Preparation, Characterization and Manipulation of Conjugates between Gold Nanoparticles and DNA

Eidelshtein, Gennady; Fattal, Moran; Avishai, Gavriel; Kempinski, Benjamin; Giannini, Clelia; Kotlyar, Alexander

doi:10.3390/nano6090167

Cited by 4 publications

(2 citation statements)

References 25 publications

(33 reference statements)

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“…The difference in mobility between AuNPs loaded with short DNA strands is observed generally in 1.5−3% gels. 31,45,46 AuNPs with adsorbed T 6 have close ζ-potential values with intact citrate-coated AuNPs (Figure 3A), suggesting that a similar quantity of negative charges is displaced by binding oligonucleotide. Longer DNA chains significantly increase the negative ζ-potential of AuNPs.…”

Section: ■ Results and Discussionmentioning

confidence: 88%

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Fast and Strong Adsorption of Native Oligonucleotides on Citrate-Coated Gold Nanoparticles

et al. 2017

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The adsorption of oligonucleotides on citrate-coated gold nanoparticles (AuNPs) is studied under conditions "right after the synthesis", i.e., in a weak citrate solution at a pH value close to neutral (5.8 ± 0.2). We found that short-term elevation of reaction temperature under these conditions provides fast and strong adsorption of oligonucleotides on the surface of AuNPs. The affinity of oligonucleotides to AuNPs depends on the length of the oligonucleotide and its nucleotide composition. The shortest oligonucleotide in this study, T, is the most affine, having the equilibrium binding constant K = 0.10 ± 0.04 nM and the highest surface density-up to 200 molecules per one particle. Olygothymidylates are at least as affine to AuNPs as oligoadenylates, while oligocytidilates show the lowest affinity. We also studied the interaction of resulting DNA/AuNPs with a series of low- and high-molecular thiols, which provide a variety of operations with adsorbed oligonucleotides: displacement (complete or partial) and encapsulation in a secondary shell. These experiments imitate someway the conditions in a living cell or serum, and show that DNA/AuNPs obtained by this method can be applied in a number of bionanotechnological applications, including delivery of nucleic acid therapeutics and theranostics.

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Section: ■ Results and Discussionmentioning

confidence: 88%

“…This could be explained by large pore size, which is characteristic for 0.8% agarose gel. The difference in mobility between AuNPs loaded with short DNA strands is observed generally in 1.5–3% gels. ,, …”

Section: Resultsmentioning

confidence: 96%