Preparation and evaluation of collagen-silk fibroin/hydroxyapatite nanocomposites for bone tissue engineering

Chen, Li; Hu, Jingxiao; Ran, Jiabing; Shen, Xinyu; Tong, Hua

doi:10.1016/j.ijbiomac.2014.01.003

Cited by 80 publications

(44 citation statements)

References 47 publications

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“…However, the autograft is limited by the donor site morbidity, prolonged rehabilitation, increased risk of deep infection and restricted availability. Moreover, allografts might cause potential risks of transmitted diseases such as HIV or contamination [1,2]. Bone tissue engineering aims to develop bone graft replacements that can repair bone defects without a need for autografts [2].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to intra brillar mineral the surface of bril is coated by extra brillar mineral [7] Currently, there are some techniques concerning preparation of HA/Col composite materials, including coprecipitation, alternate soaking and mechanical mixing. Among these methods, there is a common shortcoming, that inorganic particles cannot be distributed homogeneously in the organic matrices at nanolevel, which leads to poor mechanical properties and limits their applications [1]. In this study, collagen molecule was prebrillated and then HA/Col nano-composites were synthesized by new biomimetic co-precipitation technique with the humidity conditions (pH value 7.48.0 and at 37…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Hydroxyapatite/Collagen (HA/COL) Composite Powder Using a Novel Precipitation Technique

Yoruç

Aydınoğlu

2015

Acta Phys. Pol. A

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A new biomimetic precipitation technique was developed to synthesize hydroxyapatite/collagen nanocomposites. The componential and morphological properties of nano-composites were investigated. It was revealed that the inorganic phase in the nano-composite was carbonate-substituted hydroxyapatite with low crystallinity. Indeed both characteristic peaks for HA and Col molecule and shifts of the characteristic peaks have indicated on HA-Col bonding. Morphology studies showed that hydroxyapatite particles with the average size of 50 nm were distributed homogeneously in the polymer matrix. According to the TEM micrographs, inorganic particles were needle-like and had regular crystallographic orientation. These results suggest that the hydroxyapatite/collagen nano-composites are promising biomaterials for bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis of Hydroxyapatite/Collagen (HA/COL) Composite Powder Using a Novel Precipitation Technique

Yoruç

Aydınoğlu

2015

Acta Phys. Pol. A

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“…The lower crystallinity of HAP in scaffolds suggests that the crystallographic structures were more similar to natural bone mineral (biological apatite). The lower crystallininty of HAP and reduced intensity of polysaccharides was due to the ionic interaction between amorphous polysaccharides and HAP as like collagen and HAP in natural bone mineral [20,42]. Morphology and porosity analysis: Porosity and pore size are important parameters for tissue engineering application to create a better cell proliferations, differentiations and adhesion.…”

Section: Resultsmentioning

confidence: 99%

Development of Porous Polysaccharides-Hydroxyapatite Composite Scaffolds via Freeze Drying Method and in vitro Osteoconductivity on MG 63 Cells

Rajesh¹,

Kumar²,

Kenie³

et al. 2018

Asian J. Chem.

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“…We left the final mixture standing for 24 h to complete the crosslinking and to stabilize the polymer before using it [22][23][24][25].…”

Section: Preparation Of Injectable Bone Substitutes Sf/ Nhamentioning

confidence: 99%

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Buitrago-Vásquez

Orozco

2018

Rev. Fac. Ing.

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Injectable bone substitutes are generally composite materials capable of being extruded through a device; they consist of a solid phase inside a matrix that allows the easy movement of particles. Injectable bone substitutes have the advantage of allowing its application in situ without the need of invasive surgical techniques, guaranteeing a good recovery; for this reason, they are a promising alternative to replace conventional techniques to repair bone defects. Conventional techniques include the use of allografts and autografts, which often cause adverse reactions, and are disadvantageous for both the patient and the doctor. Furthermore, there are no reports regarding bone substitute development in Colombia, creating the necessity to research composite materials that could become injectable bone substitutes. In this study, we manufactured injectable bone substitutes with hydroxyapatite and calcium phosphate, which is most similar to bone tissue, and synthesized them in nanorods with shape and size similar to the natural hydroxyapatite found inside the body. Additionally, we used extracted silk fibroin from silkworm cocoons of Bombyx mori, a natural polymer of protein nature with high mechanical properties and excellent biocompatibility. For the materials manufactured, we evaluated degradation, in a simulated body fluid (SBF) at normal body temperature, water uptake, injectability and mechanical strength. The manufactured bone substitutes showed good degradation and water uptake properties, an approximate 97% injectability, and low mechanical resistance, indicating promising properties to be used as an injectable bone substitute.

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Preparation and evaluation of collagen-silk fibroin/hydroxyapatite nanocomposites for bone tissue engineering

Cited by 80 publications

References 47 publications

Synthesis of Hydroxyapatite/Collagen (HA/COL) Composite Powder Using a Novel Precipitation Technique

Synthesis of Hydroxyapatite/Collagen (HA/COL) Composite Powder Using a Novel Precipitation Technique

Development of Porous Polysaccharides-Hydroxyapatite Composite Scaffolds via Freeze Drying Method and in vitro Osteoconductivity on MG 63 Cells

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

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