Preparation and evaluation of novel stationary phases for improved chromatographic purification of pneumocandin B0

Welch, Christopher J.; DaSilva, Jimmy; Nti-Gyabaah, Joseph; Antia, Firoz D.; Göklen, Kent E.; Boyd, Russell

doi:10.1016/j.chroma.2005.10.009

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…It is possible that the interaction is influenced by the so‐called partitioning mechanism (20–26) . In a follow‐up study to explore the use of silica‐bonded phases (in which the bonded amine group was available for direct interactions) for the purification of pneumocandins, a significant increase in k ' for pneumocandin B 0 as well as for the two structural analogues was observed (29). It is possible that direct interactions of the pneumocandin hydroxyl with the bonded amine resulted in the retention increases.…”

Section: 0 Results and Discussionmentioning

confidence: 99%

Normal Phase High-Performance Liquid Chromatography of Pneumocandins: In Situ Modification of Silica with L-Proline To Separate Structural Analogues

Nti-Gyabaah¹,

Antia²,

Dahlgren³

et al. 2006

Biotechnol. Prog.

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Lipopeptides such as pneumocandin B(0) are often produced by fermentation processes. Many compounds with similar structures (structural analogues), and hence similar physiochemical properties, are coproduced in the fermentation. We employed high performance liquid chromatography using silica gel as the stationary phase and a ternary ethyl acetate/MeOH/water mobile phase to separate pneumocandin B(0) from these structural analogues. Despite extensive efforts to optimize this system, two key structural analogues, pneumocandin E(0) and pneumocandin B(5), continued to be poorly resolved from the main product peak (pneumocandin B(0)). As a result, feed load was restricted and productivity was limited. In situ modification of the silica gel stationary phase with l-proline or other amino acids significantly enhances the resolution of the two key structural analogues from the compound of interest, enabling a two-fold increase in productivity. Results of a systematic study showed that the amine group in l-proline and other amino acids plays a key role in the modification of the surface of the silica gel to mediate the selectivity enhancement.

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Section: 0 Results and Discussionmentioning

confidence: 99%

Normal Phase High-Performance Liquid Chromatography of Pneumocandins: In Situ Modification of Silica with L-Proline To Separate Structural Analogues

Nti-Gyabaah¹,

Antia²,

Dahlgren³

et al. 2006

Biotechnol. Prog.

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“…Achiral stationary phases evaluated in the study include Chromegabond Diol, Amine, Nitro, and Pyridyl Amide (ES Industries), Kromasil silica (Eka Chemicals), Princeton Benzamide, Pyridine, CPU, PPU, and PhPU (Princeton Chromatography) and an assortment of experimental stationary phases prepared in these laboratories. 10 …”

Section: Stationary Phasesmentioning

confidence: 97%

Solving multicomponent chiral separation challenges using a new SFC tandem column screening tool

et al. 2006

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A tool for improved tandem column chiral supercritical fluid chromatography (SFC) method development screening was prepared by modification of a commercial analytical SFC instrument with two different software-controllable, six position high-pressure column selection valves, each controlling a bank of five different columns and a pass through line. The resulting instrument, which has the ability to screen 10 different individual columns and 25 different tandem column arrangements, is a useful tool for facilitating the screening of tandem column SFC arrangements for separation of complex mixtures of stereoisomers or other multicomponent mixtures. Strategies for optimal use of the instrument are discussed, and several examples of the use of the instrument in developing tandem SFC methods for resolution of multicomponent mixtures are presented.

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Solubility and activity coefficient of pharmaceutical compounds in liquid organic solvents

Nti-Gyabaah¹

2009

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Preparation and evaluation of novel stationary phases for improved chromatographic purification of pneumocandin B0

Cited by 3 publications

References 13 publications

Normal Phase High-Performance Liquid Chromatography of Pneumocandins: In Situ Modification of Silica with L-Proline To Separate Structural Analogues

Normal Phase High-Performance Liquid Chromatography of Pneumocandins: In Situ Modification of Silica with L-Proline To Separate Structural Analogues

Solving multicomponent chiral separation challenges using a new SFC tandem column screening tool

Solubility and activity coefficient of pharmaceutical compounds in liquid organic solvents

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