Fabrication of nanofibrous biomaterials based on natural materials (collagen, gelatin, etc.) through various techniques is an important research topic. Electrospinning, a well-established technique for nanofiber production has also been extended for producing nanofibrous structures of natural materials. Collagen nanofiber production utilizes hexafluoro isopropanol (HFIP) as a solvent for electrospinning. Research efforts are now focused on replacing HFIP with an environmentally benign solvent. In this study, electrospinning of Type I collagen of bovine skin with polycaprolactone (PCL) as a blend and an environmentally benign solvent, acetic acid, was carried out. The samples produced were subjected to contact angle measurements, porosity estimation, SEM, FTIR, TGA, and DSC. Nanofibers in the range of 100-200 nm were produced with an optimum porosity of 60%. The instrumental analyses confirm the physical interaction between collagen and PCL. Electrospinning of collagen in an environmentally benign solvent has been carried out and its usage in tissue engineering is being investigated by our research group.Correspondence to: A. Gnanamani (gnanamani3@gmail. com) and V. R. Giridev (Giridev vrgiridev@yahoo.com).

The present study summarizes the cross-linking property of succinic acid with chitosan /collagen. In detail, the chemistry behind the cross-linking and the improvement in mechanical and thermal properties of the cross-linked material were discussed with suitable instruments and bioinformatics tools. The concentration of succinic acid with reference to the chosen polymers was optimized. A 3D scaffold prepared using an optimized concentration of succinic acid (0.2% (w/v)) with chitosan (1.0% (w/v)) and similarly with collagen (0.5% (w/v)), was subjected to surface morphology, FT-IR analysis, tensile strength assessment, thermal stability and biocompatibility. Results revealed, cross-linking with succinic acid impart appreciable mechanical strength to the scaffold material. In silico analysis suggested the prevalence of non-covalent interactions, which played a crucial role in improving the mechanical and thermal properties of the cross-linked scaffold. The resultant 3D scaffold may find application as wound dressing material, as an implant in clinical applications and as a tissue engineering material.

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