Preparation and evaluation of multifunctional envelope-type nano device loading siRNA

Abstract

We prepared multifunctional envelope-type nano device (MEND) loading siRNA through lipid film hydration method; the qualities of MEND were characterized by the shape, particle size, and evaluated by the encapsulation efficiency (EE), plasma stability, and transfection efficiency. The formulated MEND was found to be relatively uniform in size with a positive zeta potential. The average siRNA entrapment efficiency was 86.4%. After 48 h exposure in 50% (v/v) serum at 37°C, the siRNA in MEND had no significant degradation. When the concentration of siRNA in 24-well plates was 500 nM, the MEND had high transfection efficiency, almost 100%.

Near-infrared light-activatable siRNA delivery by microcapsules for combined tumour therapy

“…We can easily see that the there is no visible band of lane of the siRNA treated with Rnase1, suggesting that the siRNA was damaged by the Rnase1. In contrast, the lane of MPA@siRNA@MC treated with Rnase1 without NIR laser irradiation still have bright band implying that the siRNA sequence attached to MPA@siRNA@MC was protected by the microcapsule so that it can be shielded from degradation [27]. Interestingly, the lane of MPA@siRNA@MC treated with Rnase1 with NIR laser irradiation have no visible band; therefore, we can get the conclusion that the NIR laser irradiation can damage the structure of microcapsule.…”

Measurement of serum prostate cancer markers using a nanopore thin film based optofluidic chip

“…Indeed, this study will have larger patient cohort and well-defined statistical objectives. Although these studies are preliminary, it has been demonstrated that this nanopore-based sensor, fabricated by a low-cost standard micro- and nano-fabrication process (Yin et al, 2014), can be a viable prototype for future prostate cancer diagnostics in the clinic. After full development, the nanopore-sensor enabled system can be essentially accessible to virtually any biomedical laboratory at a low cost.…”

“…This chip, consisting of arrayed nanopore-based sensors, has been fabricated using a newly developed process (Yin et al, 2014, Che et al, 2015). The fabrication process allows fabricating arrayed nanopore-based sensors on glass substrate in a very cost-efficient manner, rendering the disposable nanopore-based sensing chip possible.…”