Preparation and Optimization of a Biomimetic Triple-Layered Vascular Scaffold Based on Coaxial Electrospinning

Hu, Qingxi; Wu, Chuang; Zhang, Haiguang

doi:10.1007/s12010-019-03147-2

Cited by 13 publications

(5 citation statements)

References 55 publications

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“…Our observations are consistent with previous reports, which have shown coaxial electrospinning is an efficient strategy for surface modification of nanofibrous scaffolds made from synthetic polymers like PCL, PLGA, and polyvinyl alcohol (PVA) ( Kim and Cho, 2016 ). While coaxial electrospinning has been more commonly used for controlled drug/biomolecule release ( Zhang et al, 2006 ; Ji et al, 2010 ; Sun et al, 2019 ), it has also been used to coat synthetic polymer-based nanofibers with a natural polymer (like gelatin, alginate, and collagen) to make them more biomimetic ( Zhang et al, 2007 ; Hu et al, 2020 ). The use of coaxial nanofibers with a PCL inner core and gelatin outer shell has previously been used for wound healing ( Blackstone et al, 2014 ) and vascular ( Coimbra et al, 2017 ) and bone ( Alissa Alam et al, 2020 ) tissue engineering applications.…”

Section: Discussionmentioning

confidence: 99%

Scalable Biomimetic Coaxial Aligned Nanofiber Cardiac Patch: A Potential Model for “Clinical Trials in a Dish”

Kumar

Sridharan

Palaniappan

et al. 2020

Front. Bioeng. Biotechnol.

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Recent advances in cardiac tissue engineering have shown that human inducedpluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) cultured in a threedimensional (3D) micro-environment exhibit superior physiological characteristics compared with their two-dimensional (2D) counterparts. These 3D cultured hiPSC-CMs have been used for drug testing as well as cardiac repair applications. However, the fabrication of a cardiac scaffold with optimal biomechanical properties and high biocompatibility remains a challenge. In our study, we fabricated an aligned polycaprolactone (PCL)-Gelatin coaxial nanofiber patch using electrospinning. The structural, chemical, and mechanical properties of the patch were assessed by scanning electron microscopy (SEM), immunocytochemistry (ICC), Fourier-transform infrared spectroscopy (FTIR)-spectroscopy, and tensile testing. hiPSC-CMs were cultured on the aligned coaxial patch for 2 weeks and their viability [lactate dehydrogenase (LDH assay)], morphology (SEM, ICC), and functionality [calcium cycling, multielectrode array (MEA)] were assessed. Furthermore, particle image velocimetry (PIV) and MEA were used to evaluate the cardiotoxicity and physiological functionality of the cells in response to cardiac drugs. Nanofibers patches were comprised of highly aligned core-shell fibers with an average diameter of 578 ± 184 nm. Acellular coaxial patches were significantly stiffer than gelatin alone with an ultimate tensile strength of 0.780 ± 0.098 MPa, but exhibited gelatin-like biocompatibility. Furthermore, hiPSC-CMs cultured on the surface of these aligned coaxial patches (3D cultures) were elongated and rod-shaped with well-organized sarcomeres, as observed by the expression of cardiac troponin-T and α-sarcomeric actinin. Additionally, hiPSC-CMs cultured on these coaxial patches formed a functional syncytium evidenced by the expression of connexin-43 (Cx-43) and

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Section: Discussionmentioning

confidence: 99%

Scalable Biomimetic Coaxial Aligned Nanofiber Cardiac Patch: A Potential Model for “Clinical Trials in a Dish”

Kumar

Sridharan

Palaniappan

et al. 2020

Front. Bioeng. Biotechnol.

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“…Subsequently, we removed the ethanol-saturated scaffold out and weighed the pycnometer again ( W 3 ). Based on these data, we referred to previous studies − and calculated by the following equations where V S means the volume of the scaffold, V P means the volume of the scaffold, and ρ represents the density of absolute ethanol.…”

Section: Materials and Methodsmentioning

confidence: 99%

3D Polycaprolactone/Gelatin-Oriented Electrospun Scaffolds Promote Periodontal Regeneration

Zhou

Zheng

et al. 2022

ACS Appl. Mater. Interfaces

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Periodontitis is a worldwide chronic inflammatory disease, where surgical treatment still shows an uncertain prognosis. To break through the dilemma of periodontal treatment, we fabricated a three-dimensional (3D) multilayered scaffold by stacking and fixing electrospun polycaprolactone/gelatin (PCL/Gel) fibrous membranes. The biomaterial displayed good hydrophilic and mechanical properties. Besides, we found human periodontal ligament stem cell (hPDLSC) adhesion and proliferation on it. The following scanning electron microscopy (SEM) and cytoskeleton staining results proved the guiding function of fibers to hPDLSCs. Then, we further analyzed periodontal regeneration-related proteins and mRNA expression between groups. In vivo results in a rat acute periodontal defect model confirmed that the topographic cues of materials could directly guide cellular orientation and might provide the prerequisite for further differentiation. In the aligned scaffold group, besides new bone regeneration, we also observed that angular concentrated fiber regeneration in the root surface of the defect is similar to the normal periodontal tissue. To sum up, we have constructed electrospun membrane-based 3D biological scaffolds, which provided a new treatment strategy for patients undergoing periodontal surgery.

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“…In addition, the exchange of growth factors could also be achieved by BMVs [30,31]. To date, a number of engineering technologies such as micro-modeling [32], coaxial electrospinning [33], 3D printing [34][35][36] and microfluidic technology [37][38][39] have been used for building BMVs in vitro [40][41][42]. Among them, microfluidic technology (or microfluidics), possessing unique advantages including low cost, simple operation and flexibility in controlling microscale fluid, has been widely used in the preparation of microscale constructs [43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Endothelialized microvessels fabricated by microfluidics facilitate osteogenic differentiation and promote bone repair

Wang

et al. 2022

Acta Biomaterialia

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Preparation and Optimization of a Biomimetic Triple-Layered Vascular Scaffold Based on Coaxial Electrospinning

Cited by 13 publications

References 55 publications

Scalable Biomimetic Coaxial Aligned Nanofiber Cardiac Patch: A Potential Model for “Clinical Trials in a Dish”

Scalable Biomimetic Coaxial Aligned Nanofiber Cardiac Patch: A Potential Model for “Clinical Trials in a Dish”

3D Polycaprolactone/Gelatin-Oriented Electrospun Scaffolds Promote Periodontal Regeneration

Endothelialized microvessels fabricated by microfluidics facilitate osteogenic differentiation and promote bone repair

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