Optimal Extraction Study of Gastrodin-Type Components from Gastrodia Elata Tubers by Response Surface Design with Integrated Phytochemical and Bioactivity Evaluation

Abstract

Gastrodia elata tuber (GET) is a popular traditional Chinese medicines (TCMs). In this study, response surface methodology (RSM) with a Box–Behnken design (BBD) was performed to optimize the extraction parameters of gastrodin-type components (gastrodin, gastrodigenin, parishin A, parishin B, parishin C and parishin E). Different from the conventional studies that merely focused on the contents of phytochemical, we gave consideration to both quantitative analysis of the above six components by HPLC and representative bioactivities of GET, including antioxidation and protection of human umbilical vein endothelial cells (HUVEC). Four independent variables (ethanol concentration, liquid-material ratio, soaking time and extraction time) were investigated with the integrated evaluation index of phytochemical contents. With the validation experiments, the optimal extraction parameters were as follows: ethanol concentration of 41%, liquid–solid ratio of 28.58 mL/g, soaking time of 23.91 h and extraction time of 46.60 min. Under the optimum conditions, the actual standardized comprehensive score was 1.8134 ± 0.0110, which was in accordance with the predicted score of 1.8100. This firstly established method was proved to be feasible and reliable to optimize the extraction parameters of the bioactive components from GET. Furthermore, it provides some reference for the quality control and extraction optimization of TCMs.

Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

“…Gastrodia elata (G. elata) Blume is a traditional medicinal herb that has been used in oriental countries, for centuries, to treat general paralysis, headaches, dizziness, rheumatism, convulsion, and epilepsy [1,2]. Modern pharmacological studies have demonstrated that the extracts of G. elata tuber and some compounds that originate from it, possesses wide-reaching biological activities, including anti-tumor, anti-virus, memory-improving, anti-oxidation, and anti-aging actions [3,4,5]. Nowadays, it is also widely used as a sub-material in food and Chinese Patent Medicines (CPM) [6], and this herbal medicine is also listed as one of the functional foods approved by the Ministry of Health in China [7,8].…”

Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume

“…Lin et al [18] found that PC significantly ameliorated long-term potentiation (LTP) injury induced by amyloid β1-42 (Aβ1-42). In addition, it was found that PA had anti-aging effects [19], while parishin B (PB) had anti-asthma effects [20]. These findings suggested that parishins played a very important role in pharmacodynamics of RGE and their contents were much higher than that of GAS [21,22].…”

Metabolic engineering of Saccharomyces cerevisiae for high-level production of gastrodin from glucose

“…In addition to its neuroprotective effects [ 3 ], gastrodin has been proven to show memory improvement [ 4 ], anti-inflammation, and antioxidant effects [ 5 , 6 ]. To date, gastrodin is produced mainly by chemical synthesis or direct extraction from Tianma, although different processing methods have also been explored and applied [ 7 , 8 ]. However, total chemical synthesis is a complicated process and leads to severe environmental pollution due to the use of toxic phenols, phosphate and bromide.…”