Preparation and structural characterization of peanut peptide–zinc chelate

Abstract

Peanut protein isolate was hydrolyzed by trypsin. The resulting peanut peptide fractions (PPF) were then divided into three molecular weight components by ultrafiltration (PPF1, MW >5 kDa; PPF2, 3 kDa < MW <5 kDa; PPF3, MW <3 kDa; and untreated PPF). Experiments showed that PPF3 had the best capacity for chelation with zinc. PPF3 and the PPF3-Zn chelate were characterized by Ultraviolet spectrophotometry, Fourier-transform infrared spectroscopy, X-ray diffraction, Scanning electron microscopy, Energy disperse spectroscopy, Fluorescence spectroscopy, and Thermogravimetric analysis. The results showed that the apparent characteristics and internal structures of PPF3 were changed by chelation of Zn 2+ . PPF3 contained sufficient metal-binding sites, with -COOH, -OH, and -NH 2 potentially participating in coordination with Zn 2+ to form the PPF3-Zn chelate. Furthermore, the results suggested that peanut peptides might chelate with zinc to act as an emerging carrier for zinc supplements.Preparación y caracterización estructural del quelato de péptido de cacahuate y zinc RESUMEN Para fines del presente estudio, el aislado de proteína de cacahuate fue hidrolizado empleando tripsina. Empleando ultrafiltración, se dividieron las fracciones peptídicas del cacahuate (PPF) en tres componentes de peso molecular. (PPF1, MW >5 kDa; PPF2, 3 kDa < MW <5 kDa; PPF3, MW <3 kDa; y PPF no tratado). Los experimentos constataron que el PPF3 tiene la mejor capacidad de quelación con el zinc. El PPF3 y el quelato de PPF3-Zn fueron caracterizados utilizando espectrofotometría ultravioleta, espectroscopia infrarroja por transformada de Fourier, difracción de rayos X, microscopía electrónica de barrido, espectroscopia de energía dispersa, espectroscopia de fluorescencia y análisis termogravimétrico. Los resultados dieron cuenta de que las características aparentes y las estructuras internas del PPF3 se modificaron a partir de la quelación del Zn 2+ . El PPF3 mostró suficientes sitios de unión de metales, con -COOH, -OH y -NH 2, participando potencialmente para formar el quelato PPF3-Zn en coordinación con el Zn 2+ . Además, los resultados establecieron que los péptidos del cacahuate podrían quelatarse con el zinc para actuar como portadores emergentes de suplementos de zinc.

Preparation, structure characterization, and stability analysis of peptide–calcium complex derived from porcine nasal cartilage type II collagen

“…7, the temperature required for PNCPs‐Ca to reach its minimum mass was higher than that required for PNCPs. This was because of the increased intermolecular force between the peptide and calcium ions, which increases the temperature required for decomposition and results in higher molecular energy and greater thermal stability 35 …”

“…This was because of the increased intermolecular force between the peptide and calcium ions, which increases the temperature required for decomposition and results in higher molecular energy and greater thermal stability. 35…”

Cellular responses of histatin-derived peptides immobilized titanium surface using a tresyl chloride-activated method

“…In addition, FTIR spectra showed the presence of the amino groups on the Ti surface after coated with peptides, the spectra appearing at 680 cm −1 were assigned to the wagging mode of N-H (primary and secondary amines) and the bending mode of N-H (primary amines) also was observed at 1,590 cm − 1 28,29) . Moreover, Chen Li et al combined EDS and FTIR analyses to confirm the peptide structures 42) .…”

A critical review of a typical research system for food‐derived metal‐chelating peptides: Production, characterization, identification, digestion, and absorption

“…Protein content of coconut cake was high (15–20 g/100 g), the yield was high, and the nontoxic peptides extracted and identified from coconut cakes appropriately complexed with zinc ions, which was 30.80 mg/g (Zheng et al., 2023). Similarly, peanut protein extracted from peanut meal, a byproduct of oil extraction, is a high‐quality MCP source, and the zinc ion chelation rate of peanut peptide (<3 kDa) is 53.01% (Li et al., 2020). In summary, compared with terrestrial sources, plant‐based byproducts are still rich in proteins, and research and industrial applications of MCPs should focus on improving the recycling of these by‐products.…”