Preparation, characterization, and enhanced antimicrobial activity: quercetin-loaded PLGA nanoparticles against foodborne pathogens

Abstract

The use of quercetin as a bioflavonoid is becoming increasingly common in food industries even though poor water solubility, instability, absorption, and permeability have limited its application. The oil-in-water single-emulsion solvent evaporation method to synthesize highly stable and soluble quercetin-encapsulated nanoparticles (NPs), in which the reaction yield, particle size, and polydispersity of the NPs are varied greatly within the process parameters of the synthesis method, has been optimized. NPs with different initial quercetin amounts were used to determine how the quercetin amount affected nanoparticle properties and antimicrobial efficiency. Listeria monocytogenes, Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus were chosen as model bacteria due to their being foodborne pathogens. The results of antimicrobial activity evaluated by three different methods showed that the antimicrobial activity of both quercetin NPs and free quercetin was effective on gram-positive strains (L. monocytogenes and S. aureus). Additionally, it was detected that Q31 NPs have more effective antimicrobial activity than other synthesized quercetin nanoparticles depending on the amount of substance and release. Furthermore, on the basis of assessing the antibacterial effects by scanning electron microscopy, it was detected that bacteria cells lost their integrity and became pale with the release of cytoplasm and decomposed after treatment with Q31 NPs.

Design of cefaclor monohydrate containing nanoparticles with extended antibacterial effect by Nano-Spray dryer: A Nanoenglobing study

“…In a study as an example, bacterial surface charges of S. aureus and E. coli were reported as -13.1 mV and -2.1, respectively. It was emphasized in this study that the positive ZP formulations designed were more effective [38]. Similarly, the more effective NP-2 in our study can be explained by the high positive ZP of it.…”

Cytotoxic and mutagenic potential of juglone: a comparison of free and nano-encapsulated form

“…Considering its high antimicrobial effect against infections, juglone bears a promise in this respect. It has already been evidenced for herbicidal ( 9 ), antibacterial ( 10 , 11 , 12 , 13 ), antiviral ( 14 ), antifungal ( 15 , 16 ), antioxidant ( 13 ), antiparasitic ( 17 ), and anticancer ( 18 , 19 , 20 ) properties. However, its hydrophobic structure and high toxicity limit its application in biological systems ( 2 , 4 ).…”

A New Approach To The Treatment of Leıshmaniasis: Quercetin-Loaded Polycaprolactone Nanoparticles

“…In the study by Arasoglu et al, this technique was shown to be more successful than nano-precipitation and salting out techniques used in the encapsulation of quercetin by PLGA nanoparticles. In the same study, it was found that the nanoparticles synthesized using single emulsion solvent evaporation have higher reaction yield and have a more significant antibacterial activity compared to the nanoparticles synthesized by other techniques (27). Size analysis and other characterization tests of the nanoparticles we prepared using oil-in-water single emulsion solvent evaporation technique were performed using SEM and zetasizer devices.…”

“…While quercetin and quercetin-loaded PLGA nanoparticles showed nearly similar levels of antibacterial activity on Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus, quercetin-loaded PLGA nanoparticles showed higher level of antibacterial activity on Listeria monocytogenes. The MIC value of quercetin-loaded PLGA nanoparticles on these bacteria was 100 µg/mL whereas the MIC value of quercetin alone was 200 µg/mL (27). In a similar study from 2016, Sun et al investigated the in vitro and in vivo antibacterial effects of quercetin-loaded PLGA nanoparticles on E. coli and Micrococcus tetragenus bacteria.…”