Endothelialized microvessels fabricated by microfluidics facilitate osteogenic differentiation and promote bone repair

Wang, Jiayuan; Wang, Huan; Wang, Yong; Liu, Zhao; Li, Zexi; Chen, Qixin; Meng, Qingchen; Shu, Wenmiao; Wu, Junxi; Chu, Xiao; Han, Fengxuan; Li, Bin

doi:10.1016/j.actbio.2022.01.055

Cited by 24 publications

(39 citation statements)

References 75 publications

(77 reference statements)

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“…BMP-2 released by vascular endothelial cells is an essential regulator of osteogenic differentiation. 74 Moreover, VEGF secreted by BMSCs plays a vital role in promoting the migration and differentiation of endothelial cells and vascularization. BMSCs and endothelial cells communicate dynamically through synergy to promote new blood vessel formation and bone regeneration.…”

Section: Discussionmentioning

confidence: 99%

Synergistic effects of nanoattapulgite and hydroxyapatite on vascularization and bone formation in a rabbit tibia bone defect model

et al. 2022

Biomater. Sci.

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Section: Discussionmentioning

confidence: 99%

Synergistic effects of nanoattapulgite and hydroxyapatite on vascularization and bone formation in a rabbit tibia bone defect model

et al. 2022

Biomater. Sci.

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“…This can be achieved via either encapsulation of cells with osteogenic and chondrogenic properties into biocompatible and biodegradable hydrogel capsules, fibers, ,,,, or other types of structures of desired structural/mechanical properties ,, or via fabrication of microfluidics-based porous hydrogel materials of required morphology (porosity/pore size) aiming to provide a permissive environment for cell proliferation and growth. ,, The majority of the approaches is based on the use of mesenchymal stem cells and bone marrow stem cells due to their high accessibility and well-established differentiation protocols. To support bone regeneration, osteogenic cells are often coseeded with endothelial cells, providing local vascular network aiming to deliver oxygen and nourishment to the engrafted bone tissue, thus enhancing cell survival. ,,, Cartilage, unlike bones, is avascular.…”

Section: Applications Of Topological Microgels In Tissue Engineeringmentioning

confidence: 99%

“…The diversity of the structure and function of blood vessels requires a broad range of microfluidic systems able to address topological differences between arteries, veins, and capillaries. Due to the high structural linearity of the blood vessels in vivo, the majority of the microfluidic-based approaches aiming to recapitulate vascular structures is based on generation of microfibers of various topology and cellular composition ,,,,,,,,,,, and 3D hydrogel scaffolds with bicontinuous topologies or channel-like architectures. ,,, The majority of the models relies on the use of human umbilical vein endothelial cells (HUVECs), ,,,,,,,,,,,, which are easily accessible and can be propagated in culture for relatively long time. Hence, unlike in the case of cardiac and neural microfluidic-based tissue models, in vascularized tissue models, the use of stem cells is not the only viable option and indeed most studies rely on the use of HUVECs.…”

Section: Applications Of Topological Microgels In Tissue Engineeringmentioning

confidence: 99%

Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering

et al. 2022

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Microfluidics has recently emerged as a powerful tool in generation of submillimeter-sized cell aggregates capable of performing tissue-specific functions, so-called microtissues, for applications in drug testing, regenerative medicine, and cell therapies. In this work, we review the most recent advances in the field, with particular focus on the formulation of cell-encapsulating microgels of small "dimensionalities": "0D" (particles), "1D" (fibers), "2D" (sheets), etc., and with nontrivial internal topologies, typically consisting of multiple compartments loaded with different types of cells and/or biopolymers. Such structures, which we refer to as topological hydrogels or topological microgels (examples including core− shell or Janus microbeads and microfibers, hollow or porous microstructures, or granular hydrogels) can be precisely tailored with high reproducibility and throughput by using microfluidics and used to provide controlled "initial conditions" for cell proliferation and maturation into functional tissue-like microstructures. Microfluidic methods of formulation of topological biomaterials have enabled significant progress in engineering of miniature tissues and organs, such as pancreas, liver, muscle, bone, heart, neural tissue, or vasculature, as well as in fabrication of tailored microenvironments for stem-cell expansion and differentiation, or in cancer modeling, including generation of vascularized tumors for personalized drug testing. We review the available microfluidic fabrication methods by exploiting various cross-linking mechanisms and various routes toward compartmentalization and critically discuss the available tissue-specific applications. Finally, we list the remaining challenges such as simplification of the microfluidic workflow for its widespread use in biomedical research, bench-to-bedside transition including production upscaling, further in vivo validation, generation of more precise organ-like models, as well as incorporation of induced pluripotent stem cells as a step toward clinical applications.

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“…In this regard, microfluidic platforms play a crucial role in intensifying the inherent laminar flow and perfusion flow through cells for vasculogenesis and represent a unique system for microvessels perfusion [ 329 , 347 ]. In a recent study, Wang et al developed a microfluidics-based technique for the fabrication of the endothelized biomimetic microvessels (BMVs) by alginate–collagen composites [ 348 ]. The constructed BMVs exhibited a significant perfusion effect that was also able to induce osteogenic differentiation by releasing BMP-2 and PDGF-BB.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Biomedical Applications of Microfluidic Devices: A Review

et al. 2022

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Both passive and active microfluidic chips are used in many biomedical and chemical applications to support fluid mixing, particle manipulations, and signal detection. Passive microfluidic devices are geometry-dependent, and their uses are rather limited. Active microfluidic devices include sensors or detectors that transduce chemical, biological, and physical changes into electrical or optical signals. Also, they are transduction devices that detect biological and chemical changes in biomedical applications, and they are highly versatile microfluidic tools for disease diagnosis and organ modeling. This review provides a comprehensive overview of the significant advances that have been made in the development of microfluidics devices. We will discuss the function of microfluidic devices as micromixers or as sorters of cells and substances (e.g., microfiltration, flow or displacement, and trapping). Microfluidic devices are fabricated using a range of techniques, including molding, etching, three-dimensional printing, and nanofabrication. Their broad utility lies in the detection of diagnostic biomarkers and organ-on-chip approaches that permit disease modeling in cancer, as well as uses in neurological, cardiovascular, hepatic, and pulmonary diseases. Biosensor applications allow for point-of-care testing, using assays based on enzymes, nanozymes, antibodies, or nucleic acids (DNA or RNA). An anticipated development in the field includes the optimization of techniques for the fabrication of microfluidic devices using biocompatible materials. These developments will increase biomedical versatility, reduce diagnostic costs, and accelerate diagnosis time of microfluidics technology.

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Endothelialized microvessels fabricated by microfluidics facilitate osteogenic differentiation and promote bone repair

Cited by 24 publications

References 75 publications

Synergistic effects of nanoattapulgite and hydroxyapatite on vascularization and bone formation in a rabbit tibia bone defect model

Synergistic effects of nanoattapulgite and hydroxyapatite on vascularization and bone formation in a rabbit tibia bone defect model

Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering

Biomedical Applications of Microfluidic Devices: A Review

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