Preparation and Recellularization of Tissue Engineered Bioscaffold for Heart Valve Replacement

Fujisato, Toshiya; Minatoya, Kenji; Yamazaki, Sachiko; Yin, Meng; Niwaya, Kazuo; Kishida, Akio; Nakatani, Takeshi; Kitamura, Soichiro

doi:10.1007/4-431-27378-6_7

Cited by 19 publications

(13 citation statements)

References 10 publications

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“…Cell components in the amnion were removed by high hydrostatic pressure treatment as reported previously. 27,28 The resulting decellularized amnion was stored in the Dulbecco's modified Eagle's medium containing 50% glycerol (Invitrogen) at -80°C until use.…”

Section: Amniotic Membrane Preparationmentioning

confidence: 99%

Periodontal Regeneration Using Periodontal Ligament Stem Cell-Transferred Amnion

Iwasaki

Komaki²,

Yokoyama³

et al. 2013

Tissue Engineering Part A

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Periodontal disease is characterized by the destruction of tooth supporting tissues. Regeneration of periodontal tissues using ex vivo expanded cells has been introduced and studied, although appropriate methodology has not yet been established. We developed a novel cell transplant method for periodontal regeneration using periodontal ligament stem cell (PDLSC)-transferred amniotic membrane (PDLSC-amnion). The aim of this study was to investigate the regenerative potential of PDLSC-amnion in a rat periodontal defect model. Cultured PDLSCs were transferred onto amniotic membranes using a glass substrate treated with polyethylene glycol and photolithography. The properties of PDLSCs were investigated by flow cytometry and in vitro differentiation. PDLSC-amnion was transplanted into surgically created periodontal defects in rat maxillary molars. Periodontal regeneration was evaluated by microcomputed tomography (micro-CT) and histological analysis. PDLSCs showed mesenchymal stem cell-like characteristics such as cell surface marker expression (CD90, CD44, CD73, CD105, CD146, and STRO-1) and trilineage differentiation ability (i.e., into osteoblasts, adipocytes, and chondrocytes). PDLSC-amnion exhibited a single layer of PDLSCs on the amniotic membrane and stability of the sheet even with movement and deformation caused by surgical instruments. We observed that the PDLSC-amnion enhanced periodontal tissue regeneration as determined by micro-CT and histology by 4 weeks after transplantation. These data suggest that PDLSC-amnion has therapeutic potential as a novel cell-based regenerative periodontal therapy.

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Section: Amniotic Membrane Preparationmentioning

confidence: 99%

Periodontal Regeneration Using Periodontal Ligament Stem Cell-Transferred Amnion

Iwasaki

Komaki²,

Yokoyama³

et al. 2013

Tissue Engineering Part A

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“…Human placentas were obtained from a Caesarean section of women who was seronegative for human immunodeficiency virus, human hepatitis types B and C, and syphilis. Decellularization of amniotic membrane was conducted as previously described (Fujisato, et al, 2005).…”

Section: Preparation Of Amniotic Membranementioning

confidence: 99%

Therapeutic Angiogenesis by Implantation of a Capillary Structure Constituted of Human Adipose Tissue Microvascular Endothelial Cells

Yoshida

Komaki

Hattori

et al. 2010

ATVB

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Objective-We previously reported a novel technology for the engineering of a capillary network using an optical lithographic technique. To apply this technology to the therapy of ischemic diseases, we tested human omental microvascular endothelial cells (HOMECs) as an autologous cell source and decellularized human amniotic membranes (DC-AMs) as a pathogen-free and low immunogenic transplantation scaffold. Methods and Results-Human umbilical vein endothelial cells were aligned on a patterned glass substrate and formed a capillary structure when transferred onto an amniotic membrane (AM). In contrast, HOMECs were scattered and did not form a capillary structure on AMs. Treatment of HOMECs with sphingosine 1-phosphate (S1P) inhibited HOMEC migration and enabled HOMEC formation of a capillary structure on AMs. Using quantitative RT-PCR and Western blot analyses, we demonstrated that the main S1P receptor in HOMECs is S1P 2 , which is lacking in human umbilical vein endothelial cells, and that inhibition of cell migration by S1P is mediated through an S1P 2 -Rho-Rho-associated kinase signaling pathway. Implantation of capillaries engineered on DC-AMs into a hindlimb ischemic nude mouse model significantly increased blood perfusion compared with controls. Conclusion-A capillary network consisting of HOMECs on DC-AMs can be engineered ex vivo using printing technology and S1P treatment. This method for regeneration of a capillary network may have therapeutic potential for ischemic diseases. Key Words: angiogenesis Ⅲ angioplasty Ⅲ endothelium Ⅲ ischemia Ⅲ peripheral arterial disease Ⅲ vascular biology I schemic diseases, including peripheral arterial diseases (PADs), myocardial infarction, and cerebral infarction, threaten our health and our lives. When medication becomes ineffective, patients need to undergo percutaneous transluminal angioplasty or artery bypass grafting. Recently, therapeutic angiogenesis has been developed for no-option patients. The target of percutaneous transluminal angioplasty and artery bypass grafting is blood vessels with a diameter larger than 1 mm, whereas that of angiogenic therapy is capillaries and microvessels with a diameter on the micrometer order. See accompanying article on page 1277Therapeutic angiogenesis consists of 2 strategies: (1) growth factor therapy and (2) cell therapy. It has been applied to ischemic diseases, including PADs, such as arteriosclerosis obliterans and Burger's disease, as well as myocardial infarction and cerebral infarction. 1,2 The aim of growth factor therapy is to induce angiogenesis by gene therapy or by protein administration of angiogenic factors. Although early nonblinded and uncontrolled studies showed promising outcomes, some later double-blinded, randomized, and controlled trials have been less effective than anticipated. 3 More recently, cell therapy has been developed as a second angiogenic therapy. Initially, peripheral blood-derived or bone marrow-derived mononuclear cells (MNCs) were transplanted as endothelial progenitor cells, which should ...

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“…As another candidate for a new decellularization treatment, we have reported our work on the high-hydrostatic pressure (HHP) method [14,15]. The unique characteristics of the HHP method are the destruction of cell membranes, uniform treatment, and short treatment time.…”

Section: Introductionmentioning

confidence: 99%

The use of high-hydrostatic pressure treatment to decellularize blood vessels

Funamoto¹,

Nam²,

Kimura³

et al. 2010

Biomaterials

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Preparation and Recellularization of Tissue Engineered Bioscaffold for Heart Valve Replacement

Cited by 19 publications

References 10 publications

Periodontal Regeneration Using Periodontal Ligament Stem Cell-Transferred Amnion

Periodontal Regeneration Using Periodontal Ligament Stem Cell-Transferred Amnion

Therapeutic Angiogenesis by Implantation of a Capillary Structure Constituted of Human Adipose Tissue Microvascular Endothelial Cells

The use of high-hydrostatic pressure treatment to decellularize blood vessels

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