Preparation and evaluation of chitosan/alginate porous microspheres/Bletilla striata polysaccharide composite hemostatic sponges

Wang, Chao; Luo, Wenfeng; Li, Puwang; Li, Sidong; Yang, Ziming; Zhang, Hu; Liu, Yangyang; Ao, Ningjian

doi:10.1016/j.carbpol.2017.06.112

Cited by 145 publications

(63 citation statements)

References 54 publications

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“…Accumulating evidence has revealed that BSP can induce cell proliferation in different types of cells such as HUVEC, HCEC, L929 and 3T3 [29] and even HTs (in this research). However, the underlaying molecular mechanisms remain to be fully elucidated.…”

Section: Discussionsupporting

confidence: 53%

Polysaccharide Extracted from Bletilla striata Promotes Proliferation and Migration of Human Tenocytes

Chen

et al. 2020

Polymers

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Tendon healing after injury is relatively slow, mainly because of the weak activity and metabolic properties of tendon cells (tenocytes). Bletilla striata polysaccharide (BSP) has been reported to enhance cell proliferation. Here, we aimed to increase tendon cell proliferation by BSP treatment. We isolated tenocytes from the flexor tendon of human origin. Moreover, we improved the process of extracting BSP. When human tenocytes (HTs) were treated with 100 μg/mL BSP, the MEK/ERK1/2 and PI3K/Akt signaling pathways were activated, thereby enhancing the proliferation ability of tenocytes. BSP treatment also increased the migration of HTs and their ability to secrete the extracellular matrix (Col-I and Col-III). In conclusion, BSP was successfully extracted from a natural Chinese herbal extract and was shown to enhance tenocytes proliferation, migration and collagen release ability. This study is the first to demonstrate improved healing of tendons using BSP.

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Section: Discussionsupporting

confidence: 53%

Polysaccharide Extracted from Bletilla striata Promotes Proliferation and Migration of Human Tenocytes

Chen

et al. 2020

Polymers

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“…The fluid absorption would be cooperative in controlling asepsis at the post-extractive sockets [ 58 ]. Moreover, porosity causes an improvement in cell gas exchange, epithelium regeneration, exudates removal, and hemostasis [ 59 , 60 ]. By investigation, the porosity of Sponge-C and Sponge-C-MTR resulted in 93.15 ± 0.14% and 92.86 ± 0.18%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Development of a Multifunctional Bioerodible Nanocomposite Containing Metronidazole and Curcumin to Apply on L-PRF Clot to Promote Tissue Regeneration in Dentistry

et al. 2020

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Teeth extractions are often followed by alveolar bone reabsorption, although an adequate level of bone is required for reliable rehabilitations by dental implants. Leukocyte and platelet-rich fibrin (L-PRF) has been widely applied in regenerative procedures and with antibiotic and antioxidant agents could play an essential role in hard and soft tissue healing. In this work, a nanocomposite (Sponge-C-MTR) consisting of a hyaluronate-based sponge loaded with metronidazole (MTR) and nanostructured lipid carriers containing curcumin (CUR-NLC) was designed to be wrapped in the L-PRF™ membrane in the post-extraction sockets and characterized. CUR-NLCs, obtained by homogenization followed by high-frequency sonication of the lipid mixture, showed loading capacity (5% w/w), drug recovery (95% w/w), spherical shape with an average particle size of 112.0 nm, and Zeta potential of −24 mV. Sponge-C-MTR was obtained by entrapping CUR-NLC in a hydrophilic matrix by a freeze-drying process, and physico-chemical and cytocompatibility properties were evaluated. Moreover, the aptitude of CUR and MTR to the penetrate and/or permeate both L-PRF™ and porcine buccal tissue was assessed, highlighting MTR penetration and CUR accumulation promoted by the system. The results positively support the action of nanocomposite in dental tissues regeneration when applied together with the L-PRF™.

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“…Hemostasis was an extremely significant process that prevents abundant blood loss after vascular injury . Blood coagulation was an extremely complicated and dynamic physiological process .…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, many materials with excellent biocompatibility, such as calcium chloride, genipin, and sodium alginate, had been explored as cross‐linkers to stabilize the structure of microparticles . Recently, tannic acid (TA), a plant polyphenol of natural origin, had emerged as a pyrogallol containing cross‐linker.…”

Section: Introductionmentioning

confidence: 99%

Tannic Acid Cross‐linked Polysaccharide‐Based Multifunctional Hemostatic Microparticles for the Regulation of Rapid Wound Healing

Xiao

et al. 2018

Macromolecular Bioscience

100

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Hemostatic microparticles (HMs) have been widely used in surgery. To improve the comprehensive performance of HMs, multifunctional HMs named HM15 and HM15′ are prepared from starch, carboxymethyl chitosan, hyaluronic acid, and tannic acid. Herein, tannic acid is used as an effective cross‐linker. A 3D network structure for cell growth and wound repair can be formed by secondary cross‐linking. Through synergistic effect of these natural materials, the process of wound healing can be regulated controllably. HM15 and HM15′ have the ability of rapid hemostasis. Moreover, HM15′ shows excellent properties in antibacteria and wound healing acceleration. Blood clotting time treated with different HMs is shortened obviously from 436.8 s to 126 s. Compared with Celox, HM15 and HM15′ exhibited better broad spectrum antibacterial activity against both Escherichia coli and Staphylococcus aureus. Notably, the wound can be repaired rapidly by HM15′ in 14 days. These multifunctional HMs might have an important prospect in clinical application.

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Preparation and evaluation of chitosan/alginate porous microspheres/Bletilla striata polysaccharide composite hemostatic sponges

Cited by 145 publications

References 54 publications

Polysaccharide Extracted from Bletilla striata Promotes Proliferation and Migration of Human Tenocytes

Polysaccharide Extracted from Bletilla striata Promotes Proliferation and Migration of Human Tenocytes

Development of a Multifunctional Bioerodible Nanocomposite Containing Metronidazole and Curcumin to Apply on L-PRF Clot to Promote Tissue Regeneration in Dentistry

Tannic Acid Cross‐linked Polysaccharide‐Based Multifunctional Hemostatic Microparticles for the Regulation of Rapid Wound Healing

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