drugs was investigated by further cell experiments. Therefore, cells were seeded on the surface of DiG_0.00 lms loaded with the model drug DiO and the uptake of drug and particles was investigated by CLSM.…”

Section: Model Drug Loading and Releasementioning

confidence: 99%

“…This can be compared to the spin-coated particulate system developed by Wiltschka et al that showed a signicantly increased uptake of particles by the cells and particle-free areas were easily observed around the adhered cells. 20 A consequence of an increased particle uptake is a non-desired increased cell toxicity as shown by Böcking et al, who observed an enhanced toxicity when more than 5 layers of particles were spin-coated onto the substrate. 38 In addition, a long-lasting uptake of particles from spin-coated lms by cells, as it is advantageous in potential later applications, can only be achieved by further time consuming surface modications.…”

Section: Model Drug Loading and Releasementioning

confidence: 99%

“…19 Wiltschka et al reported on coating of pre-formed mesoporous silica nanoparticles with a diameter of about 400 nm on glass slides by spin-coating. 20 By a further functionalization of glass slides with hyaluronic acid it was possible to covalently link a sub-monolayer of particles to the substrate, leading to a delayed uptake of particles by cells over several days. 21 This allowed a potentially delayed particle-mediated release of small hydrophobic drugs within cells.…”

Section: Introductionmentioning

confidence: 99%

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Cell adherence and drug delivery from particle based mesoporous silica films

Björk

Baumann

Hausladen³

et al. 2019

RSC Adv.

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Spatially and temporally controlled drug delivery is important for implant and tissue engineering applications, as the efficacy and bioavailability of the drug can be enhanced, and can also allow for drugging stem cells at different stages of development. Long-term drug delivery over weeks to months is however difficult to achieve, and coating of 3D surfaces or creating patterned surfaces is a challenge using coating techniques like spin-and dip-coating. In this study, mesoporous films consisting of SBA-15 particles grown onto silicon wafers using wet processing were evaluated as a scaffold for drug delivery.Films with various particle sizes (100-900 nm) and hence thicknesses were grown onto trichloro(octadecyl)silane-functionalized silicon wafers using a direct growth method. Precise patterning of the areas for film growth could be obtained by local removal of the OTS functionalization through laser ablation. The films were incubated with the drug model 3,3 0 -dioctadecyloxacarbocyanine perchlorate (DiO), and murine myoblast cells (C2C12 cells) were seeded onto films with different particle sizes. Confocal laser scanning microscopy (CLSM) was used to study the cell growth, and a vinculinmediated adherence of C2C12 cells on all films was verified. The successful loading of DiO into the films was confirmed by UV-vis and CLSM. It was observed that the drugs did not desorb from the particles during 24 hours in cell culture. During adherent growth on the films for 4 h, small amounts of DiO and separate particles were observed inside single cells. After 24 h, a larger number of particles and a strong DiO signal were recorded in the cells, indicating a particle mediated drug uptake. The vast majority of the DiO-loaded particles remained attached to the substrate also after 24 h of incubation, making the films attractive as longer-term reservoirs for drugs on e.g. medical implants. † Electronic supplementary information (ESI) available: Details on substrate preparation, TEM micrographs of the materials, a CLSM micrograph of a lm aer 24 h of cell cultivation, and a CLSM micrograph of C2C12 cells on a lm. See

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Section: Model Drug Loading and Releasementioning

confidence: 99%

Section: Model Drug Loading and Releasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cell adherence and drug delivery from particle based mesoporous silica films

Björk

Baumann

Hausladen³

et al. 2019

RSC Adv.

Self Cite

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“…29 This lack in the uptake of free model drug at dye concentration levels present in the supernatant after release indicates that a cellular uptake of drugs is favored by intracellular release of drugs from loaded particles from the film surface under the studied conditions, fully in line with previously reported results obtained for spin-coated particulate films. 16 The possibility of particle-mediated transport of drugs was investigated by further cell experiments. Therefore, cells were seeded on the surface of DiG_0.00 films loaded with the model drug DiO and the uptake of drug and particles was investigated by CLSM.…”

Section: Drug Loading and Releasementioning

confidence: 99%

“…15 Wiltschka et al reported on coating of pre-formed mesoporous silica nanoparticles with a diameter of about 400 nm on glass slides by spin-coating. 16 By a further functionalization of glass slides with hyaluronic acid it was possible to covalently link a sub-monolayer of particles to the substrate, leading to a delayed uptake of particles by cells over several days. 17 This allowed a potentially delayed particle-mediated release of small hydrophobic drugs within cells.…”

Section: Introductionmentioning

confidence: 99%

Cell Adherence and Drug Delivery from Particle Based Mesoporous Silica Films

Björk

Baumann²,

Hausladen³

et al. 2019

Preprint

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Spatially and temporally controlled drug delivery is important for implant and tissue engineering applications, as the efficacy and bioavailability of the drug can be enhanced, and can also allow for drugging stem cells at different stages of development. Long-term drug delivery over weeks to months is however difficult to achieve, and coating of 3D surfaces or creating patterned surfaces is a challenge using coating techniques like spin- and dip-coating. In this study, mesoporous films consisting of SBA-15 particles grown onto silicon wafers using wet processing were evaluated as a scaffold for drug delivery. Films with various particle sizes (100 – 900 nm) and hence thicknesses were grown onto OTS-functionalized silicon wafers using a direct growth method. Precise patterning of the areas for film growth could be obtained by local removal of the OTS functionalization through laser ablation. The films were incubated with the model drug DiO, and murine myoblast cells (C2C12 cells) were seeded onto films with different particle sizes. Confocal laser scanning microscopy (CLSM) was used to study the cell growth, and a vinculin-mediated adherence of C2C12 cells on all films was verified. The successful loading of DiO into the films was confirmed by UV-vis and CLSM. It was observed that the drugs did not desorb from the particles during 24 hours in cell culture. During adherent growth on the films for 4 h, small amounts of DiO and separate particles were observed inside single cells. After 24 h, a larger number of particles and a strong DiO signal were recorded in the cells, indicating a particle mediated drug uptake. A substantial amount of DiO loaded particles were however attached on the substrate after 24 making the films attractive as a long-term reservoir for drugs on e.g. medical implants.<br>

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Universität Ulm

2017

Geschichte Der Anorganischen Chemie

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Preparation, characterization, and preliminary biocompatibility evaluation of particulate spin-coated mesoporous silica films

Cited by 19 publications

References 37 publications

Cell adherence and drug delivery from particle based mesoporous silica films

Cell adherence and drug delivery from particle based mesoporous silica films

Cell Adherence and Drug Delivery from Particle Based Mesoporous Silica Films

Universität Ulm

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