Preparation and Evaluation of Porous Chitosan/Collagen Scaffolds for Periodontal Tissue Engineering

Abstract

The wide array of periodontal tissue engineering applications has demonstrated the need for appropriate biodegradable materials. Porous chitosan/collagen – a natural derivative composite – scaffolds could be a candidate. In this study, differently proportioned porous chitosan/collagen scaffolds were prepared by controlled freezing and lyophylization of corresponding composite solutions. The scaffolds were investigated via aperture, porosity, swelling behavior and cell-mediated contraction testing. The morphology and distribution of human periodontal ligament cells (PDLCs) on these threedimensional scaffolds were investigated by SEM and CLSM. Compared to a single component scaffold, the addition of collagen to chitosan decreased the mean aperture, increased the swelling ability and the addition of chitosan to collagen decreased the contraction. The adherence and growth of PDLCs cultured within the chitosan/collagen scaffolds were better than on single chitosan or collagen scaffolds. It appears that chitosan/collagen composites are promising scaffold materials for periodontal tissue engineering.

Studies on Cross-linking of succinic acid with chitosan/collagen

“…Collagen samples were stirred at 4 °C, whereas, chitosan samples were stirred at ambient temperature. Followed by stirring, the samples were subjected to centrifugation in order to remove any unreacted molecules and a clear solution obtained upon centrifugation at 5,000 RPM for 10 minutes and was poured in Tarson (India) vial of an inner diameter of 4.5 cm and frozen at -4 °C for 2 hours, -20 °C for 12 hours and -80 °C for another 12 hours 19 . The frozen samples were lyophilized for 48 h at a vacuum of 7.5 militorr (1 Pa) and a condenser temperature of -70°C (PENQU CLASSIC PLUS, Lark, India).…”

Synthesis of highly interconnected 3D scaffold from Arothron stellatus skin collagen for tissue engineering application

“…This property of the collagen is mainly due to the hydrophilic group associated with the collagen molecule through hydrogen bonding. This significant feature of the prepared 3D collagen was revealed not only by the enhanced absorption of exudates but also by the support provided by the permeation of oxygen at the wound site [18,19].…”

Collagen/fibrin microbeads as a delivery system for Ag-doped bioactive glass and DPSCs for potential applications in dentistry

“…However, fibrin could not be used alone on fabricating robust cellular scaffolds due to low mechanical properties [26]. Additionally, collagen is of particular interest, as it is a naturally derived material, biodegradable and biocompatible [27,28], and has been investigated for numerous applications as well, such as in vascular [29], skin [30], ligament [31], and bone [32] tissue engineering. Applications of collagen in bone tissue regeneration showed that it acts as a bioactive component supporting cell attachment, proliferation and initiating osteogenic differentiation [14].…”