Preparation, Characterization, and Dissolution Rate In Vitro Evaluation of Total Panax Notoginsenoside Nanoparticles, Typical Multicomponent Extracts from Traditional Chinese Medicine, Using Supercritical Antisolvent Process

Abstract

TotalPanaxnotoginsenosides nanoparticles, typical multicomponent extracts from traditional Chinese medicine, were prepared with a supercritical antisolvent (SAS) process using ethanol as solvent and carbon dioxide as antisolvent. The optimum conditions were determined to be as follows: TPNS solution concentration of 2.5 mg/mL, TPNS solution flow rate of 6.6 mL/min, precipitation temperature of 40°C, and precipitation pressure of 20 MPa. Under the optimum conditions, TPNS nanoparticles with a MPS of 141.5 ± 18.2 nm and total saponins amounts (TSA) of 78.9% were obtained. The TPNS nanoparticles obtained were characterized by scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimeters (DSC), and high performance liquid chromatography (HPLC). The results showed that the chemical and crystal structure of the obtained TPNS nanoparticles has not changed. Dissolutionin vitrostudies showed that the solubility and dissolution rate of notoginsenosides R1 and ginsenoside Rb1 in TPNS nanoparticles are higher than these in raw TPNS, with no obvious difference in Rg1. These results suggest that TPNS nanoparticles can be helpful to the improvement of its bioavailability for the treatment of cardiovascular diseases.

Recent trends of nano bioactive compounds from ginseng for its possible preventive role in chronic disease models

“…Novel green techniques can thus be used for a supercritical antisolvent process with total panax notoginsenoside nano particle synthesized with a size ranging from 141.5 nm with a higher bioavailability of the compounds during in vitro studies. 61 In a manner similar to the sizing, some delivery carrier techniques have also improved the anticancer activity. Recently, 20(S)-ginsenoside Rg 3 , an active ginseng compound, was constructed with magnetic particles with an easy delivery system of human serum albumin to form nanospheres using the desolvation-crosslinking technique, and when tested with HeLa cervical cancer cells, these showed a higher anti-cancer activity.…”

Preparation of Cefquinome Nanoparticles by Using the Supercritical Antisolvent Process

“…Because of the advantages of low operating temperature, no solvent residue, and controllable particle size, supercritical fluid method attracted more and more attention of researchers worldwide. The processes of preparing nanodrug by supercritical fluid include two categories: rapid expansion of supercritical solution (RESS) and supercritical antisolvent (SAS) [10][11][12]. Because most drugs cannot be dissolved in the supercritical fluid, RESS process is restricted and SAS process has applied more and more in nanodrug preparations.…”