Preparation, characterization and anti-ageing activity of Gastrodia elata blume polysaccharide

Abstract

Gastrodia elata Blume polysaccharide (GEP) was extracted and then chemically characterised. Its antioxidant activity was evaluated in vitro and in vivo. The results of the in vitro investigation show that GEP consists of glucose with molecular weight of 875 185 Da and exhibits high hydroxyl radical scavenging, as well as 2,2-diphenyl-1picrylhydrazyl activity and reducing capacity. For antioxidant activity in vivo, D-galactose-induced-aged mice were orally administered with three different doses of GEP over a period of 6 weeks. The administration of GEP dosedependently increased the body weight gain rates, liver and brain indices, superoxide dismutase and glutathione peroxidase activities, and malondialdehyde levels in the sera and brains of ageing mice. These results suggest that GEP exhibits high antioxidant activity and can retard human ageing associated with free radicals.

Antioxidant Activities of Natural Polysaccharides and Their Derivatives for Biomedical and Medicinal Applications

“…One study extracted GP by the hot water extraction method, and chemical characterization by UV spectrometer and HPLC, then detected its antioxidant activity in vitro and in vivo. The results showed that GP could effectively remove hydroxyl radicals and has a substantial reduction ability [ 66 ]. Oral GP can increase the activities of SOD and GSH-Px in aging mice induced by D-galactose.…”

Gastrodia elata Blume Polysaccharides: A Review of Their Acquisition, Analysis, Modification, and Pharmacological Activities

“…The merits of these methods are their compatibility with polysaccharides. Due to the fact the Sevag method is much milder than the others, G. elata aqueous extract is typically deproteinized using the Sevag method [23,41,54]. The enzyme method is another effective and alternative to the common methods to eliminate proteins.…”

“…Using a refractive index detector, Chen et al reported that G. elata RGP-1a is composed of fructose and glucose in a ratio of 1:10.68, and G. elata RGP-1b is only composed of glucose [24]. Using a carbohydrate column and a pulsed amperometric detector, Zhu et al reported that the G. elata polysaccharide GEP is composed of glucose [54]. Through the use of ion chromatography to analyze the monosaccharide composition of G. elata polysaccharides, Li et al reported that the G. elata polysaccharide is composed of rhamnose, galactose, glucose, xylose, and mannose [65].…”

“…A series of glucans with different molecular weights is used to establish the standard curve and determine the molecular weights of polysaccharides. All the molecular weights of G. elata polysaccharides, such as the polysaccharides WGEW, AGEW [10], WTMA [27], PGEB-3H [25], RGP-1a, RGP-1b [23], GPs [13], PGE [28], and GEP [54], are determined according to this method.…”

Optimal Extraction Study of Gastrodin-Type Components from Gastrodia Elata Tubers by Response Surface Design with Integrated Phytochemical and Bioactivity Evaluation

“…The protection of this phytomedicine on human umbilical vein endothelial cells (HUVEC) involved in these bioactivities [28,29]. On the other hand, with many polyphenols including the gastrodin-type components mentioned above, GET exerted predominant antioxidant activity, which also played a crucial role in its efficacies and was related to many pharmacological bioactivities, such as protection on liver and kidney jury, antiaging, reducing cerebral ischemia/reperfusion injury, antihypertension, neuroprotection [30,31,32,33,34,35,36]. Hence, HUVEC protective and antioxidant properties could also be utilized to reflect GET quality.…”